Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF750 and GFF751 are separated by 6 nucleotides GFF751 and GFF752 are separated by 36 nucleotides GFF752 and GFF753 are separated by 130 nucleotides GFF753 and GFF754 are separated by 156 nucleotides
GFF750 - Transcriptional regulator LigR, LysR family, at 13,647 to 14,924
GFF750
GFF751 - probable acetyltransferase protein, at 14,931 to 15,380
GFF751
GFF752 - hypothetical protein, at 15,417 to 15,758
GFF752
GFF753 - Methyl-accepting chemotaxis sensor/transducer protein, at 15,889 to 17,532
GFF753
GFF754 - FIG00975093: hypothetical protein, at 17,689 to 18,522
GFF754
Position (kb) 15
16
17
18 Strain fitness (log2 ratio) -2
-1
0
1
2 at 14.996 kb on - strand, within GFF751 at 14.996 kb on - strand, within GFF751 at 14.996 kb on - strand, within GFF751 at 15.046 kb on + strand, within GFF751 at 15.046 kb on + strand, within GFF751 at 15.046 kb on + strand, within GFF751 at 15.047 kb on - strand, within GFF751 at 15.152 kb on - strand, within GFF751 at 15.236 kb on - strand, within GFF751 at 15.248 kb on + strand, within GFF751 at 15.248 kb on + strand, within GFF751 at 15.249 kb on - strand, within GFF751 at 15.328 kb on + strand, within GFF751 at 15.329 kb on - strand, within GFF751 at 15.329 kb on - strand, within GFF751 at 15.372 kb on + strand at 15.372 kb on + strand at 15.372 kb on + strand at 15.372 kb on + strand at 15.373 kb on - strand at 15.536 kb on + strand, within GFF752 at 15.536 kb on + strand, within GFF752 at 15.536 kb on + strand, within GFF752 at 15.536 kb on + strand, within GFF752 at 15.536 kb on + strand, within GFF752 at 15.537 kb on - strand, within GFF752 at 15.537 kb on - strand, within GFF752 at 15.537 kb on - strand, within GFF752 at 15.537 kb on - strand, within GFF752 at 15.537 kb on - strand, within GFF752 at 15.623 kb on - strand, within GFF752 at 15.677 kb on - strand, within GFF752 at 16.812 kb on - strand, within GFF753 at 16.812 kb on - strand, within GFF753 at 17.057 kb on + strand, within GFF753 at 17.057 kb on + strand, within GFF753 at 17.057 kb on + strand, within GFF753 at 17.057 kb on + strand, within GFF753 at 17.057 kb on + strand, within GFF753 at 17.057 kb on + strand, within GFF753 at 17.058 kb on - strand, within GFF753 at 17.058 kb on - strand, within GFF753 at 17.058 kb on - strand, within GFF753 at 17.058 kb on - strand, within GFF753 at 17.160 kb on - strand, within GFF753 at 17.160 kb on - strand, within GFF753 at 17.285 kb on + strand, within GFF753 at 17.349 kb on + strand, within GFF753 at 17.350 kb on - strand, within GFF753 at 17.440 kb on + strand at 17.467 kb on + strand at 17.467 kb on + strand at 17.468 kb on - strand at 17.546 kb on + strand at 17.546 kb on + strand at 17.637 kb on - strand at 17.660 kb on + strand at 17.664 kb on + strand at 17.881 kb on + strand, within GFF754 at 17.881 kb on + strand, within GFF754 at 17.882 kb on - strand, within GFF754 at 18.000 kb on - strand, within GFF754 at 18.194 kb on + strand, within GFF754 at 18.194 kb on + strand, within GFF754 at 18.195 kb on - strand, within GFF754 at 18.195 kb on - strand, within GFF754 at 18.281 kb on + strand, within GFF754 at 18.281 kb on + strand, within GFF754 at 18.281 kb on + strand, within GFF754 at 18.323 kb on + strand, within GFF754 at 18.324 kb on - strand, within GFF754 at 18.330 kb on - strand, within GFF754 at 18.365 kb on + strand, within GFF754 at 18.365 kb on + strand, within GFF754 at 18.365 kb on + strand, within GFF754 at 18.365 kb on + strand, within GFF754 at 18.366 kb on - strand, within GFF754 at 18.366 kb on - strand, within GFF754 at 18.366 kb on - strand, within GFF754 at 18.366 kb on - strand, within GFF754 at 18.366 kb on - strand, within GFF754 at 18.366 kb on - strand, within GFF754
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 14,996 - GFF751 0.14 -0.2 14,996 - GFF751 0.14 -0.5 14,996 - GFF751 0.14 +0.5 15,046 + GFF751 0.26 -0.1 15,046 + GFF751 0.26 +0.9 15,046 + GFF751 0.26 +0.5 15,047 - GFF751 0.26 -0.1 15,152 - GFF751 0.49 -0.5 15,236 - GFF751 0.68 -0.3 15,248 + GFF751 0.70 -2.0 15,248 + GFF751 0.70 -0.2 15,249 - GFF751 0.71 -1.2 15,328 + GFF751 0.88 -0.8 15,329 - GFF751 0.88 -0.1 15,329 - GFF751 0.88 +1.3 15,372 + +1.5 15,372 + +1.7 15,372 + -0.5 15,372 + -1.4 15,373 - -1.5 15,536 + GFF752 0.35 -0.4 15,536 + GFF752 0.35 +0.7 15,536 + GFF752 0.35 -1.0 15,536 + GFF752 0.35 +0.3 15,536 + GFF752 0.35 -0.6 15,537 - GFF752 0.35 -1.1 15,537 - GFF752 0.35 -0.3 15,537 - GFF752 0.35 -0.7 15,537 - GFF752 0.35 -0.2 15,537 - GFF752 0.35 -0.8 15,623 - GFF752 0.60 +1.0 15,677 - GFF752 0.76 -0.2 16,812 - GFF753 0.56 -0.2 16,812 - GFF753 0.56 -0.4 17,057 + GFF753 0.71 -1.2 17,057 + GFF753 0.71 -1.5 17,057 + GFF753 0.71 +0.5 17,057 + GFF753 0.71 -0.3 17,057 + GFF753 0.71 +0.0 17,057 + GFF753 0.71 -0.6 17,058 - GFF753 0.71 -0.6 17,058 - GFF753 0.71 -0.3 17,058 - GFF753 0.71 +0.2 17,058 - GFF753 0.71 -1.6 17,160 - GFF753 0.77 +2.5 17,160 - GFF753 0.77 +0.0 17,285 + GFF753 0.85 -0.5 17,349 + GFF753 0.89 -1.6 17,350 - GFF753 0.89 -1.0 17,440 + +0.3 17,467 + -0.4 17,467 + -0.5 17,468 - -0.8 17,546 + +0.3 17,546 + +0.5 17,637 - -0.2 17,660 + +0.4 17,664 + -0.6 17,881 + GFF754 0.23 -0.6 17,881 + GFF754 0.23 +1.2 17,882 - GFF754 0.23 +0.7 18,000 - GFF754 0.37 -0.6 18,194 + GFF754 0.61 -0.0 18,194 + GFF754 0.61 +0.4 18,195 - GFF754 0.61 +0.7 18,195 - GFF754 0.61 -0.6 18,281 + GFF754 0.71 -1.5 18,281 + GFF754 0.71 -0.6 18,281 + GFF754 0.71 -0.5 18,323 + GFF754 0.76 -0.1 18,324 - GFF754 0.76 -0.2 18,330 - GFF754 0.77 -0.5 18,365 + GFF754 0.81 -0.5 18,365 + GFF754 0.81 -0.3 18,365 + GFF754 0.81 -0.5 18,365 + GFF754 0.81 +0.3 18,366 - GFF754 0.81 -1.4 18,366 - GFF754 0.81 +0.9 18,366 - GFF754 0.81 -1.3 18,366 - GFF754 0.81 +0.1 18,366 - GFF754 0.81 +0.3 18,366 - GFF754 0.81 -0.5
Or see this region's nucleotide sequence