Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6548 and GFF6549 are separated by 22 nucleotides GFF6549 and GFF6550 are separated by 163 nucleotides GFF6550 and GFF6551 are separated by 30 nucleotides GFF6551 and GFF6552 are separated by 16 nucleotides
GFF6548 - hypothetical protein, at 45,940 to 46,593
GFF6548
GFF6549 - no description, at 46,616 to 47,392
GFF6549
GFF6550 - 4'-phosphopantetheinyl transferase (EC 2.7.8.-), at 47,556 to 48,371
GFF6550
GFF6551 - Plasmid replication/partition related protein, at 48,402 to 49,244
GFF6551
GFF6552 - Uncharacterized lipoprotein Bmul_4930, at 49,261 to 49,845
GFF6552
Position (kb) 47
48
49 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 46.647 kb on + strand at 46.648 kb on - strand at 46.648 kb on - strand at 46.740 kb on + strand, within GFF6549 at 46.740 kb on + strand, within GFF6549 at 46.740 kb on + strand, within GFF6549 at 46.740 kb on + strand, within GFF6549 at 46.741 kb on - strand, within GFF6549 at 46.741 kb on - strand, within GFF6549 at 46.741 kb on - strand, within GFF6549 at 46.741 kb on - strand, within GFF6549 at 47.139 kb on + strand, within GFF6549 at 47.139 kb on + strand, within GFF6549 at 47.139 kb on + strand, within GFF6549 at 47.139 kb on + strand, within GFF6549 at 47.139 kb on + strand, within GFF6549 at 47.139 kb on + strand, within GFF6549 at 47.140 kb on - strand, within GFF6549 at 47.140 kb on - strand, within GFF6549 at 47.432 kb on + strand at 47.441 kb on + strand at 47.441 kb on + strand at 47.599 kb on + strand at 47.749 kb on + strand, within GFF6550 at 47.749 kb on + strand, within GFF6550 at 47.750 kb on - strand, within GFF6550 at 47.750 kb on - strand, within GFF6550 at 47.769 kb on + strand, within GFF6550 at 47.770 kb on - strand, within GFF6550 at 47.870 kb on - strand, within GFF6550 at 48.075 kb on + strand, within GFF6550 at 48.075 kb on + strand, within GFF6550 at 48.075 kb on + strand, within GFF6550 at 48.075 kb on + strand, within GFF6550 at 48.075 kb on + strand, within GFF6550 at 48.075 kb on + strand, within GFF6550 at 48.075 kb on + strand, within GFF6550 at 48.075 kb on + strand, within GFF6550 at 48.075 kb on + strand, within GFF6550 at 48.075 kb on + strand, within GFF6550 at 48.076 kb on - strand, within GFF6550 at 48.076 kb on - strand, within GFF6550 at 48.076 kb on - strand, within GFF6550 at 48.076 kb on - strand, within GFF6550 at 48.076 kb on - strand, within GFF6550 at 48.076 kb on - strand, within GFF6550 at 48.076 kb on - strand, within GFF6550 at 48.370 kb on - strand at 48.370 kb on - strand at 48.435 kb on + strand at 48.435 kb on + strand at 48.435 kb on + strand at 48.435 kb on + strand at 48.435 kb on + strand at 48.435 kb on + strand at 48.435 kb on + strand at 48.435 kb on + strand at 48.436 kb on - strand at 48.436 kb on - strand at 48.436 kb on - strand at 48.436 kb on - strand at 48.436 kb on - strand at 48.438 kb on - strand at 48.462 kb on + strand at 48.462 kb on + strand at 48.463 kb on - strand at 48.463 kb on - strand at 48.463 kb on - strand at 49.262 kb on + strand at 49.262 kb on + strand at 49.262 kb on + strand at 49.263 kb on - strand at 49.263 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 46,647 + +0.5 46,648 - +0.3 46,648 - +0.7 46,740 + GFF6549 0.16 -0.2 46,740 + GFF6549 0.16 -0.6 46,740 + GFF6549 0.16 -3.6 46,740 + GFF6549 0.16 -0.4 46,741 - GFF6549 0.16 -0.5 46,741 - GFF6549 0.16 +1.1 46,741 - GFF6549 0.16 -0.1 46,741 - GFF6549 0.16 -0.1 47,139 + GFF6549 0.67 -0.1 47,139 + GFF6549 0.67 -0.5 47,139 + GFF6549 0.67 -0.8 47,139 + GFF6549 0.67 -1.2 47,139 + GFF6549 0.67 -0.1 47,139 + GFF6549 0.67 +0.4 47,140 - GFF6549 0.67 +0.1 47,140 - GFF6549 0.67 -0.1 47,432 + -0.7 47,441 + -0.1 47,441 + -1.1 47,599 + -0.1 47,749 + GFF6550 0.24 -1.0 47,749 + GFF6550 0.24 -0.5 47,750 - GFF6550 0.24 +0.9 47,750 - GFF6550 0.24 +0.0 47,769 + GFF6550 0.26 +0.1 47,770 - GFF6550 0.26 -0.8 47,870 - GFF6550 0.38 +0.1 48,075 + GFF6550 0.64 +1.2 48,075 + GFF6550 0.64 -0.1 48,075 + GFF6550 0.64 -1.7 48,075 + GFF6550 0.64 +0.3 48,075 + GFF6550 0.64 +0.3 48,075 + GFF6550 0.64 -0.5 48,075 + GFF6550 0.64 -0.4 48,075 + GFF6550 0.64 -1.1 48,075 + GFF6550 0.64 -0.0 48,075 + GFF6550 0.64 -1.3 48,076 - GFF6550 0.64 +0.0 48,076 - GFF6550 0.64 -0.3 48,076 - GFF6550 0.64 -0.8 48,076 - GFF6550 0.64 -0.7 48,076 - GFF6550 0.64 -2.8 48,076 - GFF6550 0.64 -0.5 48,076 - GFF6550 0.64 -0.7 48,370 - +1.0 48,370 - -1.2 48,435 + -0.6 48,435 + -0.7 48,435 + +0.2 48,435 + -0.1 48,435 + +1.2 48,435 + -0.6 48,435 + -0.3 48,435 + -0.2 48,436 - -0.5 48,436 - +1.6 48,436 - -1.7 48,436 - -1.2 48,436 - -0.3 48,438 - -0.6 48,462 + +0.1 48,462 + -0.4 48,463 - +0.1 48,463 - -1.8 48,463 - -0.0 49,262 + +0.6 49,262 + +1.7 49,262 + -1.4 49,263 - -0.3 49,263 - -0.5
Or see this region's nucleotide sequence