Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1489 and GFF1490 are separated by 3 nucleotides GFF1490 and GFF1491 are separated by 35 nucleotides GFF1491 and GFF1492 are separated by 1 nucleotides GFF1492 and GFF1493 are separated by 8 nucleotides
GFF1489 - Tetrapartite efflux system, outer membrane factor lipoprotein FusA-like, at 81,910 to 83,373
GFF1489
GFF1490 - Transcriptional regulator, LysR family, at 83,377 to 84,273
GFF1490
GFF1491 - Gluconolactonase, at 84,309 to 85,241
GFF1491
GFF1492 - putative 2-pyrone-4,6-dicarboxylic acid hydrolase, at 85,243 to 86,142
GFF1492
GFF1493 - BUG/TctC family periplasmic protein, at 86,151 to 87,116
GFF1493
Position (kb)
84
85
86 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 83.371 kb on + strand at 83.887 kb on + strand, within GFF1490 at 83.950 kb on + strand, within GFF1490 at 83.951 kb on - strand, within GFF1490 at 83.951 kb on - strand, within GFF1490 at 83.951 kb on - strand, within GFF1490 at 84.007 kb on + strand, within GFF1490 at 84.007 kb on + strand, within GFF1490 at 84.007 kb on + strand, within GFF1490 at 84.008 kb on - strand, within GFF1490 at 84.102 kb on - strand, within GFF1490 at 84.102 kb on - strand, within GFF1490 at 84.187 kb on + strand at 84.187 kb on + strand at 84.188 kb on - strand at 84.188 kb on - strand at 84.310 kb on + strand at 84.310 kb on + strand at 84.311 kb on - strand at 84.437 kb on + strand, within GFF1491 at 84.511 kb on + strand, within GFF1491 at 84.676 kb on + strand, within GFF1491 at 84.676 kb on + strand, within GFF1491 at 84.676 kb on + strand, within GFF1491 at 84.676 kb on + strand, within GFF1491 at 84.820 kb on + strand, within GFF1491 at 84.944 kb on - strand, within GFF1491 at 84.944 kb on - strand, within GFF1491 at 84.944 kb on - strand, within GFF1491 at 85.003 kb on + strand, within GFF1491 at 85.062 kb on + strand, within GFF1491 at 85.062 kb on + strand, within GFF1491 at 85.063 kb on - strand, within GFF1491 at 85.063 kb on - strand, within GFF1491 at 85.063 kb on - strand, within GFF1491 at 85.075 kb on + strand, within GFF1491 at 85.237 kb on + strand at 85.238 kb on - strand at 85.238 kb on - strand at 85.238 kb on - strand at 85.275 kb on - strand at 85.499 kb on + strand, within GFF1492 at 85.499 kb on + strand, within GFF1492 at 85.499 kb on + strand, within GFF1492 at 85.499 kb on + strand, within GFF1492 at 85.500 kb on - strand, within GFF1492 at 85.500 kb on - strand, within GFF1492 at 85.500 kb on - strand, within GFF1492 at 85.559 kb on + strand, within GFF1492 at 85.559 kb on + strand, within GFF1492 at 85.560 kb on - strand, within GFF1492 at 85.560 kb on - strand, within GFF1492 at 85.560 kb on - strand, within GFF1492 at 85.560 kb on - strand, within GFF1492 at 85.560 kb on - strand, within GFF1492 at 85.684 kb on + strand, within GFF1492 at 85.684 kb on + strand, within GFF1492 at 85.684 kb on + strand, within GFF1492 at 85.684 kb on + strand, within GFF1492 at 85.684 kb on + strand, within GFF1492 at 85.685 kb on - strand, within GFF1492 at 85.685 kb on - strand, within GFF1492 at 85.685 kb on - strand, within GFF1492 at 85.685 kb on - strand, within GFF1492 at 86.203 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 83,371 + -0.9 83,887 + GFF1490 0.57 -1.3 83,950 + GFF1490 0.64 -0.1 83,951 - GFF1490 0.64 +0.1 83,951 - GFF1490 0.64 -0.0 83,951 - GFF1490 0.64 -2.4 84,007 + GFF1490 0.70 -0.2 84,007 + GFF1490 0.70 +3.3 84,007 + GFF1490 0.70 +0.0 84,008 - GFF1490 0.70 +0.1 84,102 - GFF1490 0.81 +0.9 84,102 - GFF1490 0.81 -0.4 84,187 + -0.3 84,187 + -0.2 84,188 - -2.3 84,188 - +0.7 84,310 + +0.7 84,310 + -1.2 84,311 - -0.3 84,437 + GFF1491 0.14 -0.7 84,511 + GFF1491 0.22 +0.1 84,676 + GFF1491 0.39 -0.6 84,676 + GFF1491 0.39 +0.6 84,676 + GFF1491 0.39 +1.0 84,676 + GFF1491 0.39 -1.0 84,820 + GFF1491 0.55 -1.7 84,944 - GFF1491 0.68 +0.9 84,944 - GFF1491 0.68 -0.4 84,944 - GFF1491 0.68 +0.7 85,003 + GFF1491 0.74 +0.5 85,062 + GFF1491 0.81 -0.3 85,062 + GFF1491 0.81 -0.1 85,063 - GFF1491 0.81 +0.4 85,063 - GFF1491 0.81 +0.7 85,063 - GFF1491 0.81 +0.1 85,075 + GFF1491 0.82 +0.0 85,237 + +0.0 85,238 - +1.7 85,238 - -1.3 85,238 - -1.3 85,275 - +1.0 85,499 + GFF1492 0.28 +0.1 85,499 + GFF1492 0.28 -0.8 85,499 + GFF1492 0.28 +0.3 85,499 + GFF1492 0.28 -0.9 85,500 - GFF1492 0.29 -0.3 85,500 - GFF1492 0.29 -0.3 85,500 - GFF1492 0.29 +1.0 85,559 + GFF1492 0.35 -1.7 85,559 + GFF1492 0.35 -0.0 85,560 - GFF1492 0.35 -0.4 85,560 - GFF1492 0.35 -0.9 85,560 - GFF1492 0.35 -1.5 85,560 - GFF1492 0.35 -1.0 85,560 - GFF1492 0.35 -0.3 85,684 + GFF1492 0.49 +0.0 85,684 + GFF1492 0.49 +0.5 85,684 + GFF1492 0.49 -0.9 85,684 + GFF1492 0.49 -0.5 85,684 + GFF1492 0.49 +0.5 85,685 - GFF1492 0.49 -0.5 85,685 - GFF1492 0.49 -1.4 85,685 - GFF1492 0.49 +0.1 85,685 - GFF1492 0.49 -2.1 86,203 + -1.3
Or see this region's nucleotide sequence