Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6803 and GFF6804 are separated by 30 nucleotides GFF6804 and GFF6805 overlap by 4 nucleotides GFF6805 and GFF6806 are separated by 142 nucleotides GFF6806 and GFF6807 are separated by 16 nucleotides
GFF6803 - N-acyl homoserine lactone hydrolase, at 50,144 to 51,217
GFF6803
GFF6804 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 51,248 to 52,021
GFF6804
GFF6805 - FIG00821908: hypothetical protein, at 52,018 to 52,524
GFF6805
GFF6806 - transcriptional regulator, Crp/Fnr family, at 52,667 to 53,410
GFF6806
GFF6807 - BUG/TctC family periplasmic protein, at 53,427 to 54,392
GFF6807
Position (kb) 52
53 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 51.252 kb on + strand at 51.543 kb on + strand, within GFF6804 at 51.700 kb on + strand, within GFF6804 at 51.700 kb on + strand, within GFF6804 at 51.700 kb on + strand, within GFF6804 at 51.701 kb on - strand, within GFF6804 at 51.701 kb on - strand, within GFF6804 at 52.145 kb on + strand, within GFF6805 at 52.352 kb on + strand, within GFF6805 at 52.352 kb on + strand, within GFF6805 at 52.353 kb on - strand, within GFF6805 at 52.353 kb on - strand, within GFF6805 at 52.353 kb on - strand, within GFF6805 at 52.355 kb on + strand, within GFF6805 at 52.409 kb on + strand, within GFF6805 at 52.410 kb on - strand, within GFF6805 at 52.600 kb on + strand at 52.600 kb on + strand at 52.601 kb on - strand at 53.518 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 51,252 + +0.4 51,543 + GFF6804 0.38 -0.4 51,700 + GFF6804 0.58 -1.1 51,700 + GFF6804 0.58 -0.0 51,700 + GFF6804 0.58 -0.1 51,701 - GFF6804 0.59 +0.3 51,701 - GFF6804 0.59 +2.3 52,145 + GFF6805 0.25 +1.2 52,352 + GFF6805 0.66 -0.5 52,352 + GFF6805 0.66 +4.5 52,353 - GFF6805 0.66 +0.4 52,353 - GFF6805 0.66 -1.1 52,353 - GFF6805 0.66 -0.7 52,355 + GFF6805 0.66 -0.1 52,409 + GFF6805 0.77 +1.2 52,410 - GFF6805 0.77 +0.9 52,600 + -1.1 52,600 + +0.0 52,601 - -1.7 53,518 + -0.5
Or see this region's nucleotide sequence