Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2893 and GFF2894 are separated by 41 nucleotides GFF2894 and GFF2895 overlap by 4 nucleotides GFF2895 and GFF2896 are separated by 146 nucleotides
GFF2893 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 318,841 to 319,827
GFF2893
GFF2894 - Transcriptional regulator, GntR family, at 319,869 to 320,651
GFF2894
GFF2895 - Vanillate O-demethylase oxygenase subunit (EC 1.14.13.82), at 320,648 to 321,766
GFF2895
GFF2896 - Methionine synthase II (cobalamin-independent), at 321,913 to 323,034
GFF2896
Position (kb) 320
321
322 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 319.652 kb on + strand, within GFF2893 at 319.652 kb on + strand, within GFF2893 at 319.653 kb on - strand, within GFF2893 at 319.718 kb on + strand, within GFF2893 at 320.216 kb on - strand, within GFF2894 at 320.216 kb on - strand, within GFF2894 at 320.410 kb on + strand, within GFF2894 at 320.411 kb on - strand, within GFF2894 at 320.781 kb on + strand, within GFF2895 at 320.781 kb on + strand, within GFF2895 at 320.781 kb on + strand, within GFF2895 at 320.782 kb on - strand, within GFF2895 at 320.782 kb on - strand, within GFF2895 at 320.782 kb on - strand, within GFF2895 at 320.907 kb on + strand, within GFF2895 at 320.907 kb on + strand, within GFF2895 at 320.907 kb on + strand, within GFF2895 at 320.907 kb on + strand, within GFF2895 at 320.907 kb on + strand, within GFF2895 at 320.908 kb on - strand, within GFF2895 at 320.908 kb on - strand, within GFF2895 at 320.908 kb on - strand, within GFF2895 at 320.908 kb on - strand, within GFF2895 at 320.931 kb on + strand, within GFF2895 at 320.931 kb on + strand, within GFF2895 at 320.932 kb on - strand, within GFF2895 at 320.932 kb on - strand, within GFF2895 at 320.932 kb on - strand, within GFF2895 at 320.932 kb on - strand, within GFF2895 at 320.976 kb on + strand, within GFF2895 at 321.531 kb on + strand, within GFF2895 at 321.532 kb on - strand, within GFF2895 at 321.532 kb on - strand, within GFF2895 at 321.532 kb on - strand, within GFF2895 at 321.652 kb on - strand, within GFF2895 at 321.652 kb on - strand, within GFF2895 at 321.726 kb on + strand at 321.726 kb on + strand at 321.726 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.818 kb on + strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.819 kb on - strand at 321.838 kb on - strand at 321.843 kb on + strand at 321.871 kb on + strand at 321.872 kb on - strand at 321.976 kb on + strand at 321.976 kb on + strand at 321.976 kb on + strand at 321.976 kb on + strand at 321.976 kb on + strand at 321.977 kb on - strand at 321.977 kb on - strand at 321.977 kb on - strand at 321.977 kb on - strand at 321.977 kb on - strand at 322.126 kb on + strand, within GFF2896 at 322.126 kb on + strand, within GFF2896 at 322.126 kb on + strand, within GFF2896 at 322.126 kb on + strand, within GFF2896 at 322.126 kb on + strand, within GFF2896 at 322.127 kb on - strand, within GFF2896 at 322.127 kb on - strand, within GFF2896 at 322.127 kb on - strand, within GFF2896 at 322.285 kb on + strand, within GFF2896 at 322.285 kb on + strand, within GFF2896 at 322.286 kb on - strand, within GFF2896 at 322.415 kb on - strand, within GFF2896 at 322.435 kb on + strand, within GFF2896 at 322.435 kb on + strand, within GFF2896 at 322.435 kb on + strand, within GFF2896 at 322.435 kb on + strand, within GFF2896 at 322.435 kb on + strand, within GFF2896 at 322.436 kb on - strand, within GFF2896 at 322.477 kb on + strand, within GFF2896 at 322.477 kb on + strand, within GFF2896 at 322.477 kb on + strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.478 kb on - strand, within GFF2896 at 322.507 kb on + strand, within GFF2896 at 322.507 kb on + strand, within GFF2896 at 322.507 kb on + strand, within GFF2896 at 322.507 kb on + strand, within GFF2896 at 322.508 kb on - strand, within GFF2896 at 322.508 kb on - strand, within GFF2896 at 322.508 kb on - strand, within GFF2896 at 322.508 kb on - strand, within GFF2896 at 322.508 kb on - strand, within GFF2896 at 322.508 kb on - strand, within GFF2896 at 322.687 kb on + strand, within GFF2896 at 322.687 kb on + strand, within GFF2896 at 322.687 kb on + strand, within GFF2896 at 322.687 kb on + strand, within GFF2896 at 322.688 kb on - strand, within GFF2896 at 322.688 kb on - strand, within GFF2896 at 322.688 kb on - strand, within GFF2896 at 322.688 kb on - strand, within GFF2896 at 322.688 kb on - strand, within GFF2896 at 322.688 kb on - strand, within GFF2896 at 322.735 kb on + strand, within GFF2896 at 322.736 kb on - strand, within GFF2896 at 322.736 kb on - strand, within GFF2896 at 322.736 kb on - strand, within GFF2896 at 322.747 kb on + strand, within GFF2896 at 322.748 kb on - strand, within GFF2896 at 322.748 kb on - strand, within GFF2896
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 319,652 + GFF2893 0.82 +0.4 319,652 + GFF2893 0.82 +0.0 319,653 - GFF2893 0.82 -0.2 319,718 + GFF2893 0.89 -0.7 320,216 - GFF2894 0.44 -0.4 320,216 - GFF2894 0.44 -0.1 320,410 + GFF2894 0.69 -3.4 320,411 - GFF2894 0.69 +0.4 320,781 + GFF2895 0.12 +0.1 320,781 + GFF2895 0.12 -0.2 320,781 + GFF2895 0.12 +0.5 320,782 - GFF2895 0.12 -0.0 320,782 - GFF2895 0.12 -0.1 320,782 - GFF2895 0.12 -2.0 320,907 + GFF2895 0.23 -0.1 320,907 + GFF2895 0.23 +0.5 320,907 + GFF2895 0.23 -0.8 320,907 + GFF2895 0.23 +1.1 320,907 + GFF2895 0.23 +0.3 320,908 - GFF2895 0.23 +0.9 320,908 - GFF2895 0.23 +0.2 320,908 - GFF2895 0.23 +0.4 320,908 - GFF2895 0.23 +0.2 320,931 + GFF2895 0.25 -0.5 320,931 + GFF2895 0.25 -1.0 320,932 - GFF2895 0.25 -0.6 320,932 - GFF2895 0.25 -0.8 320,932 - GFF2895 0.25 -1.0 320,932 - GFF2895 0.25 -1.2 320,976 + GFF2895 0.29 -0.2 321,531 + GFF2895 0.79 -0.9 321,532 - GFF2895 0.79 -0.4 321,532 - GFF2895 0.79 -1.0 321,532 - GFF2895 0.79 -1.3 321,652 - GFF2895 0.90 +0.1 321,652 - GFF2895 0.90 -0.6 321,726 + -0.0 321,726 + -0.6 321,726 + -0.4 321,818 + -1.0 321,818 + -0.6 321,818 + -1.2 321,818 + -0.3 321,818 + -1.1 321,818 + +1.9 321,818 + -1.3 321,818 + +1.8 321,818 + +0.3 321,818 + -0.3 321,818 + -0.5 321,818 + -0.9 321,818 + -0.4 321,818 + -1.3 321,818 + +0.4 321,818 + -0.3 321,818 + -2.1 321,818 + -0.2 321,818 + -1.0 321,818 + -1.2 321,818 + -0.4 321,818 + -0.7 321,818 + -1.4 321,818 + +0.0 321,818 + +1.3 321,818 + +0.1 321,818 + +1.2 321,818 + -0.6 321,818 + -0.7 321,818 + +0.0 321,818 + +0.3 321,818 + +0.6 321,818 + -1.2 321,818 + -0.7 321,818 + +0.3 321,818 + -1.0 321,818 + +0.4 321,818 + +1.2 321,818 + -1.6 321,818 + -0.2 321,818 + +0.6 321,818 + +0.6 321,818 + +1.3 321,818 + -0.7 321,818 + -0.9 321,818 + +0.1 321,818 + -1.0 321,818 + -0.8 321,818 + -0.0 321,818 + +0.8 321,818 + -2.0 321,818 + -0.6 321,818 + +0.8 321,818 + -0.3 321,819 - +0.7 321,819 - -0.4 321,819 - -0.4 321,819 - +1.1 321,819 - -0.3 321,819 - +0.3 321,819 - -0.8 321,819 - +0.2 321,819 - +0.2 321,819 - +0.4 321,819 - -3.1 321,819 - -0.6 321,819 - -0.7 321,819 - -1.0 321,819 - +0.2 321,819 - -0.9 321,819 - +1.5 321,819 - +0.6 321,819 - +0.3 321,819 - +0.1 321,819 - -0.2 321,819 - -0.6 321,819 - -0.9 321,819 - +0.5 321,819 - -1.3 321,819 - +0.1 321,819 - -0.5 321,819 - +0.7 321,819 - -0.1 321,819 - +0.0 321,819 - -0.7 321,819 - -0.2 321,819 - -0.0 321,819 - +1.6 321,819 - -1.4 321,819 - -1.1 321,819 - +1.4 321,819 - +0.7 321,819 - +0.3 321,819 - -0.8 321,819 - +0.5 321,819 - -2.3 321,819 - +0.2 321,819 - +0.6 321,819 - -0.2 321,838 - -1.3 321,843 + -0.3 321,871 + -0.6 321,872 - -0.1 321,976 + -0.8 321,976 + +0.1 321,976 + +0.8 321,976 + +0.1 321,976 + -1.3 321,977 - -0.3 321,977 - +0.6 321,977 - -0.4 321,977 - +0.3 321,977 - -0.1 322,126 + GFF2896 0.19 +0.4 322,126 + GFF2896 0.19 +0.8 322,126 + GFF2896 0.19 +0.7 322,126 + GFF2896 0.19 -0.1 322,126 + GFF2896 0.19 -1.7 322,127 - GFF2896 0.19 -1.5 322,127 - GFF2896 0.19 -1.1 322,127 - GFF2896 0.19 +0.1 322,285 + GFF2896 0.33 -1.4 322,285 + GFF2896 0.33 -0.7 322,286 - GFF2896 0.33 -0.8 322,415 - GFF2896 0.45 +0.2 322,435 + GFF2896 0.47 -1.7 322,435 + GFF2896 0.47 -0.0 322,435 + GFF2896 0.47 -0.2 322,435 + GFF2896 0.47 +0.0 322,435 + GFF2896 0.47 +0.0 322,436 - GFF2896 0.47 -0.1 322,477 + GFF2896 0.50 -0.9 322,477 + GFF2896 0.50 -0.1 322,477 + GFF2896 0.50 +0.1 322,478 - GFF2896 0.50 -0.1 322,478 - GFF2896 0.50 +0.0 322,478 - GFF2896 0.50 -0.6 322,478 - GFF2896 0.50 -0.4 322,478 - GFF2896 0.50 -0.2 322,478 - GFF2896 0.50 -1.2 322,478 - GFF2896 0.50 -1.0 322,507 + GFF2896 0.53 +1.2 322,507 + GFF2896 0.53 -1.2 322,507 + GFF2896 0.53 -1.1 322,507 + GFF2896 0.53 -0.6 322,508 - GFF2896 0.53 -1.4 322,508 - GFF2896 0.53 -1.6 322,508 - GFF2896 0.53 -0.1 322,508 - GFF2896 0.53 -0.6 322,508 - GFF2896 0.53 +0.1 322,508 - GFF2896 0.53 -1.2 322,687 + GFF2896 0.69 +0.2 322,687 + GFF2896 0.69 -0.6 322,687 + GFF2896 0.69 -0.2 322,687 + GFF2896 0.69 -0.6 322,688 - GFF2896 0.69 -1.0 322,688 - GFF2896 0.69 -0.9 322,688 - GFF2896 0.69 -0.1 322,688 - GFF2896 0.69 -1.7 322,688 - GFF2896 0.69 +1.0 322,688 - GFF2896 0.69 -0.2 322,735 + GFF2896 0.73 +0.0 322,736 - GFF2896 0.73 -0.6 322,736 - GFF2896 0.73 -1.0 322,736 - GFF2896 0.73 -0.5 322,747 + GFF2896 0.74 +0.2 322,748 - GFF2896 0.74 -0.3 322,748 - GFF2896 0.74 -0.1
Or see this region's nucleotide sequence