Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF928 and GFF929 are separated by 160 nucleotides GFF929 and GFF930 are separated by 27 nucleotides GFF930 and GFF931 are separated by 128 nucleotides
GFF928 - tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.91), at 240,650 to 241,630
GFF928
GFF929 - Transcriptional regulator, IclR family, at 241,791 to 242,579
GFF929
GFF930 - Transcriptional regulator, GntR family, at 242,607 to 243,248
GFF930
GFF931 - Methylmalonyl-CoA mutase (EC 5.4.99.2) / B12 binding domain of Methylmalonyl-CoA mutase (EC 5.4.99.2), at 243,377 to 245,539
GFF931
Position (kb)
241
242
243 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 240.795 kb on + strand, within GFF928 at 240.795 kb on + strand, within GFF928 at 240.796 kb on - strand, within GFF928 at 240.796 kb on - strand, within GFF928 at 240.905 kb on + strand, within GFF928 at 240.905 kb on + strand, within GFF928 at 240.905 kb on + strand, within GFF928 at 240.906 kb on - strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.083 kb on - strand, within GFF928 at 241.083 kb on - strand, within GFF928 at 241.083 kb on - strand, within GFF928 at 241.211 kb on + strand, within GFF928 at 241.343 kb on + strand, within GFF928 at 241.343 kb on + strand, within GFF928 at 241.343 kb on + strand, within GFF928 at 241.343 kb on + strand, within GFF928 at 241.344 kb on - strand, within GFF928 at 241.344 kb on - strand, within GFF928 at 241.385 kb on + strand, within GFF928 at 241.386 kb on - strand, within GFF928 at 241.386 kb on - strand, within GFF928 at 241.386 kb on - strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.712 kb on + strand at 241.712 kb on + strand at 241.722 kb on + strand at 241.722 kb on + strand at 241.722 kb on + strand at 241.722 kb on + strand at 241.722 kb on + strand at 241.723 kb on - strand at 241.723 kb on - strand at 241.723 kb on - strand at 241.723 kb on - strand at 241.773 kb on + strand at 241.773 kb on + strand at 241.773 kb on + strand at 241.773 kb on + strand at 241.773 kb on + strand at 241.773 kb on + strand at 241.773 kb on + strand at 241.773 kb on + strand at 241.773 kb on + strand at 241.773 kb on + strand at 241.773 kb on + strand at 241.774 kb on - strand at 241.774 kb on - strand at 241.774 kb on - strand at 241.774 kb on - strand at 241.774 kb on - strand at 241.774 kb on - strand at 241.788 kb on + strand at 241.789 kb on - strand at 241.882 kb on + strand, within GFF929 at 241.882 kb on + strand, within GFF929 at 241.883 kb on - strand, within GFF929 at 241.883 kb on - strand, within GFF929 at 241.946 kb on + strand, within GFF929 at 241.946 kb on + strand, within GFF929 at 241.965 kb on + strand, within GFF929 at 241.965 kb on + strand, within GFF929 at 241.965 kb on + strand, within GFF929 at 242.021 kb on + strand, within GFF929 at 242.021 kb on + strand, within GFF929 at 242.022 kb on - strand, within GFF929 at 242.034 kb on + strand, within GFF929 at 242.034 kb on + strand, within GFF929 at 242.034 kb on + strand, within GFF929 at 242.034 kb on + strand, within GFF929 at 242.035 kb on - strand, within GFF929 at 242.035 kb on - strand, within GFF929 at 242.213 kb on + strand, within GFF929 at 242.214 kb on - strand, within GFF929 at 242.355 kb on + strand, within GFF929 at 242.355 kb on + strand, within GFF929 at 242.355 kb on + strand, within GFF929 at 242.356 kb on - strand, within GFF929 at 242.356 kb on - strand, within GFF929 at 242.371 kb on - strand, within GFF929 at 242.371 kb on - strand, within GFF929 at 242.413 kb on + strand, within GFF929 at 242.413 kb on + strand, within GFF929 at 242.413 kb on + strand, within GFF929 at 242.413 kb on + strand, within GFF929 at 242.413 kb on + strand, within GFF929 at 242.413 kb on + strand, within GFF929 at 242.413 kb on + strand, within GFF929 at 242.414 kb on - strand, within GFF929 at 242.448 kb on + strand, within GFF929 at 242.448 kb on + strand, within GFF929 at 242.449 kb on - strand, within GFF929 at 242.449 kb on - strand, within GFF929 at 242.449 kb on - strand, within GFF929 at 242.475 kb on + strand, within GFF929 at 242.475 kb on + strand, within GFF929 at 242.475 kb on + strand, within GFF929 at 242.475 kb on + strand, within GFF929 at 242.476 kb on - strand, within GFF929 at 242.476 kb on - strand, within GFF929 at 242.476 kb on - strand, within GFF929 at 242.608 kb on + strand at 242.608 kb on + strand at 242.608 kb on + strand at 242.608 kb on + strand at 242.609 kb on - strand at 242.609 kb on - strand at 242.995 kb on + strand, within GFF930 at 242.995 kb on + strand, within GFF930 at 242.995 kb on + strand, within GFF930 at 242.995 kb on + strand, within GFF930 at 242.995 kb on + strand, within GFF930 at 242.996 kb on - strand, within GFF930 at 242.996 kb on - strand, within GFF930 at 242.996 kb on - strand, within GFF930 at 242.996 kb on - strand, within GFF930 at 243.034 kb on + strand, within GFF930 at 243.034 kb on + strand, within GFF930 at 243.034 kb on + strand, within GFF930 at 243.034 kb on + strand, within GFF930 at 243.035 kb on - strand, within GFF930 at 243.035 kb on - strand, within GFF930 at 243.081 kb on + strand, within GFF930 at 243.081 kb on + strand, within GFF930 at 243.081 kb on + strand, within GFF930 at 243.082 kb on - strand, within GFF930 at 243.082 kb on - strand, within GFF930 at 243.082 kb on - strand, within GFF930 at 243.082 kb on - strand, within GFF930 at 243.082 kb on - strand, within GFF930 at 243.122 kb on - strand, within GFF930 at 243.122 kb on - strand, within GFF930 at 243.122 kb on - strand, within GFF930 at 243.214 kb on + strand at 243.214 kb on + strand at 243.214 kb on + strand at 243.214 kb on + strand at 243.215 kb on - strand at 243.215 kb on - strand at 243.215 kb on - strand at 243.215 kb on - strand at 243.215 kb on - strand at 243.230 kb on + strand at 243.231 kb on - strand at 243.231 kb on - strand at 243.260 kb on + strand at 243.260 kb on + strand at 243.260 kb on + strand at 243.260 kb on + strand at 243.260 kb on + strand at 243.260 kb on + strand at 243.260 kb on + strand at 243.261 kb on - strand at 243.261 kb on - strand at 243.261 kb on - strand at 243.290 kb on - strand at 243.300 kb on + strand at 243.300 kb on + strand at 243.300 kb on + strand at 243.300 kb on + strand at 243.301 kb on - strand at 243.301 kb on - strand at 243.345 kb on + strand at 243.345 kb on + strand at 243.345 kb on + strand at 243.345 kb on + strand at 243.345 kb on + strand at 243.345 kb on + strand at 243.345 kb on + strand at 243.345 kb on + strand at 243.346 kb on - strand at 243.346 kb on - strand at 243.346 kb on - strand at 243.346 kb on - strand at 243.515 kb on + strand at 243.515 kb on + strand at 243.515 kb on + strand at 243.545 kb on + strand at 243.545 kb on + strand at 243.545 kb on + strand at 243.545 kb on + strand at 243.545 kb on + strand at 243.575 kb on + strand at 243.575 kb on + strand at 243.575 kb on + strand at 243.575 kb on + strand at 243.575 kb on + strand at 243.576 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 240,795 + GFF928 0.15 -1.7 240,795 + GFF928 0.15 -0.7 240,796 - GFF928 0.15 +0.3 240,796 - GFF928 0.15 -2.1 240,905 + GFF928 0.26 -0.3 240,905 + GFF928 0.26 -1.2 240,905 + GFF928 0.26 -0.5 240,906 - GFF928 0.26 +0.2 241,082 + GFF928 0.44 -0.7 241,082 + GFF928 0.44 +0.2 241,082 + GFF928 0.44 +0.0 241,082 + GFF928 0.44 -0.6 241,082 + GFF928 0.44 -0.6 241,082 + GFF928 0.44 -1.4 241,082 + GFF928 0.44 -1.0 241,082 + GFF928 0.44 +0.8 241,083 - GFF928 0.44 -0.8 241,083 - GFF928 0.44 +0.3 241,083 - GFF928 0.44 -0.5 241,211 + GFF928 0.57 -0.3 241,343 + GFF928 0.71 -0.4 241,343 + GFF928 0.71 +0.3 241,343 + GFF928 0.71 +0.9 241,343 + GFF928 0.71 +0.1 241,344 - GFF928 0.71 -1.1 241,344 - GFF928 0.71 -0.3 241,385 + GFF928 0.75 -0.2 241,386 - GFF928 0.75 +0.2 241,386 - GFF928 0.75 -1.6 241,386 - GFF928 0.75 +1.0 241,442 + GFF928 0.81 -0.6 241,442 + GFF928 0.81 +0.2 241,442 + GFF928 0.81 +0.2 241,442 + GFF928 0.81 -0.8 241,442 + GFF928 0.81 -0.4 241,442 + GFF928 0.81 -0.9 241,442 + GFF928 0.81 -0.2 241,443 - GFF928 0.81 -2.2 241,443 - GFF928 0.81 +0.9 241,443 - GFF928 0.81 +0.0 241,443 - GFF928 0.81 +0.0 241,443 - GFF928 0.81 -0.5 241,443 - GFF928 0.81 -0.5 241,443 - GFF928 0.81 +2.6 241,443 - GFF928 0.81 +0.4 241,443 - GFF928 0.81 -0.0 241,443 - GFF928 0.81 -0.6 241,712 + +1.3 241,712 + +0.0 241,722 + +0.4 241,722 + +0.1 241,722 + +0.0 241,722 + -1.2 241,722 + -0.5 241,723 - +2.0 241,723 - -0.4 241,723 - +0.9 241,723 - +0.6 241,773 + -0.1 241,773 + -0.8 241,773 + -0.8 241,773 + -0.6 241,773 + +0.7 241,773 + +0.6 241,773 + -1.0 241,773 + -0.2 241,773 + -2.3 241,773 + -0.7 241,773 + -1.4 241,774 - +0.1 241,774 - -1.6 241,774 - -0.3 241,774 - -1.3 241,774 - -1.0 241,774 - +0.6 241,788 + -0.7 241,789 - -0.6 241,882 + GFF929 0.12 -0.4 241,882 + GFF929 0.12 +0.0 241,883 - GFF929 0.12 +0.6 241,883 - GFF929 0.12 +0.3 241,946 + GFF929 0.20 +1.3 241,946 + GFF929 0.20 +0.9 241,965 + GFF929 0.22 +0.3 241,965 + GFF929 0.22 +0.6 241,965 + GFF929 0.22 -2.2 242,021 + GFF929 0.29 +0.1 242,021 + GFF929 0.29 -1.9 242,022 - GFF929 0.29 +0.1 242,034 + GFF929 0.31 -0.4 242,034 + GFF929 0.31 -0.2 242,034 + GFF929 0.31 -0.2 242,034 + GFF929 0.31 -0.3 242,035 - GFF929 0.31 -0.2 242,035 - GFF929 0.31 +0.9 242,213 + GFF929 0.53 -0.1 242,214 - GFF929 0.54 +0.4 242,355 + GFF929 0.71 +0.3 242,355 + GFF929 0.71 -0.3 242,355 + GFF929 0.71 +0.7 242,356 - GFF929 0.72 +1.6 242,356 - GFF929 0.72 +0.2 242,371 - GFF929 0.74 -0.4 242,371 - GFF929 0.74 -0.7 242,413 + GFF929 0.79 +0.0 242,413 + GFF929 0.79 -0.3 242,413 + GFF929 0.79 +0.8 242,413 + GFF929 0.79 -0.4 242,413 + GFF929 0.79 -0.2 242,413 + GFF929 0.79 -0.1 242,413 + GFF929 0.79 -1.7 242,414 - GFF929 0.79 +0.4 242,448 + GFF929 0.83 -0.6 242,448 + GFF929 0.83 -0.4 242,449 - GFF929 0.83 +0.1 242,449 - GFF929 0.83 +0.8 242,449 - GFF929 0.83 +0.8 242,475 + GFF929 0.87 -1.2 242,475 + GFF929 0.87 +0.8 242,475 + GFF929 0.87 -0.3 242,475 + GFF929 0.87 -1.6 242,476 - GFF929 0.87 +0.6 242,476 - GFF929 0.87 -0.3 242,476 - GFF929 0.87 -1.5 242,608 + -1.2 242,608 + +1.0 242,608 + +0.3 242,608 + +0.2 242,609 - +0.0 242,609 - +0.4 242,995 + GFF930 0.60 -0.1 242,995 + GFF930 0.60 -2.4 242,995 + GFF930 0.60 -0.9 242,995 + GFF930 0.60 +0.5 242,995 + GFF930 0.60 +1.4 242,996 - GFF930 0.61 -0.3 242,996 - GFF930 0.61 -0.6 242,996 - GFF930 0.61 -1.2 242,996 - GFF930 0.61 +2.0 243,034 + GFF930 0.67 -0.6 243,034 + GFF930 0.67 +0.2 243,034 + GFF930 0.67 -0.4 243,034 + GFF930 0.67 -0.9 243,035 - GFF930 0.67 +0.6 243,035 - GFF930 0.67 -1.0 243,081 + GFF930 0.74 +0.2 243,081 + GFF930 0.74 -0.8 243,081 + GFF930 0.74 -1.7 243,082 - GFF930 0.74 +1.6 243,082 - GFF930 0.74 +3.9 243,082 - GFF930 0.74 -1.2 243,082 - GFF930 0.74 -0.3 243,082 - GFF930 0.74 -1.0 243,122 - GFF930 0.80 +0.5 243,122 - GFF930 0.80 -0.2 243,122 - GFF930 0.80 -0.4 243,214 + -0.4 243,214 + -1.0 243,214 + -0.8 243,214 + +0.0 243,215 - -0.6 243,215 - +0.6 243,215 - +0.5 243,215 - -1.3 243,215 - -0.5 243,230 + -0.1 243,231 - -0.2 243,231 - -0.6 243,260 + +0.2 243,260 + -1.0 243,260 + -0.0 243,260 + -0.7 243,260 + -0.4 243,260 + -1.1 243,260 + -1.0 243,261 - -0.7 243,261 - +0.3 243,261 - -1.3 243,290 - +0.4 243,300 + -0.9 243,300 + -0.4 243,300 + +1.0 243,300 + +0.5 243,301 - +0.8 243,301 - -0.1 243,345 + -0.1 243,345 + -0.1 243,345 + +0.1 243,345 + +0.1 243,345 + +0.3 243,345 + -0.6 243,345 + +0.2 243,345 + -0.1 243,346 - -0.9 243,346 - -0.7 243,346 - +0.1 243,346 - +2.2 243,515 + -0.6 243,515 + -0.9 243,515 + -0.8 243,545 + -0.1 243,545 + -0.6 243,545 + +0.3 243,545 + -1.6 243,545 + -0.7 243,575 + -0.6 243,575 + +0.2 243,575 + -0.7 243,575 + -1.2 243,575 + +0.2 243,576 - +0.1
Or see this region's nucleotide sequence