Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6361 and GFF6362 overlap by 4 nucleotides GFF6362 and GFF6363 overlap by 4 nucleotides GFF6363 and GFF6364 are separated by 7 nucleotides
GFF6361 - Pentapeptide repeat family protein, at 25,920 to 26,990
GFF6361
GFF6362 - Pentapeptide repeat family protein, at 26,987 to 29,590
GFF6362
GFF6363 - hypothetical protein, at 29,587 to 29,982
GFF6363
GFF6364 - VgrG protein, at 29,990 to 32,356
GFF6364
Position (kb) 26
27
28
29
30 Strain fitness (log2 ratio) -2
-1
0
1 at 27.043 kb on - strand at 27.076 kb on - strand at 27.237 kb on - strand at 27.578 kb on + strand, within GFF6362 at 27.826 kb on + strand, within GFF6362 at 28.047 kb on + strand, within GFF6362 at 28.048 kb on - strand, within GFF6362 at 28.938 kb on + strand, within GFF6362 at 28.998 kb on + strand, within GFF6362 at 28.998 kb on + strand, within GFF6362 at 28.998 kb on + strand, within GFF6362 at 29.269 kb on - strand, within GFF6362 at 29.358 kb on + strand at 29.359 kb on - strand at 29.359 kb on - strand at 29.359 kb on - strand at 29.765 kb on + strand, within GFF6363 at 29.765 kb on + strand, within GFF6363 at 29.765 kb on + strand, within GFF6363 at 29.765 kb on + strand, within GFF6363 at 29.765 kb on + strand, within GFF6363 at 29.765 kb on + strand, within GFF6363 at 29.766 kb on - strand, within GFF6363 at 29.766 kb on - strand, within GFF6363 at 29.806 kb on + strand, within GFF6363 at 29.806 kb on + strand, within GFF6363 at 29.807 kb on - strand, within GFF6363 at 29.960 kb on + strand at 29.967 kb on - strand at 30.019 kb on - strand at 30.048 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 27,043 - -0.3 27,076 - -0.4 27,237 - +0.4 27,578 + GFF6362 0.23 +0.3 27,826 + GFF6362 0.32 -0.9 28,047 + GFF6362 0.41 -0.5 28,048 - GFF6362 0.41 -0.6 28,938 + GFF6362 0.75 -0.2 28,998 + GFF6362 0.77 -0.2 28,998 + GFF6362 0.77 +0.2 28,998 + GFF6362 0.77 -1.4 29,269 - GFF6362 0.88 -0.7 29,358 + -1.0 29,359 - -0.5 29,359 - +0.2 29,359 - -0.1 29,765 + GFF6363 0.45 -0.1 29,765 + GFF6363 0.45 +1.3 29,765 + GFF6363 0.45 +0.2 29,765 + GFF6363 0.45 +0.5 29,765 + GFF6363 0.45 -1.3 29,765 + GFF6363 0.45 +0.3 29,766 - GFF6363 0.45 +0.5 29,766 - GFF6363 0.45 -0.5 29,806 + GFF6363 0.55 +0.1 29,806 + GFF6363 0.55 +0.1 29,807 - GFF6363 0.56 -0.8 29,960 + -0.7 29,967 - +0.8 30,019 - -1.9 30,048 + -0.4
Or see this region's nucleotide sequence