Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3516 and GFF3517 are separated by 13 nucleotides GFF3517 and GFF3518 are separated by 13 nucleotides GFF3518 and GFF3519 overlap by 11 nucleotides GFF3519 and GFF3520 are separated by 14 nucleotides
GFF3516 - Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 20,741 to 22,843
GFF3516
GFF3517 - Potassium-transporting ATPase C chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 22,857 to 23,468
GFF3517
GFF3518 - no description, at 23,482 to 23,856
GFF3518
GFF3519 - hypothetical protein, at 23,846 to 23,977
GFF3519
GFF3520 - Osmosensitive K+ channel histidine kinase KdpD, at 23,992 to 26,745
GFF3520
Position (kb) 23
24 Strain fitness (log2 ratio) -1
0
1 at 22.553 kb on + strand, within GFF3516 at 22.589 kb on + strand, within GFF3516 at 22.589 kb on + strand, within GFF3516 at 22.590 kb on - strand, within GFF3516 at 22.763 kb on + strand at 23.048 kb on - strand, within GFF3517 at 23.100 kb on + strand, within GFF3517 at 23.100 kb on + strand, within GFF3517 at 23.488 kb on + strand at 23.488 kb on + strand at 23.489 kb on - strand at 23.575 kb on + strand, within GFF3518 at 23.575 kb on + strand, within GFF3518 at 23.575 kb on + strand, within GFF3518 at 23.575 kb on + strand, within GFF3518 at 23.575 kb on + strand, within GFF3518 at 23.576 kb on - strand, within GFF3518 at 23.576 kb on - strand, within GFF3518 at 23.747 kb on - strand, within GFF3518 at 23.975 kb on + strand at 23.976 kb on - strand at 24.079 kb on + strand at 24.080 kb on - strand at 24.080 kb on - strand at 24.080 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 22,553 + GFF3516 0.86 -1.7 22,589 + GFF3516 0.88 +0.6 22,589 + GFF3516 0.88 -0.1 22,590 - GFF3516 0.88 -0.3 22,763 + -0.1 23,048 - GFF3517 0.31 +0.7 23,100 + GFF3517 0.40 -0.4 23,100 + GFF3517 0.40 +0.0 23,488 + -0.5 23,488 + +0.8 23,489 - +0.2 23,575 + GFF3518 0.25 +0.6 23,575 + GFF3518 0.25 +1.4 23,575 + GFF3518 0.25 -0.5 23,575 + GFF3518 0.25 -0.2 23,575 + GFF3518 0.25 +0.2 23,576 - GFF3518 0.25 -0.7 23,576 - GFF3518 0.25 +0.2 23,747 - GFF3518 0.71 +0.0 23,975 + +1.0 23,976 - +0.4 24,079 + +0.7 24,080 - -0.6 24,080 - -0.2 24,080 - +0.9
Or see this region's nucleotide sequence