Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6350 and GFF6351 are separated by 31 nucleotides GFF6351 and GFF6352 are separated by 1 nucleotides GFF6352 and GFF6353 overlap by 1 nucleotides
GFF6350 - T6SS Serine/threonine protein kinase (EC 2.7.11.1) PpkA, at 11,737 to 13,650
GFF6350
GFF6351 - T6SS associated component TagF (ImpM), at 13,682 to 14,395
GFF6351
GFF6352 - T6SS component TssM (IcmF/VasK), at 14,397 to 18,056
GFF6352
GFF6353 - T6SS outer membrane component TssL (ImpK/VasF) / OmpA/MotB domain, at 18,056 to 19,426
GFF6353
Position (kb) 14
15
16
17
18
19 Strain fitness (log2 ratio) -2
-1
0
1
2 at 13.463 kb on + strand at 13.463 kb on + strand at 13.463 kb on + strand at 13.463 kb on + strand at 13.463 kb on + strand at 13.463 kb on + strand at 13.463 kb on + strand at 13.464 kb on - strand at 13.464 kb on - strand at 13.464 kb on - strand at 13.464 kb on - strand at 13.464 kb on - strand at 13.503 kb on - strand at 13.797 kb on + strand, within GFF6351 at 13.879 kb on + strand, within GFF6351 at 13.879 kb on + strand, within GFF6351 at 14.025 kb on + strand, within GFF6351 at 14.025 kb on + strand, within GFF6351 at 14.062 kb on - strand, within GFF6351 at 14.103 kb on + strand, within GFF6351 at 14.205 kb on + strand, within GFF6351 at 14.206 kb on - strand, within GFF6351 at 14.206 kb on - strand, within GFF6351 at 14.452 kb on + strand at 14.452 kb on + strand at 14.452 kb on + strand at 14.452 kb on + strand at 14.453 kb on - strand at 14.702 kb on - strand at 14.860 kb on + strand, within GFF6352 at 15.277 kb on + strand, within GFF6352 at 15.277 kb on + strand, within GFF6352 at 15.278 kb on - strand, within GFF6352 at 15.278 kb on - strand, within GFF6352 at 15.673 kb on + strand, within GFF6352 at 15.674 kb on - strand, within GFF6352 at 15.960 kb on + strand, within GFF6352 at 15.960 kb on + strand, within GFF6352 at 15.961 kb on - strand, within GFF6352 at 15.980 kb on - strand, within GFF6352 at 16.216 kb on + strand, within GFF6352 at 16.235 kb on - strand, within GFF6352 at 16.235 kb on - strand, within GFF6352 at 16.235 kb on - strand, within GFF6352 at 16.384 kb on + strand, within GFF6352 at 16.558 kb on + strand, within GFF6352 at 16.559 kb on - strand, within GFF6352 at 16.736 kb on - strand, within GFF6352 at 16.736 kb on - strand, within GFF6352 at 16.786 kb on + strand, within GFF6352 at 16.850 kb on - strand, within GFF6352 at 17.042 kb on - strand, within GFF6352 at 17.183 kb on - strand, within GFF6352 at 17.227 kb on - strand, within GFF6352 at 17.452 kb on + strand, within GFF6352 at 17.626 kb on + strand, within GFF6352 at 17.626 kb on + strand, within GFF6352 at 17.626 kb on + strand, within GFF6352 at 17.626 kb on + strand, within GFF6352 at 17.627 kb on - strand, within GFF6352 at 18.030 kb on - strand at 18.396 kb on + strand, within GFF6353 at 18.876 kb on + strand, within GFF6353 at 18.877 kb on - strand, within GFF6353 at 18.877 kb on - strand, within GFF6353
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 13,463 + -0.3 13,463 + -0.8 13,463 + -0.1 13,463 + -0.2 13,463 + +0.6 13,463 + -0.3 13,463 + -0.8 13,464 - -0.7 13,464 - -1.1 13,464 - +0.3 13,464 - -2.1 13,464 - +0.2 13,503 - -0.2 13,797 + GFF6351 0.16 +0.5 13,879 + GFF6351 0.28 +0.1 13,879 + GFF6351 0.28 +0.1 14,025 + GFF6351 0.48 -0.2 14,025 + GFF6351 0.48 -0.3 14,062 - GFF6351 0.53 -0.8 14,103 + GFF6351 0.59 -0.9 14,205 + GFF6351 0.73 +0.3 14,206 - GFF6351 0.73 -0.7 14,206 - GFF6351 0.73 -1.4 14,452 + -0.6 14,452 + -0.5 14,452 + +1.5 14,452 + +0.1 14,453 - +0.2 14,702 - +0.8 14,860 + GFF6352 0.13 -0.4 15,277 + GFF6352 0.24 -0.9 15,277 + GFF6352 0.24 +0.9 15,278 - GFF6352 0.24 +0.0 15,278 - GFF6352 0.24 +0.4 15,673 + GFF6352 0.35 +0.3 15,674 - GFF6352 0.35 -0.6 15,960 + GFF6352 0.43 +2.1 15,960 + GFF6352 0.43 +0.7 15,961 - GFF6352 0.43 +0.3 15,980 - GFF6352 0.43 -0.2 16,216 + GFF6352 0.50 +0.1 16,235 - GFF6352 0.50 -0.0 16,235 - GFF6352 0.50 -0.4 16,235 - GFF6352 0.50 -1.9 16,384 + GFF6352 0.54 -0.4 16,558 + GFF6352 0.59 +0.3 16,559 - GFF6352 0.59 +0.6 16,736 - GFF6352 0.64 -1.1 16,736 - GFF6352 0.64 +0.6 16,786 + GFF6352 0.65 -0.3 16,850 - GFF6352 0.67 +0.5 17,042 - GFF6352 0.72 -1.8 17,183 - GFF6352 0.76 +0.3 17,227 - GFF6352 0.77 -0.9 17,452 + GFF6352 0.83 -0.8 17,626 + GFF6352 0.88 -0.4 17,626 + GFF6352 0.88 -0.6 17,626 + GFF6352 0.88 -0.4 17,626 + GFF6352 0.88 +0.2 17,627 - GFF6352 0.88 +1.3 18,030 - +0.1 18,396 + GFF6353 0.25 +0.4 18,876 + GFF6353 0.60 -0.1 18,877 - GFF6353 0.60 +0.7 18,877 - GFF6353 0.60 -0.1
Or see this region's nucleotide sequence