Experiment: Lentilan from mushroom 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1181_RS16980 and HMPREF1181_RS16985 are separated by 84 nucleotides HMPREF1181_RS16985 and HMPREF1181_RS16990 are separated by 134 nucleotides
HMPREF1181_RS16980: HMPREF1181_RS16980 - D-2-hydroxyacid dehydrogenase, at 113,712 to 114,668
_RS16980
HMPREF1181_RS16985: HMPREF1181_RS16985 - endonuclease/exonuclease/phosphatase family protein, at 114,753 to 115,865
_RS16985
HMPREF1181_RS16990: HMPREF1181_RS16990 - TonB-dependent receptor, at 116,000 to 119,194
_RS16990
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-2
-1
0
1 at 113.792 kb on + strand at 113.937 kb on + strand, within HMPREF1181_RS16980 at 114.001 kb on - strand, within HMPREF1181_RS16980 at 114.070 kb on - strand, within HMPREF1181_RS16980 at 114.083 kb on - strand, within HMPREF1181_RS16980 at 114.190 kb on - strand, within HMPREF1181_RS16980 at 114.230 kb on - strand, within HMPREF1181_RS16980 at 114.237 kb on + strand, within HMPREF1181_RS16980 at 114.238 kb on - strand, within HMPREF1181_RS16980 at 114.238 kb on - strand, within HMPREF1181_RS16980 at 114.238 kb on - strand, within HMPREF1181_RS16980 at 114.621 kb on - strand at 114.623 kb on - strand at 114.730 kb on + strand at 114.748 kb on + strand at 114.797 kb on - strand at 114.814 kb on + strand at 114.852 kb on + strand at 115.042 kb on + strand, within HMPREF1181_RS16985 at 115.119 kb on - strand, within HMPREF1181_RS16985 at 115.167 kb on + strand, within HMPREF1181_RS16985 at 115.168 kb on - strand, within HMPREF1181_RS16985 at 115.363 kb on - strand, within HMPREF1181_RS16985 at 115.410 kb on - strand, within HMPREF1181_RS16985 at 115.449 kb on - strand, within HMPREF1181_RS16985 at 115.660 kb on - strand, within HMPREF1181_RS16985 at 115.725 kb on + strand, within HMPREF1181_RS16985 at 115.725 kb on + strand, within HMPREF1181_RS16985 at 115.732 kb on - strand, within HMPREF1181_RS16985 at 115.754 kb on - strand at 115.801 kb on - strand at 115.803 kb on + strand at 115.822 kb on + strand at 115.847 kb on - strand at 115.876 kb on + strand at 115.968 kb on + strand at 115.979 kb on + strand at 115.980 kb on - strand at 115.995 kb on + strand at 116.077 kb on - strand at 116.081 kb on - strand at 116.082 kb on + strand at 116.082 kb on + strand at 116.086 kb on + strand at 116.086 kb on + strand at 116.157 kb on + strand at 116.158 kb on - strand at 116.243 kb on - strand at 116.243 kb on - strand at 116.243 kb on - strand at 116.244 kb on + strand at 116.244 kb on + strand at 116.245 kb on - strand at 116.245 kb on - strand at 116.245 kb on - strand at 116.245 kb on - strand at 116.337 kb on + strand, within HMPREF1181_RS16990 at 116.462 kb on - strand, within HMPREF1181_RS16990 at 116.476 kb on + strand, within HMPREF1181_RS16990 at 116.554 kb on - strand, within HMPREF1181_RS16990 at 116.605 kb on + strand, within HMPREF1181_RS16990 at 116.606 kb on - strand, within HMPREF1181_RS16990 at 116.608 kb on - strand, within HMPREF1181_RS16990 at 116.657 kb on + strand, within HMPREF1181_RS16990 at 116.658 kb on - strand, within HMPREF1181_RS16990 at 116.658 kb on - strand, within HMPREF1181_RS16990 at 116.658 kb on - strand, within HMPREF1181_RS16990 at 116.695 kb on - strand, within HMPREF1181_RS16990 at 116.820 kb on + strand, within HMPREF1181_RS16990 at 116.821 kb on - strand, within HMPREF1181_RS16990 at 116.821 kb on - strand, within HMPREF1181_RS16990 at 116.824 kb on + strand, within HMPREF1181_RS16990 at 116.825 kb on - strand, within HMPREF1181_RS16990 at 116.825 kb on - strand, within HMPREF1181_RS16990 at 116.825 kb on - strand, within HMPREF1181_RS16990 at 116.865 kb on + strand, within HMPREF1181_RS16990
Per-strain Table
Position Strand Gene LocusTag Fraction Lentilan from mushroom 2.5 mg/ml (C) remove 113,792 + +1.1 113,937 + HMPREF1181_RS16980 0.24 +1.6 114,001 - HMPREF1181_RS16980 0.30 +0.2 114,070 - HMPREF1181_RS16980 0.37 +0.5 114,083 - HMPREF1181_RS16980 0.39 +0.7 114,190 - HMPREF1181_RS16980 0.50 +1.6 114,230 - HMPREF1181_RS16980 0.54 +1.0 114,237 + HMPREF1181_RS16980 0.55 +1.4 114,238 - HMPREF1181_RS16980 0.55 -0.1 114,238 - HMPREF1181_RS16980 0.55 -0.1 114,238 - HMPREF1181_RS16980 0.55 +1.0 114,621 - +1.5 114,623 - +0.4 114,730 + +0.8 114,748 + -0.1 114,797 - -0.2 114,814 + -1.4 114,852 + -0.7 115,042 + HMPREF1181_RS16985 0.26 +0.4 115,119 - HMPREF1181_RS16985 0.33 +0.7 115,167 + HMPREF1181_RS16985 0.37 -0.1 115,168 - HMPREF1181_RS16985 0.37 +0.7 115,363 - HMPREF1181_RS16985 0.55 -1.4 115,410 - HMPREF1181_RS16985 0.59 +0.0 115,449 - HMPREF1181_RS16985 0.63 +0.9 115,660 - HMPREF1181_RS16985 0.81 +0.1 115,725 + HMPREF1181_RS16985 0.87 -0.2 115,725 + HMPREF1181_RS16985 0.87 -0.1 115,732 - HMPREF1181_RS16985 0.88 -0.8 115,754 - -0.1 115,801 - -0.9 115,803 + -0.6 115,822 + +0.9 115,847 - -0.1 115,876 + -0.2 115,968 + +0.2 115,979 + -2.0 115,980 - -0.2 115,995 + +0.1 116,077 - -0.4 116,081 - +0.5 116,082 + -0.5 116,082 + +0.0 116,086 + +0.8 116,086 + +0.5 116,157 + +0.4 116,158 - -0.1 116,243 - -0.1 116,243 - -0.5 116,243 - +0.4 116,244 + +0.1 116,244 + +0.0 116,245 - -0.1 116,245 - -0.2 116,245 - -0.1 116,245 - -0.2 116,337 + HMPREF1181_RS16990 0.11 -1.0 116,462 - HMPREF1181_RS16990 0.14 -0.2 116,476 + HMPREF1181_RS16990 0.15 +0.3 116,554 - HMPREF1181_RS16990 0.17 +0.5 116,605 + HMPREF1181_RS16990 0.19 +0.1 116,606 - HMPREF1181_RS16990 0.19 +0.1 116,608 - HMPREF1181_RS16990 0.19 -0.5 116,657 + HMPREF1181_RS16990 0.21 -0.1 116,658 - HMPREF1181_RS16990 0.21 -0.8 116,658 - HMPREF1181_RS16990 0.21 +0.3 116,658 - HMPREF1181_RS16990 0.21 -0.5 116,695 - HMPREF1181_RS16990 0.22 -1.7 116,820 + HMPREF1181_RS16990 0.26 +0.6 116,821 - HMPREF1181_RS16990 0.26 -1.1 116,821 - HMPREF1181_RS16990 0.26 -0.4 116,824 + HMPREF1181_RS16990 0.26 +0.0 116,825 - HMPREF1181_RS16990 0.26 +0.5 116,825 - HMPREF1181_RS16990 0.26 +0.6 116,825 - HMPREF1181_RS16990 0.26 +0.1 116,865 + HMPREF1181_RS16990 0.27 +0.3
Or see this region's nucleotide sequence