Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4967 and GFF4968 are separated by 19 nucleotides GFF4968 and GFF4969 overlap by 1 nucleotides GFF4969 and GFF4970 are separated by 24 nucleotides
GFF4967 - Glutathione S-transferase family protein, at 67,867 to 68,475
GFF4967
GFF4968 - Succinate dehydrogenase/fumarate reductase, flavoprotein subunit, at 68,495 to 70,219
GFF4968
GFF4969 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 70,219 to 70,962
GFF4969
GFF4970 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 70,987 to 71,736
GFF4970
Position (kb) 68
69
70
71 Strain fitness (log2 ratio) -2
-1
0
1
2 at 67.512 kb on + strand at 67.512 kb on + strand at 67.512 kb on + strand at 67.512 kb on + strand at 67.512 kb on + strand at 67.648 kb on + strand at 67.649 kb on - strand at 67.649 kb on - strand at 67.818 kb on - strand at 68.036 kb on + strand, within GFF4967 at 68.631 kb on + strand at 68.631 kb on + strand at 68.631 kb on + strand at 68.632 kb on - strand at 68.632 kb on - strand at 68.737 kb on - strand, within GFF4968 at 68.737 kb on - strand, within GFF4968 at 68.809 kb on - strand, within GFF4968 at 68.943 kb on + strand, within GFF4968 at 68.944 kb on - strand, within GFF4968 at 68.949 kb on + strand, within GFF4968 at 68.949 kb on + strand, within GFF4968 at 69.078 kb on + strand, within GFF4968 at 69.079 kb on - strand, within GFF4968 at 69.103 kb on - strand, within GFF4968 at 69.354 kb on + strand, within GFF4968 at 69.843 kb on + strand, within GFF4968 at 69.847 kb on - strand, within GFF4968 at 69.847 kb on - strand, within GFF4968 at 69.858 kb on + strand, within GFF4968 at 69.859 kb on - strand, within GFF4968 at 69.900 kb on + strand, within GFF4968 at 69.982 kb on - strand, within GFF4968 at 70.484 kb on + strand, within GFF4969 at 70.514 kb on + strand, within GFF4969 at 70.514 kb on + strand, within GFF4969 at 70.514 kb on + strand, within GFF4969 at 70.515 kb on - strand, within GFF4969 at 70.515 kb on - strand, within GFF4969 at 70.637 kb on + strand, within GFF4969 at 70.637 kb on + strand, within GFF4969 at 70.637 kb on + strand, within GFF4969 at 70.844 kb on + strand, within GFF4969 at 70.844 kb on + strand, within GFF4969 at 71.036 kb on + strand at 71.036 kb on + strand at 71.036 kb on + strand at 71.036 kb on + strand at 71.036 kb on + strand at 71.037 kb on - strand at 71.037 kb on - strand at 71.037 kb on - strand at 71.040 kb on + strand at 71.041 kb on - strand at 71.041 kb on - strand at 71.041 kb on - strand at 71.121 kb on + strand, within GFF4970 at 71.121 kb on + strand, within GFF4970 at 71.121 kb on + strand, within GFF4970 at 71.122 kb on - strand, within GFF4970 at 71.122 kb on - strand, within GFF4970 at 71.122 kb on - strand, within GFF4970 at 71.123 kb on + strand, within GFF4970 at 71.123 kb on + strand, within GFF4970 at 71.123 kb on + strand, within GFF4970 at 71.123 kb on + strand, within GFF4970 at 71.124 kb on - strand, within GFF4970 at 71.124 kb on - strand, within GFF4970
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 67,512 + -1.2 67,512 + -1.0 67,512 + -0.6 67,512 + -0.5 67,512 + +2.2 67,648 + -0.1 67,649 - -0.1 67,649 - +0.1 67,818 - +0.4 68,036 + GFF4967 0.28 -0.4 68,631 + -0.0 68,631 + -0.5 68,631 + +0.2 68,632 - +0.2 68,632 - -0.0 68,737 - GFF4968 0.14 +0.6 68,737 - GFF4968 0.14 +0.8 68,809 - GFF4968 0.18 -0.3 68,943 + GFF4968 0.26 -0.8 68,944 - GFF4968 0.26 -0.4 68,949 + GFF4968 0.26 -0.8 68,949 + GFF4968 0.26 -0.4 69,078 + GFF4968 0.34 +0.9 69,079 - GFF4968 0.34 -0.2 69,103 - GFF4968 0.35 -2.3 69,354 + GFF4968 0.50 +0.5 69,843 + GFF4968 0.78 -0.6 69,847 - GFF4968 0.78 -1.9 69,847 - GFF4968 0.78 +0.8 69,858 + GFF4968 0.79 +0.4 69,859 - GFF4968 0.79 -1.1 69,900 + GFF4968 0.81 -0.6 69,982 - GFF4968 0.86 -0.8 70,484 + GFF4969 0.36 +1.3 70,514 + GFF4969 0.40 +0.1 70,514 + GFF4969 0.40 -0.0 70,514 + GFF4969 0.40 -0.1 70,515 - GFF4969 0.40 -0.8 70,515 - GFF4969 0.40 -0.6 70,637 + GFF4969 0.56 +0.8 70,637 + GFF4969 0.56 -0.8 70,637 + GFF4969 0.56 -0.1 70,844 + GFF4969 0.84 -1.4 70,844 + GFF4969 0.84 +1.2 71,036 + -0.3 71,036 + -0.7 71,036 + +0.1 71,036 + -0.8 71,036 + -0.2 71,037 - -0.5 71,037 - -0.5 71,037 - +1.1 71,040 + +0.2 71,041 - -0.3 71,041 - -0.5 71,041 - -0.3 71,121 + GFF4970 0.18 +1.3 71,121 + GFF4970 0.18 +0.4 71,121 + GFF4970 0.18 -0.6 71,122 - GFF4970 0.18 -0.6 71,122 - GFF4970 0.18 +0.6 71,122 - GFF4970 0.18 -0.7 71,123 + GFF4970 0.18 -0.2 71,123 + GFF4970 0.18 +0.8 71,123 + GFF4970 0.18 -2.4 71,123 + GFF4970 0.18 +0.6 71,124 - GFF4970 0.18 +0.9 71,124 - GFF4970 0.18 -1.0
Or see this region's nucleotide sequence