Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1301 and GFF1302 are separated by 3 nucleotides GFF1302 and GFF1303 are separated by 150 nucleotides GFF1303 and GFF1304 are separated by 133 nucleotides
GFF1301 - Peptide deformylase (EC 3.5.1.88), at 632,522 to 633,061
GFF1301
GFF1302 - DoxX family protein, at 633,065 to 633,460
GFF1302
GFF1303 - Glutamate/aspartate ABC transporter, substrate-binding protein GltI (TC 3.A.1.3.4), at 633,611 to 634,648
GFF1303
GFF1304 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 634,782 to 635,789
GFF1304
Position (kb) 633
634
635 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 632.679 kb on + strand, within GFF1301 at 632.679 kb on + strand, within GFF1301 at 632.680 kb on - strand, within GFF1301 at 632.680 kb on - strand, within GFF1301 at 632.680 kb on - strand, within GFF1301 at 632.680 kb on - strand, within GFF1301 at 632.876 kb on + strand, within GFF1301 at 632.877 kb on - strand, within GFF1301 at 632.877 kb on - strand, within GFF1301 at 632.877 kb on - strand, within GFF1301 at 632.879 kb on - strand, within GFF1301 at 632.894 kb on + strand, within GFF1301 at 632.894 kb on + strand, within GFF1301 at 632.978 kb on + strand, within GFF1301 at 632.979 kb on - strand, within GFF1301 at 633.014 kb on + strand at 633.066 kb on + strand at 633.066 kb on + strand at 633.066 kb on + strand at 633.066 kb on + strand at 633.066 kb on + strand at 633.066 kb on + strand at 633.066 kb on + strand at 633.066 kb on + strand at 633.066 kb on + strand at 633.066 kb on + strand at 633.066 kb on + strand at 633.067 kb on - strand at 633.067 kb on - strand at 633.067 kb on - strand at 633.067 kb on - strand at 633.067 kb on - strand at 633.067 kb on - strand at 633.067 kb on - strand at 633.067 kb on - strand at 633.067 kb on - strand at 633.067 kb on - strand at 633.072 kb on + strand at 633.072 kb on + strand at 633.072 kb on + strand at 633.072 kb on + strand at 633.072 kb on + strand at 633.072 kb on + strand at 633.072 kb on + strand at 633.072 kb on + strand at 633.072 kb on + strand at 633.072 kb on + strand at 633.072 kb on + strand at 633.072 kb on + strand at 633.072 kb on + strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.073 kb on - strand at 633.258 kb on + strand, within GFF1302 at 633.258 kb on + strand, within GFF1302 at 633.258 kb on + strand, within GFF1302 at 633.258 kb on + strand, within GFF1302 at 633.259 kb on - strand, within GFF1302 at 633.259 kb on - strand, within GFF1302 at 633.259 kb on - strand, within GFF1302 at 633.259 kb on - strand, within GFF1302 at 633.303 kb on + strand, within GFF1302 at 633.303 kb on + strand, within GFF1302 at 633.303 kb on + strand, within GFF1302 at 633.303 kb on + strand, within GFF1302 at 633.303 kb on + strand, within GFF1302 at 633.303 kb on + strand, within GFF1302 at 633.303 kb on + strand, within GFF1302 at 633.304 kb on - strand, within GFF1302 at 633.304 kb on - strand, within GFF1302 at 633.304 kb on - strand, within GFF1302 at 633.304 kb on - strand, within GFF1302 at 633.304 kb on - strand, within GFF1302 at 633.304 kb on - strand, within GFF1302 at 633.312 kb on + strand, within GFF1302 at 633.313 kb on - strand, within GFF1302 at 633.313 kb on - strand, within GFF1302 at 633.313 kb on - strand, within GFF1302 at 633.466 kb on + strand at 633.467 kb on - strand at 633.467 kb on - strand at 633.467 kb on - strand at 633.467 kb on - strand at 633.496 kb on + strand at 633.496 kb on + strand at 633.496 kb on + strand at 633.497 kb on - strand at 633.497 kb on - strand at 633.501 kb on + strand at 633.501 kb on + strand at 633.502 kb on - strand at 633.502 kb on - strand at 633.502 kb on - strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.529 kb on + strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.530 kb on - strand at 633.533 kb on + strand at 633.533 kb on + strand at 633.533 kb on + strand at 633.533 kb on + strand at 633.533 kb on + strand at 633.533 kb on + strand at 633.533 kb on + strand at 633.533 kb on + strand at 633.533 kb on + strand at 633.533 kb on + strand at 633.534 kb on - strand at 633.534 kb on - strand at 633.534 kb on - strand at 633.534 kb on - strand at 633.534 kb on - strand at 633.534 kb on - strand at 633.534 kb on - strand at 633.534 kb on - strand at 633.534 kb on - strand at 633.575 kb on + strand at 633.575 kb on + strand at 633.575 kb on + strand at 633.575 kb on + strand at 633.575 kb on + strand at 633.575 kb on + strand at 633.576 kb on - strand at 633.576 kb on - strand at 633.576 kb on - strand at 633.576 kb on - strand at 633.576 kb on - strand at 633.576 kb on - strand at 633.576 kb on - strand at 633.576 kb on - strand at 633.576 kb on - strand at 633.576 kb on - strand at 633.591 kb on + strand at 633.591 kb on + strand at 633.592 kb on - strand at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.990 kb on - strand, within GFF1303 at 633.990 kb on - strand, within GFF1303 at 633.990 kb on - strand, within GFF1303 at 633.990 kb on - strand, within GFF1303 at 633.990 kb on - strand, within GFF1303 at 633.990 kb on - strand, within GFF1303 at 633.992 kb on + strand, within GFF1303 at 633.993 kb on - strand, within GFF1303 at 633.993 kb on - strand, within GFF1303 at 633.993 kb on - strand, within GFF1303 at 633.993 kb on - strand, within GFF1303 at 634.109 kb on + strand, within GFF1303 at 634.110 kb on - strand, within GFF1303 at 634.110 kb on - strand, within GFF1303 at 634.110 kb on - strand, within GFF1303 at 634.265 kb on + strand, within GFF1303 at 634.266 kb on - strand, within GFF1303 at 634.266 kb on - strand, within GFF1303 at 634.266 kb on - strand, within GFF1303 at 634.266 kb on - strand, within GFF1303 at 634.266 kb on - strand, within GFF1303 at 634.535 kb on + strand, within GFF1303 at 634.535 kb on + strand, within GFF1303 at 634.535 kb on + strand, within GFF1303 at 634.535 kb on + strand, within GFF1303 at 634.536 kb on - strand, within GFF1303 at 634.536 kb on - strand, within GFF1303 at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 635.145 kb on + strand, within GFF1304 at 635.145 kb on + strand, within GFF1304 at 635.145 kb on + strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.321 kb on + strand, within GFF1304 at 635.321 kb on + strand, within GFF1304 at 635.322 kb on - strand, within GFF1304 at 635.322 kb on - strand, within GFF1304 at 635.349 kb on - strand, within GFF1304 at 635.349 kb on - strand, within GFF1304 at 635.388 kb on + strand, within GFF1304 at 635.388 kb on + strand, within GFF1304 at 635.388 kb on + strand, within GFF1304 at 635.388 kb on + strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.416 kb on + strand, within GFF1304 at 635.419 kb on - strand, within GFF1304 at 635.419 kb on - strand, within GFF1304
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 632,679 + GFF1301 0.29 +0.3 632,679 + GFF1301 0.29 -0.3 632,680 - GFF1301 0.29 +0.2 632,680 - GFF1301 0.29 +0.4 632,680 - GFF1301 0.29 -1.3 632,680 - GFF1301 0.29 +0.5 632,876 + GFF1301 0.66 +2.4 632,877 - GFF1301 0.66 -0.8 632,877 - GFF1301 0.66 -0.5 632,877 - GFF1301 0.66 -1.1 632,879 - GFF1301 0.66 -1.7 632,894 + GFF1301 0.69 +0.2 632,894 + GFF1301 0.69 -0.4 632,978 + GFF1301 0.84 -0.3 632,979 - GFF1301 0.85 +1.4 633,014 + -0.1 633,066 + -0.9 633,066 + +0.1 633,066 + -0.3 633,066 + -0.6 633,066 + -0.1 633,066 + -1.9 633,066 + +0.4 633,066 + -1.2 633,066 + +1.2 633,066 + -0.7 633,066 + -0.9 633,067 - +0.8 633,067 - +0.2 633,067 - -0.3 633,067 - +0.1 633,067 - +0.4 633,067 - -0.5 633,067 - +0.1 633,067 - -0.5 633,067 - +0.7 633,067 - +0.0 633,072 + +0.6 633,072 + -0.9 633,072 + -0.8 633,072 + +0.1 633,072 + -1.3 633,072 + -0.1 633,072 + +0.6 633,072 + -0.1 633,072 + -0.0 633,072 + -0.5 633,072 + +1.2 633,072 + +1.0 633,072 + +1.1 633,073 - -0.4 633,073 - -0.4 633,073 - +0.7 633,073 - -0.2 633,073 - -1.5 633,073 - -1.2 633,073 - -0.6 633,073 - -1.6 633,073 - +0.2 633,073 - -0.2 633,073 - -1.2 633,073 - +0.0 633,073 - -0.8 633,073 - +1.1 633,073 - -1.3 633,258 + GFF1302 0.49 +0.1 633,258 + GFF1302 0.49 -0.7 633,258 + GFF1302 0.49 +0.2 633,258 + GFF1302 0.49 -0.6 633,259 - GFF1302 0.49 +0.2 633,259 - GFF1302 0.49 -0.8 633,259 - GFF1302 0.49 -0.9 633,259 - GFF1302 0.49 -0.7 633,303 + GFF1302 0.60 +0.6 633,303 + GFF1302 0.60 +0.4 633,303 + GFF1302 0.60 -0.4 633,303 + GFF1302 0.60 -1.8 633,303 + GFF1302 0.60 -1.8 633,303 + GFF1302 0.60 -0.6 633,303 + GFF1302 0.60 -0.1 633,304 - GFF1302 0.60 +0.4 633,304 - GFF1302 0.60 -0.2 633,304 - GFF1302 0.60 +0.3 633,304 - GFF1302 0.60 -0.2 633,304 - GFF1302 0.60 +0.3 633,304 - GFF1302 0.60 +0.4 633,312 + GFF1302 0.62 -0.6 633,313 - GFF1302 0.63 +0.7 633,313 - GFF1302 0.63 -0.4 633,313 - GFF1302 0.63 +1.1 633,466 + +0.4 633,467 - +1.1 633,467 - -0.2 633,467 - -2.9 633,467 - +0.1 633,496 + -0.2 633,496 + -1.0 633,496 + +1.4 633,497 - -0.6 633,497 - -0.1 633,501 + +1.1 633,501 + +0.4 633,502 - +0.3 633,502 - -0.3 633,502 - +1.1 633,529 + -0.3 633,529 + +0.2 633,529 + +0.0 633,529 + -0.7 633,529 + -1.9 633,529 + -1.6 633,529 + -0.4 633,529 + +0.6 633,529 + +0.0 633,529 + -0.7 633,529 + +0.4 633,529 + -0.9 633,529 + -0.0 633,529 + -0.8 633,529 + -0.9 633,529 + +0.4 633,529 + -0.7 633,529 + -0.7 633,529 + +0.7 633,529 + -0.9 633,529 + -0.1 633,529 + -0.4 633,529 + +0.6 633,529 + -0.2 633,529 + -0.3 633,529 + -0.6 633,530 - -0.5 633,530 - -0.6 633,530 - -0.6 633,530 - +0.5 633,530 - +1.2 633,530 - +0.5 633,530 - -1.2 633,530 - -2.1 633,530 - +1.4 633,530 - +0.2 633,530 - -0.2 633,530 - -1.1 633,530 - -1.6 633,530 - -1.1 633,530 - -0.7 633,530 - -0.3 633,530 - +0.1 633,530 - -0.5 633,530 - -1.9 633,533 + -0.3 633,533 + +0.4 633,533 + -0.0 633,533 + -1.2 633,533 + -1.6 633,533 + -0.4 633,533 + -0.9 633,533 + -2.3 633,533 + +0.1 633,533 + +1.7 633,534 - +0.4 633,534 - -0.9 633,534 - -1.0 633,534 - -0.6 633,534 - -0.0 633,534 - -1.4 633,534 - -0.4 633,534 - +0.8 633,534 - +0.2 633,575 + +0.6 633,575 + -0.6 633,575 + -0.1 633,575 + -1.8 633,575 + -0.6 633,575 + -1.0 633,576 - -0.6 633,576 - -0.9 633,576 - -0.0 633,576 - +0.9 633,576 - -0.4 633,576 - -0.4 633,576 - -0.9 633,576 - +0.5 633,576 - +0.6 633,576 - +0.4 633,591 + -2.1 633,591 + +0.4 633,592 - -0.9 633,989 + GFF1303 0.36 -0.9 633,989 + GFF1303 0.36 +0.4 633,989 + GFF1303 0.36 +0.4 633,989 + GFF1303 0.36 +1.8 633,989 + GFF1303 0.36 +1.1 633,989 + GFF1303 0.36 -0.4 633,989 + GFF1303 0.36 -1.9 633,989 + GFF1303 0.36 +1.8 633,990 - GFF1303 0.37 -0.2 633,990 - GFF1303 0.37 -0.4 633,990 - GFF1303 0.37 +0.4 633,990 - GFF1303 0.37 +1.6 633,990 - GFF1303 0.37 +0.4 633,990 - GFF1303 0.37 -1.0 633,992 + GFF1303 0.37 -0.3 633,993 - GFF1303 0.37 -0.2 633,993 - GFF1303 0.37 +0.3 633,993 - GFF1303 0.37 +0.9 633,993 - GFF1303 0.37 +1.2 634,109 + GFF1303 0.48 -2.8 634,110 - GFF1303 0.48 -1.2 634,110 - GFF1303 0.48 +0.6 634,110 - GFF1303 0.48 +1.1 634,265 + GFF1303 0.63 -0.5 634,266 - GFF1303 0.63 +0.6 634,266 - GFF1303 0.63 -1.2 634,266 - GFF1303 0.63 +0.4 634,266 - GFF1303 0.63 +0.2 634,266 - GFF1303 0.63 +1.8 634,535 + GFF1303 0.89 -0.5 634,535 + GFF1303 0.89 +0.0 634,535 + GFF1303 0.89 +1.4 634,535 + GFF1303 0.89 -2.0 634,536 - GFF1303 0.89 -0.2 634,536 - GFF1303 0.89 -1.5 634,628 + +1.0 634,628 + -0.2 634,628 + -1.8 634,628 + -0.6 634,628 + -0.2 634,628 + -0.4 634,628 + +0.2 634,628 + -0.1 634,628 + -1.2 634,628 + +0.1 634,628 + -0.4 634,629 - +0.0 634,629 - -1.1 634,629 - +0.6 634,629 - -0.2 634,629 - +0.4 634,629 - -0.0 634,629 - -0.3 634,629 - +0.7 634,629 - -0.3 634,629 - -0.3 634,629 - -1.2 634,629 - +1.1 634,629 - +1.0 634,629 - -1.4 634,629 - +0.1 634,629 - -0.0 634,629 - +0.0 634,629 - -1.0 635,145 + GFF1304 0.36 -2.1 635,145 + GFF1304 0.36 -0.5 635,145 + GFF1304 0.36 -2.2 635,146 - GFF1304 0.36 -0.4 635,146 - GFF1304 0.36 -0.1 635,146 - GFF1304 0.36 -0.4 635,146 - GFF1304 0.36 -0.6 635,146 - GFF1304 0.36 +0.2 635,146 - GFF1304 0.36 -0.4 635,321 + GFF1304 0.53 -0.4 635,321 + GFF1304 0.53 -0.3 635,322 - GFF1304 0.54 -1.4 635,322 - GFF1304 0.54 +0.4 635,349 - GFF1304 0.56 +1.0 635,349 - GFF1304 0.56 -1.9 635,388 + GFF1304 0.60 -1.1 635,388 + GFF1304 0.60 +0.4 635,388 + GFF1304 0.60 -0.3 635,388 + GFF1304 0.60 -1.9 635,389 - GFF1304 0.60 -0.6 635,389 - GFF1304 0.60 +2.0 635,389 - GFF1304 0.60 -0.9 635,389 - GFF1304 0.60 -0.9 635,389 - GFF1304 0.60 -0.5 635,416 + GFF1304 0.63 -0.8 635,419 - GFF1304 0.63 -0.4 635,419 - GFF1304 0.63 -1.0
Or see this region's nucleotide sequence