Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6257 and GFF6258 overlap by 4 nucleotides GFF6258 and GFF6259 overlap by 4 nucleotides GFF6259 and GFF6260 are separated by 1 nucleotides GFF6260 and GFF6261 overlap by 4 nucleotides
GFF6257 - Phosphonates utilization ATP-binding protein PhnK, at 50,695 to 51,483
GFF6257
GFF6258 - Alpha-D-ribose 1-methylphosphonate 5-phosphate C-P lyase (EC 4.7.1.1), at 51,480 to 52,352
GFF6258
GFF6259 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnI (EC 2.7.8.37), at 52,349 to 53,446
GFF6259
GFF6260 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnH (EC 2.7.8.37), at 53,448 to 54,077
GFF6260
GFF6261 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnG (EC 2.7.8.37), at 54,074 to 54,577
GFF6261
Position (kb) 52
53
54 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 51.426 kb on - strand at 51.697 kb on + strand, within GFF6258 at 51.698 kb on - strand, within GFF6258 at 51.713 kb on - strand, within GFF6258 at 51.713 kb on - strand, within GFF6258 at 51.713 kb on - strand, within GFF6258 at 51.713 kb on - strand, within GFF6258 at 51.841 kb on + strand, within GFF6258 at 51.841 kb on + strand, within GFF6258 at 51.841 kb on + strand, within GFF6258 at 51.841 kb on + strand, within GFF6258 at 51.841 kb on + strand, within GFF6258 at 51.841 kb on + strand, within GFF6258 at 51.842 kb on - strand, within GFF6258 at 51.842 kb on - strand, within GFF6258 at 51.880 kb on + strand, within GFF6258 at 51.986 kb on - strand, within GFF6258 at 52.322 kb on - strand at 52.322 kb on - strand at 52.333 kb on + strand at 52.333 kb on + strand at 52.333 kb on + strand at 52.334 kb on - strand at 52.419 kb on + strand at 52.419 kb on + strand at 52.419 kb on + strand at 52.419 kb on + strand at 52.419 kb on + strand at 52.419 kb on + strand at 52.419 kb on + strand at 52.419 kb on + strand at 52.603 kb on - strand, within GFF6259 at 52.794 kb on + strand, within GFF6259 at 52.795 kb on - strand, within GFF6259 at 52.932 kb on - strand, within GFF6259 at 52.932 kb on - strand, within GFF6259 at 53.076 kb on + strand, within GFF6259 at 53.077 kb on - strand, within GFF6259 at 53.077 kb on - strand, within GFF6259 at 53.082 kb on + strand, within GFF6259 at 53.082 kb on + strand, within GFF6259 at 53.082 kb on + strand, within GFF6259 at 53.083 kb on - strand, within GFF6259 at 53.083 kb on - strand, within GFF6259 at 53.206 kb on - strand, within GFF6259 at 53.283 kb on + strand, within GFF6259 at 53.283 kb on + strand, within GFF6259 at 53.283 kb on + strand, within GFF6259 at 53.283 kb on + strand, within GFF6259 at 53.284 kb on - strand, within GFF6259 at 53.401 kb on - strand at 53.401 kb on - strand at 53.401 kb on - strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 54.303 kb on + strand, within GFF6261 at 54.303 kb on + strand, within GFF6261 at 54.304 kb on - strand, within GFF6261 at 54.304 kb on - strand, within GFF6261
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 51,426 - -0.5 51,697 + GFF6258 0.25 +0.1 51,698 - GFF6258 0.25 -1.2 51,713 - GFF6258 0.27 +1.2 51,713 - GFF6258 0.27 -0.1 51,713 - GFF6258 0.27 +0.3 51,713 - GFF6258 0.27 -0.4 51,841 + GFF6258 0.41 +0.1 51,841 + GFF6258 0.41 -1.1 51,841 + GFF6258 0.41 -1.5 51,841 + GFF6258 0.41 -0.3 51,841 + GFF6258 0.41 -0.5 51,841 + GFF6258 0.41 -0.1 51,842 - GFF6258 0.41 -0.6 51,842 - GFF6258 0.41 -2.5 51,880 + GFF6258 0.46 -0.6 51,986 - GFF6258 0.58 +3.1 52,322 - -0.1 52,322 - +1.8 52,333 + -0.4 52,333 + -0.4 52,333 + +0.9 52,334 - -0.1 52,419 + -0.8 52,419 + -0.4 52,419 + +0.6 52,419 + -0.2 52,419 + +1.0 52,419 + -0.1 52,419 + -0.0 52,419 + +1.0 52,603 - GFF6259 0.23 -0.6 52,794 + GFF6259 0.41 +0.9 52,795 - GFF6259 0.41 -0.4 52,932 - GFF6259 0.53 -0.5 52,932 - GFF6259 0.53 -0.0 53,076 + GFF6259 0.66 -0.8 53,077 - GFF6259 0.66 -0.5 53,077 - GFF6259 0.66 -1.0 53,082 + GFF6259 0.67 -0.5 53,082 + GFF6259 0.67 -1.2 53,082 + GFF6259 0.67 -0.2 53,083 - GFF6259 0.67 -0.0 53,083 - GFF6259 0.67 -1.5 53,206 - GFF6259 0.78 -1.5 53,283 + GFF6259 0.85 -1.9 53,283 + GFF6259 0.85 -0.2 53,283 + GFF6259 0.85 -0.6 53,283 + GFF6259 0.85 -0.3 53,284 - GFF6259 0.85 +1.0 53,401 - +0.5 53,401 - +1.2 53,401 - -0.7 53,442 + +0.1 53,442 + -0.5 53,442 + -0.2 53,442 + +0.2 53,442 + -1.0 53,442 + +1.2 53,442 + -2.0 53,442 + -0.7 53,442 + -0.5 53,442 + -0.1 53,442 + -0.1 53,442 + +0.5 53,442 + -0.5 53,442 + +0.3 53,442 + +0.8 53,442 + -0.1 53,443 - -1.1 53,443 - -0.2 53,443 - +0.4 53,443 - -1.1 53,443 - -0.7 53,443 - -0.5 53,443 - -0.5 53,443 - -1.8 53,443 - -1.7 53,443 - -0.6 53,443 - +0.8 53,443 - -0.2 53,443 - -0.8 53,443 - +1.4 53,443 - +0.5 53,443 - -0.5 53,443 - -1.9 54,303 + GFF6261 0.45 +0.0 54,303 + GFF6261 0.45 -0.9 54,304 - GFF6261 0.46 -0.1 54,304 - GFF6261 0.46 +0.5
Or see this region's nucleotide sequence