Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF57 and GFF58 are separated by 93 nucleotides GFF58 and GFF59 overlap by 4 nucleotides GFF59 and GFF60 are separated by 66 nucleotides
GFF57 - Putative polysaccharide export protein YccZ precursor, at 71,661 to 72,872
GFF57
GFF58 - Tyrosine-protein kinase (EC 2.7.10.2), at 72,966 to 75,230
GFF58
GFF59 - Undecaprenyl-phosphate galactosephosphotransferase (EC 2.7.8.6), at 75,227 to 75,928
GFF59
GFF60 - GDP-L-fucose synthetase (EC 1.1.1.271), at 75,995 to 76,942
GFF60
Position (kb) 72
73
74
75
76 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 71.983 kb on - strand, within GFF57 at 71.983 kb on - strand, within GFF57 at 72.022 kb on - strand, within GFF57 at 72.022 kb on - strand, within GFF57 at 72.022 kb on - strand, within GFF57 at 72.141 kb on + strand, within GFF57 at 72.274 kb on - strand, within GFF57 at 72.525 kb on + strand, within GFF57 at 72.526 kb on - strand, within GFF57 at 72.526 kb on - strand, within GFF57 at 72.651 kb on + strand, within GFF57 at 72.651 kb on + strand, within GFF57 at 72.651 kb on + strand, within GFF57 at 72.651 kb on + strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.771 kb on + strand at 72.771 kb on + strand at 72.771 kb on + strand at 72.771 kb on + strand at 72.772 kb on - strand at 72.772 kb on - strand at 73.084 kb on - strand at 73.278 kb on + strand, within GFF58 at 73.278 kb on + strand, within GFF58 at 73.279 kb on - strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.378 kb on - strand, within GFF58 at 73.476 kb on + strand, within GFF58 at 73.476 kb on + strand, within GFF58 at 73.477 kb on - strand, within GFF58 at 73.477 kb on - strand, within GFF58 at 73.620 kb on + strand, within GFF58 at 73.621 kb on - strand, within GFF58 at 73.621 kb on - strand, within GFF58 at 73.764 kb on + strand, within GFF58 at 73.764 kb on + strand, within GFF58 at 73.764 kb on + strand, within GFF58 at 73.765 kb on - strand, within GFF58 at 73.765 kb on - strand, within GFF58 at 73.765 kb on - strand, within GFF58 at 73.875 kb on + strand, within GFF58 at 73.875 kb on + strand, within GFF58 at 73.875 kb on + strand, within GFF58 at 73.875 kb on + strand, within GFF58 at 73.875 kb on + strand, within GFF58 at 73.875 kb on + strand, within GFF58 at 73.875 kb on + strand, within GFF58 at 73.875 kb on + strand, within GFF58 at 73.875 kb on + strand, within GFF58 at 73.876 kb on - strand, within GFF58 at 73.876 kb on - strand, within GFF58 at 73.876 kb on - strand, within GFF58 at 73.876 kb on - strand, within GFF58 at 73.885 kb on - strand, within GFF58 at 74.146 kb on - strand, within GFF58 at 74.409 kb on + strand, within GFF58 at 74.409 kb on + strand, within GFF58 at 74.410 kb on - strand, within GFF58 at 74.410 kb on - strand, within GFF58 at 74.791 kb on - strand, within GFF58 at 74.934 kb on - strand, within GFF58 at 75.121 kb on - strand at 75.127 kb on - strand at 75.136 kb on - strand at 75.294 kb on - strand at 75.390 kb on - strand, within GFF59 at 75.399 kb on + strand, within GFF59 at 75.440 kb on + strand, within GFF59 at 75.441 kb on - strand, within GFF59 at 75.441 kb on - strand, within GFF59 at 75.441 kb on - strand, within GFF59 at 75.441 kb on - strand, within GFF59 at 75.441 kb on - strand, within GFF59 at 75.441 kb on - strand, within GFF59 at 75.441 kb on - strand, within GFF59 at 75.441 kb on - strand, within GFF59 at 75.575 kb on + strand, within GFF59 at 75.576 kb on - strand, within GFF59 at 75.632 kb on + strand, within GFF59 at 75.633 kb on - strand, within GFF59 at 75.633 kb on - strand, within GFF59 at 75.633 kb on - strand, within GFF59 at 75.633 kb on - strand, within GFF59 at 75.728 kb on + strand, within GFF59 at 75.737 kb on + strand, within GFF59 at 75.738 kb on - strand, within GFF59 at 75.738 kb on - strand, within GFF59 at 75.750 kb on - strand, within GFF59 at 75.812 kb on + strand, within GFF59 at 75.813 kb on - strand, within GFF59 at 75.843 kb on - strand, within GFF59 at 75.843 kb on - strand, within GFF59 at 75.965 kb on - strand at 76.130 kb on + strand, within GFF60 at 76.130 kb on + strand, within GFF60 at 76.130 kb on + strand, within GFF60 at 76.131 kb on - strand, within GFF60 at 76.131 kb on - strand, within GFF60 at 76.175 kb on + strand, within GFF60 at 76.176 kb on - strand, within GFF60 at 76.193 kb on + strand, within GFF60 at 76.194 kb on - strand, within GFF60
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 71,983 - GFF57 0.27 +0.1 71,983 - GFF57 0.27 +0.3 72,022 - GFF57 0.30 -1.2 72,022 - GFF57 0.30 -0.6 72,022 - GFF57 0.30 +0.5 72,141 + GFF57 0.40 -0.1 72,274 - GFF57 0.51 -1.5 72,525 + GFF57 0.71 -0.4 72,526 - GFF57 0.71 -0.7 72,526 - GFF57 0.71 +1.2 72,651 + GFF57 0.82 +0.2 72,651 + GFF57 0.82 +0.0 72,651 + GFF57 0.82 -1.4 72,651 + GFF57 0.82 -0.0 72,652 - GFF57 0.82 +2.0 72,652 - GFF57 0.82 -0.5 72,652 - GFF57 0.82 -0.8 72,652 - GFF57 0.82 +0.2 72,652 - GFF57 0.82 -1.2 72,771 + +0.4 72,771 + -0.4 72,771 + +0.8 72,771 + -0.2 72,772 - +0.4 72,772 - -1.1 73,084 - -0.8 73,278 + GFF58 0.14 +0.9 73,278 + GFF58 0.14 -1.0 73,279 - GFF58 0.14 -0.3 73,299 + GFF58 0.15 -1.0 73,299 + GFF58 0.15 -1.0 73,299 + GFF58 0.15 -1.1 73,299 + GFF58 0.15 -0.4 73,299 + GFF58 0.15 -0.1 73,299 + GFF58 0.15 -0.8 73,300 - GFF58 0.15 -2.5 73,300 - GFF58 0.15 -0.4 73,300 - GFF58 0.15 -0.2 73,300 - GFF58 0.15 -0.4 73,300 - GFF58 0.15 -0.2 73,378 - GFF58 0.18 +0.1 73,476 + GFF58 0.23 +0.4 73,476 + GFF58 0.23 -0.7 73,477 - GFF58 0.23 +0.0 73,477 - GFF58 0.23 -3.1 73,620 + GFF58 0.29 +0.0 73,621 - GFF58 0.29 -1.0 73,621 - GFF58 0.29 +0.1 73,764 + GFF58 0.35 -0.6 73,764 + GFF58 0.35 -0.9 73,764 + GFF58 0.35 -0.1 73,765 - GFF58 0.35 -0.4 73,765 - GFF58 0.35 +0.7 73,765 - GFF58 0.35 -1.9 73,875 + GFF58 0.40 +1.7 73,875 + GFF58 0.40 +0.8 73,875 + GFF58 0.40 -0.6 73,875 + GFF58 0.40 -0.5 73,875 + GFF58 0.40 +0.0 73,875 + GFF58 0.40 -0.6 73,875 + GFF58 0.40 -0.4 73,875 + GFF58 0.40 -2.1 73,875 + GFF58 0.40 +0.6 73,876 - GFF58 0.40 +0.0 73,876 - GFF58 0.40 -0.8 73,876 - GFF58 0.40 +1.1 73,876 - GFF58 0.40 -0.6 73,885 - GFF58 0.41 +0.7 74,146 - GFF58 0.52 -0.6 74,409 + GFF58 0.64 -0.6 74,409 + GFF58 0.64 +0.2 74,410 - GFF58 0.64 -0.8 74,410 - GFF58 0.64 -0.8 74,791 - GFF58 0.81 +0.2 74,934 - GFF58 0.87 -0.0 75,121 - -0.3 75,127 - +0.4 75,136 - -1.5 75,294 - -1.2 75,390 - GFF59 0.23 +0.4 75,399 + GFF59 0.25 -0.9 75,440 + GFF59 0.30 +0.5 75,441 - GFF59 0.30 +0.4 75,441 - GFF59 0.30 -0.2 75,441 - GFF59 0.30 -1.8 75,441 - GFF59 0.30 -0.2 75,441 - GFF59 0.30 -0.6 75,441 - GFF59 0.30 -0.4 75,441 - GFF59 0.30 +0.5 75,441 - GFF59 0.30 -0.7 75,575 + GFF59 0.50 +0.2 75,576 - GFF59 0.50 +0.2 75,632 + GFF59 0.58 +0.2 75,633 - GFF59 0.58 -0.2 75,633 - GFF59 0.58 -0.1 75,633 - GFF59 0.58 -0.6 75,633 - GFF59 0.58 -1.8 75,728 + GFF59 0.71 +0.2 75,737 + GFF59 0.73 -0.4 75,738 - GFF59 0.73 -0.6 75,738 - GFF59 0.73 -0.6 75,750 - GFF59 0.75 +0.3 75,812 + GFF59 0.83 +2.4 75,813 - GFF59 0.83 +0.2 75,843 - GFF59 0.88 +0.1 75,843 - GFF59 0.88 -1.1 75,965 - -0.0 76,130 + GFF60 0.14 -1.1 76,130 + GFF60 0.14 +0.0 76,130 + GFF60 0.14 -0.2 76,131 - GFF60 0.14 +2.3 76,131 - GFF60 0.14 -0.0 76,175 + GFF60 0.19 -0.5 76,176 - GFF60 0.19 -1.1 76,193 + GFF60 0.21 +1.1 76,194 - GFF60 0.21 +0.0
Or see this region's nucleotide sequence