Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5907 and GFF5908 are separated by 2 nucleotides GFF5908 and GFF5909 overlap by 4 nucleotides GFF5909 and GFF5910 overlap by 10 nucleotides GFF5910 and GFF5911 are separated by 37 nucleotides
GFF5907 - Biotin synthase-related enzyme, at 123,055 to 124,137
GFF5907
GFF5908 - Histone acetyltransferase HPA2 and related acetyltransferases, at 124,140 to 124,700
GFF5908
GFF5909 - Selenophosphate synthetase-related protein MJ0640, at 124,697 to 125,704
GFF5909
GFF5910 - NAD/NADP transhydrogenase alpha subunit, at 125,695 to 125,979
GFF5910
GFF5911 - Probable L-amino-acid oxidase (EC 1.4.3.2), at 126,017 to 127,324
GFF5911
Position (kb) 124
125
126 Strain fitness (log2 ratio) -2
-1
0
1
2 at 123.853 kb on + strand, within GFF5907 at 123.853 kb on + strand, within GFF5907 at 124.112 kb on + strand at 124.114 kb on + strand at 124.114 kb on + strand at 124.115 kb on - strand at 124.180 kb on - strand at 124.195 kb on - strand at 124.877 kb on + strand, within GFF5909 at 124.944 kb on - strand, within GFF5909 at 124.976 kb on + strand, within GFF5909 at 124.976 kb on + strand, within GFF5909 at 124.976 kb on + strand, within GFF5909 at 124.977 kb on - strand, within GFF5909 at 124.977 kb on - strand, within GFF5909 at 124.977 kb on - strand, within GFF5909 at 125.235 kb on - strand, within GFF5909 at 125.798 kb on - strand, within GFF5910 at 126.048 kb on - strand at 126.407 kb on + strand, within GFF5911 at 126.516 kb on - strand, within GFF5911 at 126.516 kb on - strand, within GFF5911 at 126.659 kb on + strand, within GFF5911 at 126.660 kb on - strand, within GFF5911 at 126.701 kb on + strand, within GFF5911 at 126.702 kb on - strand, within GFF5911 at 126.702 kb on - strand, within GFF5911 at 126.702 kb on - strand, within GFF5911 at 126.704 kb on + strand, within GFF5911 at 126.704 kb on + strand, within GFF5911
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 123,853 + GFF5907 0.74 -0.0 123,853 + GFF5907 0.74 -0.6 124,112 + -0.3 124,114 + -0.4 124,114 + -0.3 124,115 - -0.3 124,180 - -0.1 124,195 - -2.2 124,877 + GFF5909 0.18 +1.6 124,944 - GFF5909 0.25 -0.5 124,976 + GFF5909 0.28 -0.7 124,976 + GFF5909 0.28 -0.8 124,976 + GFF5909 0.28 +1.0 124,977 - GFF5909 0.28 -0.7 124,977 - GFF5909 0.28 -0.3 124,977 - GFF5909 0.28 +1.0 125,235 - GFF5909 0.53 +2.0 125,798 - GFF5910 0.36 +0.2 126,048 - +1.6 126,407 + GFF5911 0.30 -0.3 126,516 - GFF5911 0.38 -0.9 126,516 - GFF5911 0.38 -0.8 126,659 + GFF5911 0.49 +0.9 126,660 - GFF5911 0.49 +0.8 126,701 + GFF5911 0.52 +0.4 126,702 - GFF5911 0.52 +2.7 126,702 - GFF5911 0.52 +0.5 126,702 - GFF5911 0.52 -0.1 126,704 + GFF5911 0.53 -1.4 126,704 + GFF5911 0.53 +0.6
Or see this region's nucleotide sequence