Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1639 and GFF1640 overlap by 4 nucleotides GFF1640 and GFF1641 overlap by 1 nucleotides
GFF1639 - Polyketide synthase modules and related proteins, at 268,110 to 271,196
GFF1639
GFF1640 - Polyketide synthase modules and related proteins, at 271,193 to 278,275
GFF1640
GFF1641 - Polyketide synthase modules and related proteins, at 278,275 to 283,587
GFF1641
Position (kb)
271
272
273
274
275
276
277
278
279 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 270.635 kb on - strand, within GFF1639 at 270.700 kb on + strand, within GFF1639 at 270.743 kb on - strand, within GFF1639 at 271.309 kb on - strand at 271.449 kb on + strand at 271.695 kb on + strand at 271.696 kb on - strand at 271.743 kb on + strand at 271.743 kb on + strand at 271.744 kb on - strand at 271.932 kb on + strand, within GFF1640 at 271.933 kb on - strand, within GFF1640 at 271.948 kb on - strand, within GFF1640 at 271.948 kb on - strand, within GFF1640 at 271.948 kb on - strand, within GFF1640 at 271.995 kb on + strand, within GFF1640 at 272.103 kb on + strand, within GFF1640 at 272.437 kb on - strand, within GFF1640 at 272.451 kb on + strand, within GFF1640 at 272.451 kb on + strand, within GFF1640 at 272.914 kb on + strand, within GFF1640 at 273.058 kb on - strand, within GFF1640 at 273.115 kb on - strand, within GFF1640 at 273.126 kb on + strand, within GFF1640 at 273.126 kb on + strand, within GFF1640 at 273.135 kb on + strand, within GFF1640 at 273.135 kb on + strand, within GFF1640 at 273.135 kb on + strand, within GFF1640 at 273.136 kb on - strand, within GFF1640 at 273.190 kb on - strand, within GFF1640 at 273.807 kb on + strand, within GFF1640 at 273.807 kb on + strand, within GFF1640 at 273.807 kb on + strand, within GFF1640 at 274.299 kb on + strand, within GFF1640 at 274.299 kb on + strand, within GFF1640 at 274.788 kb on + strand, within GFF1640 at 274.789 kb on - strand, within GFF1640 at 274.789 kb on - strand, within GFF1640 at 274.845 kb on + strand, within GFF1640 at 274.845 kb on + strand, within GFF1640 at 274.845 kb on + strand, within GFF1640 at 274.846 kb on - strand, within GFF1640 at 275.499 kb on - strand, within GFF1640 at 275.499 kb on - strand, within GFF1640 at 275.529 kb on + strand, within GFF1640 at 275.529 kb on + strand, within GFF1640 at 275.529 kb on + strand, within GFF1640 at 275.530 kb on - strand, within GFF1640 at 275.530 kb on - strand, within GFF1640 at 275.530 kb on - strand, within GFF1640 at 275.530 kb on - strand, within GFF1640 at 276.342 kb on + strand, within GFF1640 at 276.342 kb on + strand, within GFF1640 at 276.414 kb on + strand, within GFF1640 at 276.415 kb on - strand, within GFF1640 at 277.050 kb on + strand, within GFF1640 at 277.050 kb on + strand, within GFF1640 at 277.051 kb on - strand, within GFF1640 at 277.051 kb on - strand, within GFF1640 at 277.593 kb on + strand at 277.755 kb on + strand at 277.756 kb on - strand at 277.756 kb on - strand at 277.869 kb on + strand at 278.074 kb on - strand at 278.074 kb on - strand at 278.637 kb on - strand at 278.744 kb on + strand at 278.744 kb on + strand at 278.744 kb on + strand at 278.744 kb on + strand at 278.745 kb on - strand at 278.745 kb on - strand at 278.745 kb on - strand at 278.819 kb on + strand, within GFF1641 at 278.820 kb on - strand, within GFF1641 at 278.820 kb on - strand, within GFF1641 at 278.820 kb on - strand, within GFF1641 at 278.967 kb on - strand, within GFF1641 at 279.086 kb on + strand, within GFF1641 at 279.116 kb on + strand, within GFF1641 at 279.116 kb on + strand, within GFF1641 at 279.117 kb on - strand, within GFF1641 at 279.209 kb on + strand, within GFF1641 at 279.209 kb on + strand, within GFF1641 at 279.210 kb on - strand, within GFF1641 at 279.210 kb on - strand, within GFF1641
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 270,635 - GFF1639 0.82 +0.3 270,700 + GFF1639 0.84 +0.2 270,743 - GFF1639 0.85 +0.3 271,309 - -0.4 271,449 + -0.7 271,695 + +0.2 271,696 - -0.9 271,743 + -0.7 271,743 + -1.6 271,744 - -0.5 271,932 + GFF1640 0.10 -1.8 271,933 - GFF1640 0.10 -0.2 271,948 - GFF1640 0.11 -1.0 271,948 - GFF1640 0.11 -0.9 271,948 - GFF1640 0.11 +0.2 271,995 + GFF1640 0.11 -0.5 272,103 + GFF1640 0.13 -0.4 272,437 - GFF1640 0.18 -1.6 272,451 + GFF1640 0.18 +1.3 272,451 + GFF1640 0.18 -0.4 272,914 + GFF1640 0.24 -0.7 273,058 - GFF1640 0.26 -1.0 273,115 - GFF1640 0.27 -0.6 273,126 + GFF1640 0.27 +1.2 273,126 + GFF1640 0.27 +0.8 273,135 + GFF1640 0.27 -0.7 273,135 + GFF1640 0.27 -0.3 273,135 + GFF1640 0.27 +0.8 273,136 - GFF1640 0.27 -1.2 273,190 - GFF1640 0.28 +0.6 273,807 + GFF1640 0.37 -0.5 273,807 + GFF1640 0.37 +0.2 273,807 + GFF1640 0.37 -0.8 274,299 + GFF1640 0.44 +0.9 274,299 + GFF1640 0.44 +0.0 274,788 + GFF1640 0.51 -0.2 274,789 - GFF1640 0.51 -0.7 274,789 - GFF1640 0.51 +0.8 274,845 + GFF1640 0.52 -2.0 274,845 + GFF1640 0.52 +0.5 274,845 + GFF1640 0.52 -1.0 274,846 - GFF1640 0.52 -0.4 275,499 - GFF1640 0.61 +0.6 275,499 - GFF1640 0.61 -0.7 275,529 + GFF1640 0.61 +0.3 275,529 + GFF1640 0.61 -2.6 275,529 + GFF1640 0.61 -0.7 275,530 - GFF1640 0.61 -0.2 275,530 - GFF1640 0.61 -0.5 275,530 - GFF1640 0.61 -0.5 275,530 - GFF1640 0.61 +0.1 276,342 + GFF1640 0.73 -0.5 276,342 + GFF1640 0.73 +0.8 276,414 + GFF1640 0.74 +0.0 276,415 - GFF1640 0.74 -0.4 277,050 + GFF1640 0.83 -0.1 277,050 + GFF1640 0.83 +0.8 277,051 - GFF1640 0.83 -0.7 277,051 - GFF1640 0.83 +0.0 277,593 + -0.2 277,755 + -0.6 277,756 - -0.1 277,756 - -0.4 277,869 + -2.0 278,074 - +0.4 278,074 - -1.5 278,637 - +0.4 278,744 + -0.0 278,744 + +0.4 278,744 + -0.6 278,744 + -1.1 278,745 - +0.2 278,745 - +0.8 278,745 - +0.4 278,819 + GFF1641 0.10 -1.4 278,820 - GFF1641 0.10 -1.0 278,820 - GFF1641 0.10 -0.1 278,820 - GFF1641 0.10 -2.1 278,967 - GFF1641 0.13 -0.8 279,086 + GFF1641 0.15 -0.8 279,116 + GFF1641 0.16 +2.3 279,116 + GFF1641 0.16 -1.3 279,117 - GFF1641 0.16 +0.1 279,209 + GFF1641 0.18 -1.6 279,209 + GFF1641 0.18 -0.6 279,210 - GFF1641 0.18 +0.7 279,210 - GFF1641 0.18 -0.0
Or see this region's nucleotide sequence