Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4134 and GFF4135 overlap by 4 nucleotides GFF4135 and GFF4136 are separated by 16 nucleotides
GFF4134 - Small molecule metabolism, at 40,835 to 41,869
GFF4134
GFF4135 - Acyl-CoA dehydrogenase; probable dibenzothiophene desulfurization enzyme, at 41,866 to 43,104
GFF4135
GFF4136 - hypothetical protein, at 43,121 to 46,051
GFF4136
Position (kb) 41
42
43
44 Strain fitness (log2 ratio) -2
-1
0
1
2 at 41.001 kb on - strand, within GFF4134 at 41.001 kb on - strand, within GFF4134 at 41.110 kb on + strand, within GFF4134 at 41.198 kb on + strand, within GFF4134 at 41.198 kb on + strand, within GFF4134 at 41.285 kb on + strand, within GFF4134 at 41.381 kb on + strand, within GFF4134 at 41.382 kb on - strand, within GFF4134 at 41.382 kb on - strand, within GFF4134 at 41.433 kb on - strand, within GFF4134 at 41.436 kb on - strand, within GFF4134 at 41.436 kb on - strand, within GFF4134 at 41.456 kb on + strand, within GFF4134 at 41.456 kb on + strand, within GFF4134 at 41.720 kb on + strand, within GFF4134 at 41.721 kb on - strand, within GFF4134 at 41.739 kb on - strand, within GFF4134 at 42.121 kb on + strand, within GFF4135 at 42.122 kb on - strand, within GFF4135 at 42.379 kb on + strand, within GFF4135 at 42.379 kb on + strand, within GFF4135 at 42.379 kb on + strand, within GFF4135 at 42.380 kb on - strand, within GFF4135 at 42.406 kb on + strand, within GFF4135 at 42.406 kb on + strand, within GFF4135 at 42.567 kb on - strand, within GFF4135 at 42.593 kb on - strand, within GFF4135 at 43.224 kb on + strand at 43.225 kb on - strand at 43.368 kb on + strand at 43.462 kb on - strand, within GFF4136 at 43.515 kb on + strand, within GFF4136 at 43.516 kb on - strand, within GFF4136 at 43.630 kb on - strand, within GFF4136 at 43.900 kb on - strand, within GFF4136
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 41,001 - GFF4134 0.16 +1.3 41,001 - GFF4134 0.16 +0.8 41,110 + GFF4134 0.27 +0.0 41,198 + GFF4134 0.35 +0.8 41,198 + GFF4134 0.35 -0.3 41,285 + GFF4134 0.43 -0.1 41,381 + GFF4134 0.53 -0.2 41,382 - GFF4134 0.53 -0.5 41,382 - GFF4134 0.53 +1.1 41,433 - GFF4134 0.58 +0.2 41,436 - GFF4134 0.58 -0.8 41,436 - GFF4134 0.58 -0.2 41,456 + GFF4134 0.60 +2.4 41,456 + GFF4134 0.60 +1.3 41,720 + GFF4134 0.86 +1.1 41,721 - GFF4134 0.86 -1.6 41,739 - GFF4134 0.87 -1.8 42,121 + GFF4135 0.21 -0.3 42,122 - GFF4135 0.21 +0.9 42,379 + GFF4135 0.41 -0.2 42,379 + GFF4135 0.41 +0.5 42,379 + GFF4135 0.41 +0.1 42,380 - GFF4135 0.41 -1.8 42,406 + GFF4135 0.44 +1.7 42,406 + GFF4135 0.44 -0.6 42,567 - GFF4135 0.57 +0.5 42,593 - GFF4135 0.59 -0.3 43,224 + +0.3 43,225 - +1.1 43,368 + -0.7 43,462 - GFF4136 0.12 +0.2 43,515 + GFF4136 0.13 +0.1 43,516 - GFF4136 0.13 +0.6 43,630 - GFF4136 0.17 -0.2 43,900 - GFF4136 0.27 -0.7
Or see this region's nucleotide sequence