Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6981 and GFF6982 are separated by 7 nucleotides GFF6982 and GFF6983 are separated by 26 nucleotides GFF6983 and GFF6984 are separated by 34 nucleotides GFF6984 and GFF6985 are separated by 177 nucleotides
GFF6981 - hypothetical protein, at 381 to 956
GFF6981
GFF6982 - hypothetical protein, at 964 to 1,443
GFF6982
GFF6983 - FIG140336: TPR domain protein, at 1,470 to 2,747
GFF6983
GFF6984 - RNA polymerase sigma factor for flagellar operon, at 2,782 to 3,468
GFF6984
GFF6985 - Flagellar biosynthesis protein FliP, at 3,646 to 4,419
GFF6985
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-2
-1
0
1 at 0.621 kb on + strand, within GFF6981 at 0.674 kb on + strand, within GFF6981 at 0.781 kb on - strand, within GFF6981 at 0.909 kb on - strand at 0.930 kb on + strand at 1.040 kb on + strand, within GFF6982 at 1.040 kb on + strand, within GFF6982 at 1.257 kb on - strand, within GFF6982 at 1.257 kb on - strand, within GFF6982 at 1.509 kb on + strand at 1.510 kb on - strand at 1.510 kb on - strand at 1.724 kb on - strand, within GFF6983 at 1.724 kb on - strand, within GFF6983 at 1.724 kb on - strand, within GFF6983 at 2.386 kb on + strand, within GFF6983 at 2.386 kb on + strand, within GFF6983 at 2.560 kb on + strand, within GFF6983 at 2.560 kb on + strand, within GFF6983 at 2.560 kb on + strand, within GFF6983 at 2.561 kb on - strand, within GFF6983 at 2.739 kb on - strand at 2.762 kb on + strand at 2.921 kb on + strand, within GFF6984 at 2.922 kb on - strand, within GFF6984 at 2.980 kb on + strand, within GFF6984 at 3.022 kb on + strand, within GFF6984 at 3.022 kb on + strand, within GFF6984 at 3.023 kb on - strand, within GFF6984 at 3.023 kb on - strand, within GFF6984 at 3.131 kb on + strand, within GFF6984 at 3.382 kb on + strand, within GFF6984 at 3.410 kb on + strand at 3.557 kb on - strand at 3.557 kb on - strand at 3.557 kb on - strand at 3.634 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 621 + GFF6981 0.42 +0.0 674 + GFF6981 0.51 -1.2 781 - GFF6981 0.69 +1.7 909 - +1.1 930 + -0.9 1,040 + GFF6982 0.16 +0.2 1,040 + GFF6982 0.16 +0.5 1,257 - GFF6982 0.61 -0.4 1,257 - GFF6982 0.61 +0.5 1,509 + +0.5 1,510 - -0.6 1,510 - -0.1 1,724 - GFF6983 0.20 -0.1 1,724 - GFF6983 0.20 -0.8 1,724 - GFF6983 0.20 -0.8 2,386 + GFF6983 0.72 -0.2 2,386 + GFF6983 0.72 -0.6 2,560 + GFF6983 0.85 -0.6 2,560 + GFF6983 0.85 -0.2 2,560 + GFF6983 0.85 -0.2 2,561 - GFF6983 0.85 +0.4 2,739 - -0.4 2,762 + -1.2 2,921 + GFF6984 0.20 -0.4 2,922 - GFF6984 0.20 -0.4 2,980 + GFF6984 0.29 -1.9 3,022 + GFF6984 0.35 -0.8 3,022 + GFF6984 0.35 -1.6 3,023 - GFF6984 0.35 +0.0 3,023 - GFF6984 0.35 -0.3 3,131 + GFF6984 0.51 -0.9 3,382 + GFF6984 0.87 +0.3 3,410 + -0.7 3,557 - +0.3 3,557 - -0.2 3,557 - -0.7 3,634 - -0.9
Or see this region's nucleotide sequence