Strain Fitness in Variovorax sp. SCN45 around GFF1160

Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntGFF1158 and GFF1159 are separated by 104 nucleotidesGFF1159 and GFF1160 are separated by 53 nucleotidesGFF1160 and GFF1161 are separated by 53 nucleotides GFF1158 - Acetolactate synthase large subunit (EC 2.2.1.6), at 473,270 to 475,054 GFF1158 GFF1159 - Acetolactate synthase small subunit (EC 2.2.1.6), at 475,159 to 475,650 GFF1159 GFF1160 - hypothetical protein, at 475,704 to 475,967 GFF1160 GFF1161 - Ketol-acid reductoisomerase (NADP(+)) (EC 1.1.1.86), at 476,021 to 477,037 GFF1161 Position (kb) 475 476Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 474.827 kb on + strand, within GFF1158at 475.081 kb on - strandat 475.081 kb on - strandat 475.092 kb on + strandat 475.092 kb on + strandat 475.092 kb on + strandat 475.092 kb on + strandat 475.093 kb on - strandat 475.093 kb on - strandat 475.093 kb on - strandat 475.093 kb on - strandat 475.115 kb on + strandat 475.116 kb on - strandat 475.240 kb on + strand, within GFF1159at 475.240 kb on + strand, within GFF1159at 475.240 kb on + strand, within GFF1159at 475.240 kb on + strand, within GFF1159at 475.240 kb on + strand, within GFF1159at 475.240 kb on + strand, within GFF1159at 475.241 kb on - strand, within GFF1159at 475.241 kb on - strand, within GFF1159at 475.399 kb on + strand, within GFF1159at 475.516 kb on + strand, within GFF1159at 475.516 kb on + strand, within GFF1159at 475.516 kb on + strand, within GFF1159at 475.516 kb on + strand, within GFF1159at 475.516 kb on + strand, within GFF1159at 475.516 kb on + strand, within GFF1159at 475.516 kb on + strand, within GFF1159at 475.516 kb on + strand, within GFF1159at 475.516 kb on + strand, within GFF1159at 475.516 kb on + strand, within GFF1159at 475.516 kb on + strand, within GFF1159at 475.516 kb on + strand, within GFF1159at 475.517 kb on - strand, within GFF1159at 475.517 kb on - strand, within GFF1159at 475.517 kb on - strand, within GFF1159at 475.517 kb on - strand, within GFF1159at 475.517 kb on - strand, within GFF1159at 475.578 kb on + strand, within GFF1159at 475.579 kb on - strand, within GFF1159at 475.579 kb on - strand, within GFF1159at 475.579 kb on - strand, within GFF1159at 475.585 kb on - strand, within GFF1159at 475.585 kb on - strand, within GFF1159at 475.648 kb on + strandat 475.761 kb on + strand, within GFF1160at 475.761 kb on + strand, within GFF1160at 475.761 kb on + strand, within GFF1160at 475.761 kb on + strand, within GFF1160at 475.761 kb on + strand, within GFF1160at 475.761 kb on + strand, within GFF1160at 475.761 kb on + strand, within GFF1160at 475.761 kb on + strand, within GFF1160at 475.761 kb on + strand, within GFF1160at 475.761 kb on + strand, within GFF1160at 475.761 kb on + strand, within GFF1160at 475.761 kb on + strand, within GFF1160at 475.761 kb on + strand, within GFF1160at 475.762 kb on - strand, within GFF1160at 476.003 kb on + strandat 476.003 kb on + strandat 476.003 kb on + strandat 476.007 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
remove
474,827 + GFF1158 0.87 -1.2
475,081 - -0.7
475,081 - +0.1
475,092 + +1.2
475,092 + -0.9
475,092 + +1.2
475,092 + -0.5
475,093 - +0.1
475,093 - -0.5
475,093 - -1.9
475,093 - -1.9
475,115 + -0.4
475,116 - +0.7
475,240 + GFF1159 0.16 -0.7
475,240 + GFF1159 0.16 +0.6
475,240 + GFF1159 0.16 +0.2
475,240 + GFF1159 0.16 -0.1
475,240 + GFF1159 0.16 -0.6
475,240 + GFF1159 0.16 -2.8
475,241 - GFF1159 0.17 +0.1
475,241 - GFF1159 0.17 -1.9
475,399 + GFF1159 0.49 +0.3
475,516 + GFF1159 0.73 -0.2
475,516 + GFF1159 0.73 -1.2
475,516 + GFF1159 0.73 -0.7
475,516 + GFF1159 0.73 -0.6
475,516 + GFF1159 0.73 -0.2
475,516 + GFF1159 0.73 -0.7
475,516 + GFF1159 0.73 -0.5
475,516 + GFF1159 0.73 -2.5
475,516 + GFF1159 0.73 +0.4
475,516 + GFF1159 0.73 +0.8
475,516 + GFF1159 0.73 -0.2
475,516 + GFF1159 0.73 +0.2
475,517 - GFF1159 0.73 -1.4
475,517 - GFF1159 0.73 -1.9
475,517 - GFF1159 0.73 -0.6
475,517 - GFF1159 0.73 -1.4
475,517 - GFF1159 0.73 +0.1
475,578 + GFF1159 0.85 +1.2
475,579 - GFF1159 0.85 +0.7
475,579 - GFF1159 0.85 +0.9
475,579 - GFF1159 0.85 -1.0
475,585 - GFF1159 0.87 -1.6
475,585 - GFF1159 0.87 -1.5
475,648 + -0.6
475,761 + GFF1160 0.22 -0.3
475,761 + GFF1160 0.22 +1.3
475,761 + GFF1160 0.22 -1.1
475,761 + GFF1160 0.22 -0.9
475,761 + GFF1160 0.22 -0.2
475,761 + GFF1160 0.22 -1.2
475,761 + GFF1160 0.22 +0.0
475,761 + GFF1160 0.22 -0.5
475,761 + GFF1160 0.22 -0.6
475,761 + GFF1160 0.22 +2.4
475,761 + GFF1160 0.22 -1.9
475,761 + GFF1160 0.22 -0.6
475,761 + GFF1160 0.22 -0.6
475,762 - GFF1160 0.22 +2.0
476,003 + -1.1
476,003 + -0.4
476,003 + +0.1
476,007 + +0.7

Or see this region's nucleotide sequence