Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2235 and GFF2236 overlap by 4 nucleotides GFF2236 and GFF2237 are separated by 70 nucleotides GFF2237 and GFF2238 are separated by 122 nucleotides GFF2238 and GFF2239 are separated by 274 nucleotides
GFF2235 - hypothetical protein, at 296,103 to 296,834
GFF2235
GFF2236 - hypothetical protein, at 296,831 to 297,247
GFF2236
GFF2237 - Intracellular protease, at 297,318 to 297,896
GFF2237
GFF2238 - hypothetical protein, at 298,019 to 298,480
GFF2238
GFF2239 - no description, at 298,755 to 299,171
GFF2239
Position (kb) 297
298 Strain fitness (log2 ratio) -1
0
1 at 296.543 kb on + strand, within GFF2235 at 296.772 kb on + strand at 296.773 kb on - strand at 296.773 kb on - strand at 297.075 kb on + strand, within GFF2236 at 297.210 kb on + strand at 297.210 kb on + strand at 297.295 kb on - strand at 297.442 kb on + strand, within GFF2237 at 297.442 kb on + strand, within GFF2237 at 297.442 kb on + strand, within GFF2237 at 297.443 kb on - strand, within GFF2237 at 297.443 kb on - strand, within GFF2237 at 297.620 kb on - strand, within GFF2237 at 297.659 kb on - strand, within GFF2237 at 297.863 kb on - strand at 297.955 kb on + strand at 297.956 kb on - strand at 298.072 kb on - strand, within GFF2238 at 298.204 kb on - strand, within GFF2238 at 298.243 kb on - strand, within GFF2238 at 298.243 kb on - strand, within GFF2238 at 298.580 kb on - strand at 298.580 kb on - strand at 298.580 kb on - strand at 298.633 kb on + strand at 298.633 kb on + strand at 298.634 kb on - strand at 298.634 kb on - strand at 298.679 kb on - strand at 298.679 kb on - strand at 298.679 kb on - strand at 298.739 kb on + strand at 298.739 kb on + strand at 298.739 kb on + strand at 298.739 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 296,543 + GFF2235 0.60 -0.0 296,772 + +0.1 296,773 - +0.4 296,773 - -0.9 297,075 + GFF2236 0.59 +0.6 297,210 + -0.4 297,210 + -0.2 297,295 - +0.1 297,442 + GFF2237 0.21 +0.2 297,442 + GFF2237 0.21 -1.0 297,442 + GFF2237 0.21 +0.1 297,443 - GFF2237 0.22 -0.3 297,443 - GFF2237 0.22 +0.5 297,620 - GFF2237 0.52 +0.5 297,659 - GFF2237 0.59 -0.3 297,863 - +0.4 297,955 + -0.3 297,956 - -0.6 298,072 - GFF2238 0.11 +0.1 298,204 - GFF2238 0.40 -0.2 298,243 - GFF2238 0.48 -1.2 298,243 - GFF2238 0.48 +0.5 298,580 - +0.4 298,580 - -0.8 298,580 - -0.5 298,633 + -1.4 298,633 + +0.1 298,634 - -0.5 298,634 - -0.0 298,679 - +1.5 298,679 - +1.7 298,679 - -1.3 298,739 + -0.9 298,739 + +0.0 298,739 + +0.2 298,739 + -0.1
Or see this region's nucleotide sequence