Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1582 and GFF1583 are separated by 22 nucleotides GFF1583 and GFF1584 are separated by 6 nucleotides
GFF1582 - BUG/TctC family periplasmic protein, at 181,135 to 182,139
GFF1582
GFF1583 - Fumarate hydratase class I, beta region (EC 4.2.1.2); L(+)-tartrate dehydratase beta subunit (EC 4.2.1.32), at 182,162 to 182,779
GFF1583
GFF1584 - Fumarate hydratase class I, alpha region (EC 4.2.1.2); L(+)-tartrate dehydratase alpha subunit (EC 4.2.1.32), at 182,786 to 183,658
GFF1584
Position (kb) 182
183 Strain fitness (log2 ratio) -2
-1
0
1 at 181.553 kb on + strand, within GFF1582 at 181.554 kb on - strand, within GFF1582 at 181.796 kb on + strand, within GFF1582 at 181.797 kb on - strand, within GFF1582 at 181.847 kb on + strand, within GFF1582 at 182.331 kb on + strand, within GFF1583 at 182.409 kb on + strand, within GFF1583 at 182.409 kb on + strand, within GFF1583 at 182.409 kb on + strand, within GFF1583 at 182.409 kb on + strand, within GFF1583 at 182.410 kb on - strand, within GFF1583 at 182.548 kb on - strand, within GFF1583 at 182.787 kb on + strand at 182.788 kb on - strand at 182.820 kb on + strand at 182.820 kb on + strand at 182.821 kb on - strand at 182.876 kb on + strand, within GFF1584 at 183.246 kb on + strand, within GFF1584 at 183.247 kb on - strand, within GFF1584 at 183.301 kb on - strand, within GFF1584 at 183.301 kb on - strand, within GFF1584 at 183.442 kb on - strand, within GFF1584 at 183.442 kb on - strand, within GFF1584 at 183.442 kb on - strand, within GFF1584 at 183.612 kb on + strand at 183.612 kb on + strand at 183.663 kb on - strand at 183.663 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 181,553 + GFF1582 0.42 -0.4 181,554 - GFF1582 0.42 +0.3 181,796 + GFF1582 0.66 +0.5 181,797 - GFF1582 0.66 -1.0 181,847 + GFF1582 0.71 +1.0 182,331 + GFF1583 0.27 -0.2 182,409 + GFF1583 0.40 -0.4 182,409 + GFF1583 0.40 -2.2 182,409 + GFF1583 0.40 -0.3 182,409 + GFF1583 0.40 -0.7 182,410 - GFF1583 0.40 -0.4 182,548 - GFF1583 0.62 +0.4 182,787 + +0.2 182,788 - -0.9 182,820 + -0.1 182,820 + +0.0 182,821 - +1.4 182,876 + GFF1584 0.10 -0.7 183,246 + GFF1584 0.53 -0.9 183,247 - GFF1584 0.53 +1.4 183,301 - GFF1584 0.59 +0.0 183,301 - GFF1584 0.59 -0.3 183,442 - GFF1584 0.75 -0.9 183,442 - GFF1584 0.75 -0.3 183,442 - GFF1584 0.75 -0.4 183,612 + -0.8 183,612 + +0.5 183,663 - -0.4 183,663 - +0.8
Or see this region's nucleotide sequence