Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2073 and GFF2074 overlap by 4 nucleotides GFF2074 and GFF2075 are separated by 4 nucleotides GFF2075 and GFF2076 are separated by 24 nucleotides
GFF2073 - Holliday junction ATP-dependent DNA helicase RuvA (EC 3.6.4.12), at 119,705 to 120,277
GFF2073
GFF2074 - hypothetical protein, at 120,274 to 121,161
GFF2074
GFF2075 - BUG/TctC family periplasmic protein, at 121,166 to 122,161
GFF2075
GFF2076 - Alkyl sulfatase and related hydrolases, MBL-fold metallo-hydrolase superfamily, at 122,186 to 123,457
GFF2076
Position (kb)
121
122
123 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 120.388 kb on + strand, within GFF2074 at 120.409 kb on + strand, within GFF2074 at 120.410 kb on - strand, within GFF2074 at 120.410 kb on - strand, within GFF2074 at 121.054 kb on + strand, within GFF2074 at 121.055 kb on - strand, within GFF2074 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.587 kb on + strand, within GFF2075 at 121.587 kb on + strand, within GFF2075 at 121.588 kb on - strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.704 kb on + strand, within GFF2075 at 121.827 kb on + strand, within GFF2075 at 121.828 kb on - strand, within GFF2075 at 121.828 kb on - strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 122.061 kb on + strand, within GFF2075 at 122.061 kb on + strand, within GFF2075 at 122.062 kb on - strand at 122.062 kb on - strand at 122.244 kb on + strand at 122.245 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.532 kb on + strand, within GFF2076 at 122.532 kb on + strand, within GFF2076 at 122.532 kb on + strand, within GFF2076 at 122.532 kb on + strand, within GFF2076 at 122.533 kb on - strand, within GFF2076 at 122.533 kb on - strand, within GFF2076 at 122.533 kb on - strand, within GFF2076 at 122.550 kb on + strand, within GFF2076 at 122.550 kb on + strand, within GFF2076 at 122.550 kb on + strand, within GFF2076 at 122.565 kb on + strand, within GFF2076 at 122.566 kb on - strand, within GFF2076 at 122.971 kb on + strand, within GFF2076 at 122.972 kb on + strand, within GFF2076 at 122.973 kb on - strand, within GFF2076 at 122.973 kb on - strand, within GFF2076 at 122.973 kb on - strand, within GFF2076
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 120,388 + GFF2074 0.13 -0.6 120,409 + GFF2074 0.15 +0.8 120,410 - GFF2074 0.15 -0.7 120,410 - GFF2074 0.15 -0.6 121,054 + GFF2074 0.88 +1.8 121,055 - GFF2074 0.88 +0.1 121,395 + GFF2075 0.23 -2.1 121,395 + GFF2075 0.23 +1.6 121,395 + GFF2075 0.23 -0.8 121,395 + GFF2075 0.23 -0.1 121,395 + GFF2075 0.23 -0.6 121,396 - GFF2075 0.23 +1.3 121,396 - GFF2075 0.23 -1.4 121,396 - GFF2075 0.23 -1.3 121,396 - GFF2075 0.23 -2.0 121,587 + GFF2075 0.42 +0.1 121,587 + GFF2075 0.42 -1.0 121,588 - GFF2075 0.42 -0.4 121,674 + GFF2075 0.51 +0.1 121,674 + GFF2075 0.51 -0.2 121,674 + GFF2075 0.51 -0.2 121,674 + GFF2075 0.51 -2.5 121,674 + GFF2075 0.51 -0.8 121,675 - GFF2075 0.51 -0.3 121,675 - GFF2075 0.51 -0.0 121,675 - GFF2075 0.51 +0.3 121,675 - GFF2075 0.51 -1.4 121,675 - GFF2075 0.51 +0.6 121,675 - GFF2075 0.51 +0.2 121,704 + GFF2075 0.54 -1.5 121,827 + GFF2075 0.66 -0.2 121,828 - GFF2075 0.66 -0.9 121,828 - GFF2075 0.66 -1.4 121,860 + GFF2075 0.70 -1.0 121,860 + GFF2075 0.70 -0.5 121,860 + GFF2075 0.70 +0.0 121,860 + GFF2075 0.70 -2.2 121,860 + GFF2075 0.70 -0.3 122,061 + GFF2075 0.90 +0.0 122,061 + GFF2075 0.90 -0.4 122,062 - -1.7 122,062 - -0.8 122,244 + -0.6 122,245 - -0.2 122,263 - +0.4 122,263 - -1.0 122,263 - -0.5 122,263 - -0.4 122,263 - -0.3 122,263 - -0.4 122,532 + GFF2076 0.27 -3.0 122,532 + GFF2076 0.27 -0.3 122,532 + GFF2076 0.27 +0.2 122,532 + GFF2076 0.27 -0.3 122,533 - GFF2076 0.27 -0.2 122,533 - GFF2076 0.27 -0.6 122,533 - GFF2076 0.27 -0.4 122,550 + GFF2076 0.29 +0.9 122,550 + GFF2076 0.29 -0.4 122,550 + GFF2076 0.29 +1.0 122,565 + GFF2076 0.30 -0.7 122,566 - GFF2076 0.30 +1.8 122,971 + GFF2076 0.62 +0.3 122,972 + GFF2076 0.62 +1.8 122,973 - GFF2076 0.62 -0.3 122,973 - GFF2076 0.62 +1.3 122,973 - GFF2076 0.62 -0.2
Or see this region's nucleotide sequence