Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2895 and GFF2896 are separated by 146 nucleotides GFF2896 and GFF2897 are separated by 28 nucleotides GFF2897 and GFF2898 are separated by 25 nucleotides
GFF2895 - Vanillate O-demethylase oxygenase subunit (EC 1.14.13.82), at 320,648 to 321,766
GFF2895
GFF2896 - Methionine synthase II (cobalamin-independent), at 321,913 to 323,034
GFF2896
GFF2897 - Glutathione S-transferase-like( EC:2.5.1.18 ), at 323,063 to 323,737
GFF2897
GFF2898 - Cardiolipin synthase, bacterial type ClsA, at 323,763 to 325,145
GFF2898
Position (kb) 321
322
323
324 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 320.931 kb on + strand, within GFF2895 at 320.931 kb on + strand, within GFF2895 at 320.932 kb on - strand, within GFF2895 at 320.932 kb on - strand, within GFF2895 at 320.932 kb on - strand, within GFF2895 at 320.932 kb on - strand, within GFF2895 at 320.976 kb on + strand, within GFF2895 at 321.531 kb on + strand, within GFF2895 at 321.532 kb on - strand, within GFF2895 at 321.532 kb on - strand, within GFF2895 at 321.532 kb on - strand, within GFF2895 at 321.652 kb on - strand, within GFF2895 at 321.652 kb on - strand, within GFF2895 at 321.726 kb on + strand at 321.726 kb on + strand at 321.726 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.838 kb on - strand at 321.843 kb on + strand at 321.871 kb on + strand at 321.872 kb on - strand at 321.976 kb on + strand at 321.976 kb on + strand at 321.976 kb on + strand at 321.976 kb on + strand at 321.976 kb on + strand at 321.977 kb on - strand at 321.977 kb on - strand at 321.977 kb on - strand at 321.977 kb on - strand at 321.977 kb on - strand at 322.126 kb on + strand, within GFF2896 at 322.126 kb on + strand, within GFF2896 at 322.126 kb on + strand, within GFF2896 at 322.126 kb on + strand, within GFF2896 at 322.126 kb on + strand, within GFF2896 at 322.127 kb on - strand, within GFF2896 at 322.127 kb on - strand, within GFF2896 at 322.127 kb on - strand, within GFF2896 at 322.285 kb on + strand, within GFF2896 at 322.285 kb on + strand, within GFF2896 at 322.286 kb on - strand, within GFF2896 at 322.415 kb on - strand, within GFF2896 at 322.435 kb on + strand, within GFF2896 at 322.435 kb on + strand, within GFF2896 at 322.435 kb on + strand, within GFF2896 at 322.435 kb on + strand, within GFF2896 at 322.435 kb on + strand, within GFF2896 at 322.436 kb on - strand, within GFF2896 at 322.477 kb on + strand, within GFF2896 at 322.477 kb on + strand, within GFF2896 at 322.477 kb on + strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.507 kb on + strand, within GFF2896 at 322.507 kb on + strand, within GFF2896 at 322.507 kb on + strand, within GFF2896 at 322.507 kb on + strand, within GFF2896 at 322.508 kb on - strand, within GFF2896 at 322.508 kb on - strand, within GFF2896 at 322.508 kb on - strand, within GFF2896 at 322.508 kb on - strand, within GFF2896 at 322.508 kb on - strand, within GFF2896 at 322.508 kb on - strand, within GFF2896 at 322.687 kb on + strand, within GFF2896 at 322.687 kb on + strand, within GFF2896 at 322.687 kb on + strand, within GFF2896 at 322.687 kb on + strand, within GFF2896 at 322.688 kb on - strand, within GFF2896 at 322.688 kb on - strand, within GFF2896 at 322.688 kb on - strand, within GFF2896 at 322.688 kb on - strand, within GFF2896 at 322.688 kb on - strand, within GFF2896 at 322.688 kb on - strand, within GFF2896 at 322.735 kb on + strand, within GFF2896 at 322.736 kb on - strand, within GFF2896 at 322.736 kb on - strand, within GFF2896 at 322.736 kb on - strand, within GFF2896 at 322.747 kb on + strand, within GFF2896 at 322.748 kb on - strand, within GFF2896 at 322.748 kb on - strand, within GFF2896 at 322.891 kb on + strand, within GFF2896 at 322.891 kb on + strand, within GFF2896 at 322.891 kb on + strand, within GFF2896 at 322.891 kb on + strand, within GFF2896 at 322.892 kb on - strand, within GFF2896 at 322.892 kb on - strand, within GFF2896 at 322.892 kb on - strand, within GFF2896 at 322.892 kb on - strand, within GFF2896 at 322.892 kb on - strand, within GFF2896 at 323.078 kb on + strand at 323.078 kb on + strand at 323.078 kb on + strand at 323.078 kb on + strand at 323.078 kb on + strand at 323.078 kb on + strand at 323.078 kb on + strand at 323.079 kb on - strand at 323.079 kb on - strand at 323.079 kb on - strand at 323.112 kb on + strand at 323.183 kb on + strand, within GFF2897 at 323.184 kb on - strand, within GFF2897 at 323.300 kb on + strand, within GFF2897 at 323.301 kb on - strand, within GFF2897 at 323.483 kb on + strand, within GFF2897 at 323.484 kb on - strand, within GFF2897 at 323.484 kb on - strand, within GFF2897 at 323.486 kb on + strand, within GFF2897 at 323.486 kb on + strand, within GFF2897 at 323.486 kb on + strand, within GFF2897 at 323.486 kb on + strand, within GFF2897 at 323.486 kb on + strand, within GFF2897 at 323.487 kb on - strand, within GFF2897 at 323.522 kb on + strand, within GFF2897 at 323.538 kb on - strand, within GFF2897 at 323.561 kb on + strand, within GFF2897 at 323.561 kb on + strand, within GFF2897 at 323.561 kb on + strand, within GFF2897 at 323.561 kb on + strand, within GFF2897 at 323.562 kb on - strand, within GFF2897 at 323.562 kb on - strand, within GFF2897 at 323.562 kb on - strand, within GFF2897 at 323.562 kb on - strand, within GFF2897 at 323.562 kb on - strand, within GFF2897 at 323.562 kb on - strand, within GFF2897 at 323.562 kb on - strand, within GFF2897 at 323.562 kb on - strand, within GFF2897 at 323.562 kb on - strand, within GFF2897 at 323.562 kb on - strand, within GFF2897 at 323.562 kb on - strand, within GFF2897 at 323.716 kb on - strand at 323.716 kb on - strand at 323.841 kb on + strand at 323.841 kb on + strand at 323.841 kb on + strand at 323.841 kb on + strand at 323.841 kb on + strand at 323.842 kb on - strand at 323.842 kb on - strand at 323.842 kb on - strand at 323.842 kb on - strand at 323.842 kb on - strand at 323.925 kb on + strand, within GFF2898 at 323.925 kb on + strand, within GFF2898 at 323.925 kb on + strand, within GFF2898 at 323.925 kb on + strand, within GFF2898 at 323.926 kb on - strand, within GFF2898 at 323.926 kb on - strand, within GFF2898 at 323.930 kb on - strand, within GFF2898 at 323.943 kb on + strand, within GFF2898 at 323.943 kb on + strand, within GFF2898 at 323.943 kb on + strand, within GFF2898 at 323.944 kb on - strand, within GFF2898 at 323.944 kb on - strand, within GFF2898 at 323.944 kb on - strand, within GFF2898 at 323.944 kb on - strand, within GFF2898
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 320,931 + GFF2895 0.25 -0.5 320,931 + GFF2895 0.25 -1.0 320,932 - GFF2895 0.25 -0.6 320,932 - GFF2895 0.25 -0.8 320,932 - GFF2895 0.25 -1.0 320,932 - GFF2895 0.25 -1.2 320,976 + GFF2895 0.29 -0.2 321,531 + GFF2895 0.79 -0.9 321,532 - GFF2895 0.79 -0.4 321,532 - GFF2895 0.79 -1.0 321,532 - GFF2895 0.79 -1.3 321,652 - GFF2895 0.90 +0.1 321,652 - GFF2895 0.90 -0.6 321,726 + -0.0 321,726 + -0.6 321,726 + -0.4 321,818 + -1.0 321,818 + -0.6 321,818 + -1.2 321,818 + -0.3 321,818 + -1.1 321,818 + +1.9 321,818 + -1.3 321,818 + +1.8 321,818 + +0.3 321,818 + -0.3 321,818 + -0.5 321,818 + -0.9 321,818 + -0.4 321,818 + -1.3 321,818 + +0.4 321,818 + -0.3 321,818 + -2.1 321,818 + -0.2 321,818 + -1.0 321,818 + -1.2 321,818 + -0.4 321,818 + -0.7 321,818 + -1.4 321,818 + +0.0 321,818 + +1.3 321,818 + +0.1 321,818 + +1.2 321,818 + -0.6 321,818 + -0.7 321,818 + +0.0 321,818 + +0.3 321,818 + +0.6 321,818 + -1.2 321,818 + -0.7 321,818 + +0.3 321,818 + -1.0 321,818 + +0.4 321,818 + +1.2 321,818 + -1.6 321,818 + -0.2 321,818 + +0.6 321,818 + +0.6 321,818 + +1.3 321,818 + -0.7 321,818 + -0.9 321,818 + +0.1 321,818 + -1.0 321,818 + -0.8 321,818 + -0.0 321,818 + +0.8 321,818 + -2.0 321,818 + -0.6 321,818 + +0.8 321,818 + -0.3 321,819 - +0.7 321,819 - -0.4 321,819 - -0.4 321,819 - +1.1 321,819 - -0.3 321,819 - +0.3 321,819 - -0.8 321,819 - +0.2 321,819 - +0.2 321,819 - +0.4 321,819 - -3.1 321,819 - -0.6 321,819 - -0.7 321,819 - -1.0 321,819 - +0.2 321,819 - -0.9 321,819 - +1.5 321,819 - +0.6 321,819 - +0.3 321,819 - +0.1 321,819 - -0.2 321,819 - -0.6 321,819 - -0.9 321,819 - +0.5 321,819 - -1.3 321,819 - +0.1 321,819 - -0.5 321,819 - +0.7 321,819 - -0.1 321,819 - +0.0 321,819 - -0.7 321,819 - -0.2 321,819 - -0.0 321,819 - +1.6 321,819 - -1.4 321,819 - -1.1 321,819 - +1.4 321,819 - +0.7 321,819 - +0.3 321,819 - -0.8 321,819 - +0.5 321,819 - -2.3 321,819 - +0.2 321,819 - +0.6 321,819 - -0.2 321,838 - -1.3 321,843 + -0.3 321,871 + -0.6 321,872 - -0.1 321,976 + -0.8 321,976 + +0.1 321,976 + +0.8 321,976 + +0.1 321,976 + -1.3 321,977 - -0.3 321,977 - +0.6 321,977 - -0.4 321,977 - +0.3 321,977 - -0.1 322,126 + GFF2896 0.19 +0.4 322,126 + GFF2896 0.19 +0.8 322,126 + GFF2896 0.19 +0.7 322,126 + GFF2896 0.19 -0.1 322,126 + GFF2896 0.19 -1.7 322,127 - GFF2896 0.19 -1.5 322,127 - GFF2896 0.19 -1.1 322,127 - GFF2896 0.19 +0.1 322,285 + GFF2896 0.33 -1.4 322,285 + GFF2896 0.33 -0.7 322,286 - GFF2896 0.33 -0.8 322,415 - GFF2896 0.45 +0.2 322,435 + GFF2896 0.47 -1.7 322,435 + GFF2896 0.47 -0.0 322,435 + GFF2896 0.47 -0.2 322,435 + GFF2896 0.47 +0.0 322,435 + GFF2896 0.47 +0.0 322,436 - GFF2896 0.47 -0.1 322,477 + GFF2896 0.50 -0.9 322,477 + GFF2896 0.50 -0.1 322,477 + GFF2896 0.50 +0.1 322,478 - GFF2896 0.50 -0.1 322,478 - GFF2896 0.50 +0.0 322,478 - GFF2896 0.50 -0.6 322,478 - GFF2896 0.50 -0.4 322,478 - GFF2896 0.50 -0.2 322,478 - GFF2896 0.50 -1.2 322,478 - GFF2896 0.50 -1.0 322,507 + GFF2896 0.53 +1.2 322,507 + GFF2896 0.53 -1.2 322,507 + GFF2896 0.53 -1.1 322,507 + GFF2896 0.53 -0.6 322,508 - GFF2896 0.53 -1.4 322,508 - GFF2896 0.53 -1.6 322,508 - GFF2896 0.53 -0.1 322,508 - GFF2896 0.53 -0.6 322,508 - GFF2896 0.53 +0.1 322,508 - GFF2896 0.53 -1.2 322,687 + GFF2896 0.69 +0.2 322,687 + GFF2896 0.69 -0.6 322,687 + GFF2896 0.69 -0.2 322,687 + GFF2896 0.69 -0.6 322,688 - GFF2896 0.69 -1.0 322,688 - GFF2896 0.69 -0.9 322,688 - GFF2896 0.69 -0.1 322,688 - GFF2896 0.69 -1.7 322,688 - GFF2896 0.69 +1.0 322,688 - GFF2896 0.69 -0.2 322,735 + GFF2896 0.73 +0.0 322,736 - GFF2896 0.73 -0.6 322,736 - GFF2896 0.73 -1.0 322,736 - GFF2896 0.73 -0.5 322,747 + GFF2896 0.74 +0.2 322,748 - GFF2896 0.74 -0.3 322,748 - GFF2896 0.74 -0.1 322,891 + GFF2896 0.87 -1.4 322,891 + GFF2896 0.87 +0.8 322,891 + GFF2896 0.87 +2.2 322,891 + GFF2896 0.87 -1.3 322,892 - GFF2896 0.87 -0.3 322,892 - GFF2896 0.87 -0.5 322,892 - GFF2896 0.87 -1.1 322,892 - GFF2896 0.87 -2.4 322,892 - GFF2896 0.87 +0.6 323,078 + -0.2 323,078 + -0.3 323,078 + -0.1 323,078 + +0.5 323,078 + -1.4 323,078 + -0.3 323,078 + -0.3 323,079 - +0.3 323,079 - -0.1 323,079 - +0.4 323,112 + +0.1 323,183 + GFF2897 0.18 -0.1 323,184 - GFF2897 0.18 -0.4 323,300 + GFF2897 0.35 +0.9 323,301 - GFF2897 0.35 -0.2 323,483 + GFF2897 0.62 +0.0 323,484 - GFF2897 0.62 +0.3 323,484 - GFF2897 0.62 -1.3 323,486 + GFF2897 0.63 -0.1 323,486 + GFF2897 0.63 -1.5 323,486 + GFF2897 0.63 -1.0 323,486 + GFF2897 0.63 -0.2 323,486 + GFF2897 0.63 +0.1 323,487 - GFF2897 0.63 -0.9 323,522 + GFF2897 0.68 +0.5 323,538 - GFF2897 0.70 -0.4 323,561 + GFF2897 0.74 -0.3 323,561 + GFF2897 0.74 +1.6 323,561 + GFF2897 0.74 -1.0 323,561 + GFF2897 0.74 +0.2 323,562 - GFF2897 0.74 -0.1 323,562 - GFF2897 0.74 -0.9 323,562 - GFF2897 0.74 +0.5 323,562 - GFF2897 0.74 +0.2 323,562 - GFF2897 0.74 -0.0 323,562 - GFF2897 0.74 +0.0 323,562 - GFF2897 0.74 -1.4 323,562 - GFF2897 0.74 -0.6 323,562 - GFF2897 0.74 +1.0 323,562 - GFF2897 0.74 -0.1 323,562 - GFF2897 0.74 -0.4 323,716 - -0.3 323,716 - +0.4 323,841 + -0.3 323,841 + -1.0 323,841 + -0.1 323,841 + +0.7 323,841 + -0.4 323,842 - -1.1 323,842 - -0.5 323,842 - +0.2 323,842 - +0.3 323,842 - +0.3 323,925 + GFF2898 0.12 +0.6 323,925 + GFF2898 0.12 -0.4 323,925 + GFF2898 0.12 -1.5 323,925 + GFF2898 0.12 -0.4 323,926 - GFF2898 0.12 -0.9 323,926 - GFF2898 0.12 -0.5 323,930 - GFF2898 0.12 +0.2 323,943 + GFF2898 0.13 -0.8 323,943 + GFF2898 0.13 -0.0 323,943 + GFF2898 0.13 +1.1 323,944 - GFF2898 0.13 -0.9 323,944 - GFF2898 0.13 -0.5 323,944 - GFF2898 0.13 -0.2 323,944 - GFF2898 0.13 -1.2
Or see this region's nucleotide sequence