Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5655 and GFF5656 are separated by 10 nucleotides GFF5656 and GFF5657 are separated by 13 nucleotides GFF5657 and GFF5658 are separated by 1 nucleotides GFF5658 and GFF5659 are separated by 1 nucleotides
GFF5655 - Formate dehydrogenase formation protein FdhE, at 31,553 to 32,581
GFF5655
GFF5656 - Formate dehydrogenase O gamma subunit (EC 1.2.1.2), at 32,592 to 33,215
GFF5656
GFF5657 - Formate dehydrogenase O beta subunit (EC 1.2.1.2), at 33,229 to 34,191
GFF5657
GFF5658 - no description, at 34,193 to 34,354
GFF5658
GFF5659 - Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 34,356 to 36,797
GFF5659
Position (kb)
33
34
35 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 32.294 kb on + strand, within GFF5655 at 32.294 kb on + strand, within GFF5655 at 32.294 kb on + strand, within GFF5655 at 32.295 kb on - strand, within GFF5655 at 32.295 kb on - strand, within GFF5655 at 32.347 kb on - strand, within GFF5655 at 32.347 kb on - strand, within GFF5655 at 32.347 kb on - strand, within GFF5655 at 32.401 kb on - strand, within GFF5655 at 32.401 kb on - strand, within GFF5655 at 32.401 kb on - strand, within GFF5655 at 32.401 kb on - strand, within GFF5655 at 32.590 kb on + strand at 32.590 kb on + strand at 32.591 kb on - strand at 32.591 kb on - strand at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.708 kb on - strand, within GFF5656 at 32.708 kb on - strand, within GFF5656 at 32.708 kb on - strand, within GFF5656 at 32.784 kb on + strand, within GFF5656 at 32.893 kb on + strand, within GFF5656 at 32.894 kb on - strand, within GFF5656 at 32.894 kb on - strand, within GFF5656 at 32.894 kb on - strand, within GFF5656 at 32.894 kb on - strand, within GFF5656 at 33.202 kb on + strand at 33.202 kb on + strand at 33.202 kb on + strand at 33.202 kb on + strand at 33.203 kb on - strand at 33.205 kb on + strand at 33.205 kb on + strand at 33.205 kb on + strand at 33.206 kb on - strand at 33.206 kb on - strand at 33.206 kb on - strand at 33.340 kb on + strand, within GFF5657 at 33.359 kb on + strand, within GFF5657 at 33.359 kb on + strand, within GFF5657 at 33.360 kb on - strand, within GFF5657 at 33.360 kb on - strand, within GFF5657 at 33.476 kb on + strand, within GFF5657 at 33.476 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.510 kb on - strand, within GFF5657 at 33.510 kb on - strand, within GFF5657 at 33.510 kb on - strand, within GFF5657 at 33.510 kb on - strand, within GFF5657 at 33.510 kb on - strand, within GFF5657 at 33.510 kb on - strand, within GFF5657 at 33.545 kb on + strand, within GFF5657 at 33.546 kb on - strand, within GFF5657 at 33.546 kb on - strand, within GFF5657 at 33.546 kb on - strand, within GFF5657 at 33.546 kb on - strand, within GFF5657 at 33.602 kb on + strand, within GFF5657 at 33.603 kb on - strand, within GFF5657 at 33.713 kb on + strand, within GFF5657 at 33.714 kb on - strand, within GFF5657 at 33.714 kb on - strand, within GFF5657 at 33.714 kb on - strand, within GFF5657 at 33.714 kb on - strand, within GFF5657 at 33.714 kb on - strand, within GFF5657 at 33.714 kb on - strand, within GFF5657 at 33.714 kb on - strand, within GFF5657 at 33.824 kb on + strand, within GFF5657 at 33.825 kb on - strand, within GFF5657 at 33.825 kb on - strand, within GFF5657 at 33.825 kb on - strand, within GFF5657 at 33.992 kb on + strand, within GFF5657 at 33.992 kb on + strand, within GFF5657 at 33.992 kb on + strand, within GFF5657 at 33.992 kb on + strand, within GFF5657 at 34.126 kb on + strand at 34.127 kb on - strand at 34.171 kb on + strand at 34.390 kb on + strand at 34.390 kb on + strand at 34.390 kb on + strand at 34.390 kb on + strand at 34.390 kb on + strand at 34.390 kb on + strand at 34.391 kb on - strand at 34.391 kb on - strand at 34.567 kb on + strand at 34.568 kb on - strand at 34.568 kb on - strand at 34.568 kb on - strand at 34.696 kb on + strand, within GFF5659 at 34.697 kb on - strand, within GFF5659 at 34.697 kb on - strand, within GFF5659 at 34.697 kb on - strand, within GFF5659 at 34.697 kb on - strand, within GFF5659 at 34.720 kb on + strand, within GFF5659 at 34.735 kb on + strand, within GFF5659 at 34.735 kb on + strand, within GFF5659 at 34.864 kb on + strand, within GFF5659 at 34.864 kb on + strand, within GFF5659 at 34.865 kb on - strand, within GFF5659 at 34.865 kb on - strand, within GFF5659 at 34.865 kb on - strand, within GFF5659 at 34.865 kb on - strand, within GFF5659 at 35.090 kb on - strand, within GFF5659 at 35.090 kb on - strand, within GFF5659
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 32,294 + GFF5655 0.72 -1.9 32,294 + GFF5655 0.72 -0.1 32,294 + GFF5655 0.72 -1.7 32,295 - GFF5655 0.72 +0.4 32,295 - GFF5655 0.72 -0.7 32,347 - GFF5655 0.77 -0.2 32,347 - GFF5655 0.77 -0.5 32,347 - GFF5655 0.77 -0.7 32,401 - GFF5655 0.82 -0.7 32,401 - GFF5655 0.82 +0.3 32,401 - GFF5655 0.82 +0.9 32,401 - GFF5655 0.82 -0.5 32,590 + +0.4 32,590 + -1.0 32,591 - -0.2 32,591 - +0.8 32,707 + GFF5656 0.18 +0.0 32,707 + GFF5656 0.18 +1.0 32,707 + GFF5656 0.18 +0.5 32,707 + GFF5656 0.18 -0.2 32,707 + GFF5656 0.18 -0.5 32,707 + GFF5656 0.18 +0.3 32,707 + GFF5656 0.18 +1.2 32,707 + GFF5656 0.18 +0.3 32,707 + GFF5656 0.18 +0.1 32,708 - GFF5656 0.19 -0.7 32,708 - GFF5656 0.19 -0.9 32,708 - GFF5656 0.19 +0.9 32,784 + GFF5656 0.31 -0.7 32,893 + GFF5656 0.48 -0.5 32,894 - GFF5656 0.48 +0.6 32,894 - GFF5656 0.48 +0.2 32,894 - GFF5656 0.48 -0.3 32,894 - GFF5656 0.48 +0.2 33,202 + +1.4 33,202 + -1.1 33,202 + -0.4 33,202 + -1.0 33,203 - +1.1 33,205 + -0.7 33,205 + -1.6 33,205 + -0.8 33,206 - -0.2 33,206 - +1.4 33,206 - -1.4 33,340 + GFF5657 0.12 -0.8 33,359 + GFF5657 0.13 +0.2 33,359 + GFF5657 0.13 -0.3 33,360 - GFF5657 0.14 +0.4 33,360 - GFF5657 0.14 -0.7 33,476 + GFF5657 0.26 +0.4 33,476 + GFF5657 0.26 -0.2 33,509 + GFF5657 0.29 -0.9 33,509 + GFF5657 0.29 +0.0 33,509 + GFF5657 0.29 -0.7 33,509 + GFF5657 0.29 -0.2 33,509 + GFF5657 0.29 +0.2 33,509 + GFF5657 0.29 -1.5 33,509 + GFF5657 0.29 -1.1 33,509 + GFF5657 0.29 -2.9 33,509 + GFF5657 0.29 -0.2 33,509 + GFF5657 0.29 -0.8 33,510 - GFF5657 0.29 +0.2 33,510 - GFF5657 0.29 -0.3 33,510 - GFF5657 0.29 -0.8 33,510 - GFF5657 0.29 -1.3 33,510 - GFF5657 0.29 -0.3 33,510 - GFF5657 0.29 -0.5 33,545 + GFF5657 0.33 -1.5 33,546 - GFF5657 0.33 +0.4 33,546 - GFF5657 0.33 +0.3 33,546 - GFF5657 0.33 -0.6 33,546 - GFF5657 0.33 -0.4 33,602 + GFF5657 0.39 -0.9 33,603 - GFF5657 0.39 +2.6 33,713 + GFF5657 0.50 -0.2 33,714 - GFF5657 0.50 -0.1 33,714 - GFF5657 0.50 -0.3 33,714 - GFF5657 0.50 +0.2 33,714 - GFF5657 0.50 -0.7 33,714 - GFF5657 0.50 -0.3 33,714 - GFF5657 0.50 -2.2 33,714 - GFF5657 0.50 -1.4 33,824 + GFF5657 0.62 -2.2 33,825 - GFF5657 0.62 +0.4 33,825 - GFF5657 0.62 +0.2 33,825 - GFF5657 0.62 -0.4 33,992 + GFF5657 0.79 -0.5 33,992 + GFF5657 0.79 +0.8 33,992 + GFF5657 0.79 -0.3 33,992 + GFF5657 0.79 -0.9 34,126 + -0.9 34,127 - -0.3 34,171 + -0.5 34,390 + -0.2 34,390 + -0.1 34,390 + -0.0 34,390 + +0.3 34,390 + -1.9 34,390 + -0.5 34,391 - -0.1 34,391 - -0.1 34,567 + -0.2 34,568 - -0.6 34,568 - +0.0 34,568 - +0.1 34,696 + GFF5659 0.14 -0.0 34,697 - GFF5659 0.14 +0.1 34,697 - GFF5659 0.14 -0.2 34,697 - GFF5659 0.14 +1.2 34,697 - GFF5659 0.14 -0.1 34,720 + GFF5659 0.15 -0.5 34,735 + GFF5659 0.16 +0.2 34,735 + GFF5659 0.16 -0.8 34,864 + GFF5659 0.21 -1.4 34,864 + GFF5659 0.21 +0.2 34,865 - GFF5659 0.21 +0.4 34,865 - GFF5659 0.21 +2.2 34,865 - GFF5659 0.21 +0.5 34,865 - GFF5659 0.21 -0.5 35,090 - GFF5659 0.30 -0.6 35,090 - GFF5659 0.30 -0.9
Or see this region's nucleotide sequence