Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2269 and GFF2270 are separated by 5 nucleotides GFF2270 and GFF2271 are separated by 14 nucleotides GFF2271 and GFF2272 are separated by 43 nucleotides GFF2272 and GFF2273 are separated by 132 nucleotides
GFF2269 - Uncharacterized MFS-type transporter, at 323,347 to 324,813
GFF2269
GFF2270 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 324,819 to 325,238
GFF2270
GFF2271 - FIG00454722: hypothetical protein, at 325,253 to 325,780
GFF2271
GFF2272 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 325,824 to 326,267
GFF2272
GFF2273 - Transcriptional regulator, AcrR family, at 326,400 to 327,053
GFF2273
Position (kb) 325
326 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 324.272 kb on + strand, within GFF2269 at 324.273 kb on - strand, within GFF2269 at 324.273 kb on - strand, within GFF2269 at 324.273 kb on - strand, within GFF2269 at 324.593 kb on + strand, within GFF2269 at 324.594 kb on - strand, within GFF2269 at 324.594 kb on - strand, within GFF2269 at 324.647 kb on + strand, within GFF2269 at 324.647 kb on + strand, within GFF2269 at 324.648 kb on - strand, within GFF2269 at 324.648 kb on - strand, within GFF2269 at 324.648 kb on - strand, within GFF2269 at 324.648 kb on - strand, within GFF2269 at 324.820 kb on + strand at 325.015 kb on + strand, within GFF2270 at 325.016 kb on - strand, within GFF2270 at 325.016 kb on - strand, within GFF2270 at 325.016 kb on - strand, within GFF2270 at 325.022 kb on + strand, within GFF2270 at 325.178 kb on - strand, within GFF2270 at 325.220 kb on - strand at 325.390 kb on + strand, within GFF2271 at 325.390 kb on + strand, within GFF2271 at 325.390 kb on + strand, within GFF2271 at 325.391 kb on - strand, within GFF2271 at 325.542 kb on + strand, within GFF2271 at 325.542 kb on + strand, within GFF2271 at 325.542 kb on + strand, within GFF2271 at 325.542 kb on + strand, within GFF2271 at 325.542 kb on + strand, within GFF2271 at 325.542 kb on + strand, within GFF2271 at 325.542 kb on + strand, within GFF2271 at 325.542 kb on + strand, within GFF2271 at 325.542 kb on + strand, within GFF2271 at 325.542 kb on + strand, within GFF2271 at 325.543 kb on - strand, within GFF2271 at 325.608 kb on + strand, within GFF2271 at 325.608 kb on + strand, within GFF2271 at 325.608 kb on + strand, within GFF2271 at 325.609 kb on - strand, within GFF2271 at 326.167 kb on + strand, within GFF2272 at 326.209 kb on + strand, within GFF2272 at 326.209 kb on + strand, within GFF2272 at 326.209 kb on + strand, within GFF2272 at 326.209 kb on + strand, within GFF2272 at 326.209 kb on + strand, within GFF2272 at 326.209 kb on + strand, within GFF2272 at 326.210 kb on - strand, within GFF2272 at 326.210 kb on - strand, within GFF2272 at 326.210 kb on - strand, within GFF2272 at 326.251 kb on + strand at 326.251 kb on + strand at 326.251 kb on + strand at 326.251 kb on + strand at 326.252 kb on - strand at 326.252 kb on - strand at 326.252 kb on - strand at 326.252 kb on - strand at 326.363 kb on + strand at 326.364 kb on - strand at 326.574 kb on + strand, within GFF2273 at 326.574 kb on + strand, within GFF2273 at 326.574 kb on + strand, within GFF2273 at 326.575 kb on - strand, within GFF2273 at 326.575 kb on - strand, within GFF2273 at 326.733 kb on + strand, within GFF2273 at 326.733 kb on + strand, within GFF2273 at 326.734 kb on - strand, within GFF2273
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 324,272 + GFF2269 0.63 -0.7 324,273 - GFF2269 0.63 +1.0 324,273 - GFF2269 0.63 +0.1 324,273 - GFF2269 0.63 -0.5 324,593 + GFF2269 0.85 -2.1 324,594 - GFF2269 0.85 -0.5 324,594 - GFF2269 0.85 +0.2 324,647 + GFF2269 0.89 -0.6 324,647 + GFF2269 0.89 -0.4 324,648 - GFF2269 0.89 +0.4 324,648 - GFF2269 0.89 +0.6 324,648 - GFF2269 0.89 +0.2 324,648 - GFF2269 0.89 +0.4 324,820 + +0.6 325,015 + GFF2270 0.47 +0.3 325,016 - GFF2270 0.47 -0.8 325,016 - GFF2270 0.47 -1.3 325,016 - GFF2270 0.47 -1.4 325,022 + GFF2270 0.48 +0.3 325,178 - GFF2270 0.85 -0.7 325,220 - -0.1 325,390 + GFF2271 0.26 +1.3 325,390 + GFF2271 0.26 +0.3 325,390 + GFF2271 0.26 -2.8 325,391 - GFF2271 0.26 -1.8 325,542 + GFF2271 0.55 +0.8 325,542 + GFF2271 0.55 +0.2 325,542 + GFF2271 0.55 -0.1 325,542 + GFF2271 0.55 +0.2 325,542 + GFF2271 0.55 -0.2 325,542 + GFF2271 0.55 -0.4 325,542 + GFF2271 0.55 +0.2 325,542 + GFF2271 0.55 +0.9 325,542 + GFF2271 0.55 +0.6 325,542 + GFF2271 0.55 -0.0 325,543 - GFF2271 0.55 -1.4 325,608 + GFF2271 0.67 -0.6 325,608 + GFF2271 0.67 -0.7 325,608 + GFF2271 0.67 +0.0 325,609 - GFF2271 0.67 +0.9 326,167 + GFF2272 0.77 -1.1 326,209 + GFF2272 0.87 +0.3 326,209 + GFF2272 0.87 -0.4 326,209 + GFF2272 0.87 -0.1 326,209 + GFF2272 0.87 +0.1 326,209 + GFF2272 0.87 +0.9 326,209 + GFF2272 0.87 -0.5 326,210 - GFF2272 0.87 -1.2 326,210 - GFF2272 0.87 +0.2 326,210 - GFF2272 0.87 -0.2 326,251 + +1.8 326,251 + +1.2 326,251 + -0.2 326,251 + -1.4 326,252 - -0.3 326,252 - -0.4 326,252 - +0.0 326,252 - -1.3 326,363 + +3.8 326,364 - -0.7 326,574 + GFF2273 0.27 -0.0 326,574 + GFF2273 0.27 -1.9 326,574 + GFF2273 0.27 +1.0 326,575 - GFF2273 0.27 -1.4 326,575 - GFF2273 0.27 -0.8 326,733 + GFF2273 0.51 -1.4 326,733 + GFF2273 0.51 -0.4 326,734 - GFF2273 0.51 -1.9
Or see this region's nucleotide sequence