Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4038 and GFF4039 overlap by 4 nucleotides GFF4039 and GFF4040 are separated by 15 nucleotides GFF4040 and GFF4041 are separated by 749 nucleotides
GFF4038 - Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family, at 247,734 to 248,951
GFF4038
GFF4039 - Heavy metal RND efflux outer membrane protein, CzcC family, at 248,948 to 250,267
GFF4039
GFF4040 - no description, at 250,283 to 250,414
GFF4040
GFF4041 - Lipid A phosphoethanolamine transferase, putative, at 251,164 to 252,579
GFF4041
Position (kb)
248
249
250
251 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 248.101 kb on + strand, within GFF4038 at 248.438 kb on - strand, within GFF4038 at 248.488 kb on + strand, within GFF4038 at 248.489 kb on - strand, within GFF4038 at 248.880 kb on - strand at 249.488 kb on - strand, within GFF4039 at 249.804 kb on + strand, within GFF4039 at 249.804 kb on + strand, within GFF4039 at 249.805 kb on - strand, within GFF4039 at 250.027 kb on - strand, within GFF4039 at 250.027 kb on - strand, within GFF4039 at 250.279 kb on - strand at 250.412 kb on + strand at 250.412 kb on + strand at 250.413 kb on - strand at 250.413 kb on - strand at 250.413 kb on - strand at 250.416 kb on + strand at 250.416 kb on + strand at 250.417 kb on - strand at 250.417 kb on - strand at 250.447 kb on - strand at 250.638 kb on - strand at 250.693 kb on + strand at 250.704 kb on + strand at 250.704 kb on + strand at 250.704 kb on + strand at 250.705 kb on - strand at 250.705 kb on - strand at 250.705 kb on - strand at 250.705 kb on - strand at 250.705 kb on - strand at 250.705 kb on - strand at 250.735 kb on - strand at 250.740 kb on + strand at 250.803 kb on - strand at 250.803 kb on - strand at 251.227 kb on + strand at 251.228 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 248,101 + GFF4038 0.30 -0.2 248,438 - GFF4038 0.58 -0.3 248,488 + GFF4038 0.62 -1.2 248,489 - GFF4038 0.62 -0.7 248,880 - -0.1 249,488 - GFF4039 0.41 +1.0 249,804 + GFF4039 0.65 +3.1 249,804 + GFF4039 0.65 -1.8 249,805 - GFF4039 0.65 -0.3 250,027 - GFF4039 0.82 +0.1 250,027 - GFF4039 0.82 -2.1 250,279 - -1.0 250,412 + +0.1 250,412 + -1.1 250,413 - -0.7 250,413 - +0.0 250,413 - +0.6 250,416 + +0.1 250,416 + -0.2 250,417 - -1.2 250,417 - -1.2 250,447 - +0.1 250,638 - +0.0 250,693 + -0.2 250,704 + +0.3 250,704 + -0.1 250,704 + -0.3 250,705 - -0.7 250,705 - +0.0 250,705 - +0.2 250,705 - -0.1 250,705 - +0.1 250,705 - +0.1 250,735 - -0.3 250,740 + -2.0 250,803 - +0.0 250,803 - +0.5 251,227 + +0.6 251,228 - -0.1
Or see this region's nucleotide sequence