Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6714 and GFF6715 are separated by 10 nucleotides GFF6715 and GFF6716 overlap by 8 nucleotides GFF6716 and GFF6717 are separated by 150 nucleotides
GFF6714 - Putative cryptic D-serine deaminase (EC 4.3.1.18), at 22,301 to 23,617
GFF6714
GFF6715 - Transcriptional regulator of RpiR family in catabolic operon, at 23,628 to 24,527
GFF6715
GFF6716 - N-acyl-D-amino-acid deacylase (EC 3.5.1.81), at 24,520 to 26,001
GFF6716
GFF6717 - hypothetical protein, at 26,152 to 28,392
GFF6717
Position (kb) 24
25
26
27 Strain fitness (log2 ratio) -2
-1
0
1
2 at 23.663 kb on - strand at 24.034 kb on - strand, within GFF6715 at 24.123 kb on + strand, within GFF6715 at 24.123 kb on + strand, within GFF6715 at 24.123 kb on + strand, within GFF6715 at 24.124 kb on - strand, within GFF6715 at 24.354 kb on + strand, within GFF6715 at 24.354 kb on + strand, within GFF6715 at 24.382 kb on + strand, within GFF6715 at 24.382 kb on + strand, within GFF6715 at 25.117 kb on + strand, within GFF6716 at 25.118 kb on - strand, within GFF6716 at 25.118 kb on - strand, within GFF6716 at 25.192 kb on + strand, within GFF6716 at 25.390 kb on + strand, within GFF6716 at 25.390 kb on + strand, within GFF6716 at 25.391 kb on - strand, within GFF6716 at 25.391 kb on - strand, within GFF6716 at 25.391 kb on - strand, within GFF6716 at 25.391 kb on - strand, within GFF6716 at 25.396 kb on + strand, within GFF6716 at 25.574 kb on - strand, within GFF6716 at 25.870 kb on + strand at 25.870 kb on + strand at 25.870 kb on + strand at 25.870 kb on + strand at 26.043 kb on + strand at 26.069 kb on + strand at 26.163 kb on + strand at 26.253 kb on + strand at 26.253 kb on + strand at 26.254 kb on - strand at 26.254 kb on - strand at 26.357 kb on - strand at 26.479 kb on + strand, within GFF6717 at 26.725 kb on + strand, within GFF6717 at 26.725 kb on + strand, within GFF6717 at 26.726 kb on - strand, within GFF6717 at 26.752 kb on + strand, within GFF6717 at 26.752 kb on + strand, within GFF6717 at 26.753 kb on - strand, within GFF6717 at 26.828 kb on + strand, within GFF6717 at 26.829 kb on - strand, within GFF6717 at 26.829 kb on - strand, within GFF6717 at 26.852 kb on - strand, within GFF6717 at 26.914 kb on + strand, within GFF6717 at 26.914 kb on + strand, within GFF6717
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 23,663 - -0.4 24,034 - GFF6715 0.45 -1.3 24,123 + GFF6715 0.55 +0.3 24,123 + GFF6715 0.55 +0.8 24,123 + GFF6715 0.55 +1.2 24,124 - GFF6715 0.55 +0.4 24,354 + GFF6715 0.81 -0.6 24,354 + GFF6715 0.81 -1.4 24,382 + GFF6715 0.84 +0.0 24,382 + GFF6715 0.84 -0.3 25,117 + GFF6716 0.40 -1.0 25,118 - GFF6716 0.40 +0.6 25,118 - GFF6716 0.40 -0.4 25,192 + GFF6716 0.45 +0.5 25,390 + GFF6716 0.59 +1.0 25,390 + GFF6716 0.59 +0.4 25,391 - GFF6716 0.59 -1.9 25,391 - GFF6716 0.59 +1.5 25,391 - GFF6716 0.59 -1.9 25,391 - GFF6716 0.59 +0.4 25,396 + GFF6716 0.59 +2.5 25,574 - GFF6716 0.71 +0.1 25,870 + -0.1 25,870 + -0.7 25,870 + -0.7 25,870 + +0.8 26,043 + -1.0 26,069 + -0.5 26,163 + -1.8 26,253 + +0.2 26,253 + -0.4 26,254 - -0.1 26,254 - -0.9 26,357 - +0.1 26,479 + GFF6717 0.15 -0.1 26,725 + GFF6717 0.26 -1.0 26,725 + GFF6717 0.26 -0.7 26,726 - GFF6717 0.26 -0.2 26,752 + GFF6717 0.27 +0.0 26,752 + GFF6717 0.27 +0.6 26,753 - GFF6717 0.27 -0.9 26,828 + GFF6717 0.30 +1.0 26,829 - GFF6717 0.30 +0.6 26,829 - GFF6717 0.30 +1.2 26,852 - GFF6717 0.31 +0.2 26,914 + GFF6717 0.34 -0.8 26,914 + GFF6717 0.34 -0.4
Or see this region's nucleotide sequence