Strain Fitness in Variovorax sp. SCN45 around GFF604

Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF603 and GFF604 overlap by 17 nucleotidesGFF604 and GFF605 are separated by 28 nucleotidesGFF605 and GFF606 are separated by 34 nucleotides GFF603 - Tricarballylate dehydrogenase, TcuA, at 647,988 to 649,415 GFF603 GFF604 - Tricarballylate utilization protein, TcuB, at 649,399 to 650,619 GFF604 GFF605 - Aldolase related to tricarballylate utilization, at 650,648 to 651,436 GFF605 GFF606 - BUG/TctC family periplasmic protein, at 651,471 to 652,454 GFF606 Position (kb) 649 650 651Strain fitness (log2 ratio) -2 -1 0 1 2at 648.416 kb on - strand, within GFF603at 648.426 kb on + strand, within GFF603at 648.439 kb on + strand, within GFF603at 648.736 kb on - strand, within GFF603at 648.736 kb on - strand, within GFF603at 648.810 kb on + strand, within GFF603at 648.811 kb on - strand, within GFF603at 648.843 kb on + strand, within GFF603at 648.843 kb on + strand, within GFF603at 648.844 kb on - strand, within GFF603at 648.844 kb on - strand, within GFF603at 649.026 kb on + strand, within GFF603at 649.026 kb on + strand, within GFF603at 649.179 kb on + strand, within GFF603at 649.179 kb on + strand, within GFF603at 649.180 kb on - strand, within GFF603at 649.180 kb on - strand, within GFF603at 649.180 kb on - strand, within GFF603at 649.180 kb on - strand, within GFF603at 649.180 kb on - strand, within GFF603at 649.180 kb on - strand, within GFF603at 649.290 kb on + strandat 649.290 kb on + strandat 649.290 kb on + strandat 649.291 kb on - strandat 649.549 kb on + strand, within GFF604at 649.621 kb on + strand, within GFF604at 649.621 kb on + strand, within GFF604at 649.621 kb on + strand, within GFF604at 649.622 kb on - strand, within GFF604at 649.622 kb on - strand, within GFF604at 649.672 kb on + strand, within GFF604at 649.673 kb on - strand, within GFF604at 649.738 kb on + strand, within GFF604at 649.739 kb on - strand, within GFF604at 649.739 kb on - strand, within GFF604at 649.739 kb on - strand, within GFF604at 649.739 kb on - strand, within GFF604at 649.739 kb on - strand, within GFF604at 649.747 kb on + strand, within GFF604at 649.747 kb on + strand, within GFF604at 649.748 kb on - strand, within GFF604at 649.895 kb on - strand, within GFF604at 649.895 kb on - strand, within GFF604at 650.089 kb on + strand, within GFF604at 650.089 kb on + strand, within GFF604at 650.089 kb on + strand, within GFF604at 650.089 kb on + strand, within GFF604at 650.090 kb on - strand, within GFF604at 650.090 kb on - strand, within GFF604at 650.177 kb on - strand, within GFF604at 650.177 kb on - strand, within GFF604at 650.177 kb on - strand, within GFF604at 650.221 kb on + strand, within GFF604at 650.221 kb on + strand, within GFF604at 650.222 kb on - strand, within GFF604at 650.222 kb on - strand, within GFF604at 650.222 kb on - strand, within GFF604at 650.222 kb on - strand, within GFF604at 650.222 kb on - strand, within GFF604at 650.228 kb on - strand, within GFF604at 650.228 kb on - strand, within GFF604at 650.252 kb on - strand, within GFF604at 650.664 kb on + strandat 650.664 kb on + strandat 650.800 kb on - strand, within GFF605at 650.846 kb on + strand, within GFF605at 650.846 kb on + strand, within GFF605at 650.846 kb on + strand, within GFF605at 650.846 kb on + strand, within GFF605at 650.847 kb on - strand, within GFF605at 650.847 kb on - strand, within GFF605at 650.847 kb on - strand, within GFF605at 650.847 kb on - strand, within GFF605at 650.931 kb on - strand, within GFF605at 650.951 kb on + strand, within GFF605at 651.099 kb on - strand, within GFF605at 651.107 kb on + strand, within GFF605at 651.107 kb on + strand, within GFF605at 651.344 kb on + strand, within GFF605at 651.344 kb on + strand, within GFF605at 651.345 kb on - strand, within GFF605at 651.345 kb on - strand, within GFF605at 651.345 kb on - strand, within GFF605at 651.345 kb on - strand, within GFF605at 651.345 kb on - strand, within GFF605at 651.405 kb on + strandat 651.406 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
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648,416 - GFF603 0.30 +0.2
648,426 + GFF603 0.31 +0.9
648,439 + GFF603 0.32 +0.1
648,736 - GFF603 0.52 +1.2
648,736 - GFF603 0.52 -1.3
648,810 + GFF603 0.58 +0.9
648,811 - GFF603 0.58 -0.4
648,843 + GFF603 0.60 +0.9
648,843 + GFF603 0.60 +0.4
648,844 - GFF603 0.60 -0.0
648,844 - GFF603 0.60 +0.2
649,026 + GFF603 0.73 +0.6
649,026 + GFF603 0.73 -0.4
649,179 + GFF603 0.83 -0.5
649,179 + GFF603 0.83 +0.3
649,180 - GFF603 0.83 -0.3
649,180 - GFF603 0.83 +0.3
649,180 - GFF603 0.83 -1.2
649,180 - GFF603 0.83 +0.1
649,180 - GFF603 0.83 -1.0
649,180 - GFF603 0.83 -0.2
649,290 + +0.3
649,290 + -0.1
649,290 + -0.0
649,291 - -1.5
649,549 + GFF604 0.12 -0.3
649,621 + GFF604 0.18 -0.1
649,621 + GFF604 0.18 +1.9
649,621 + GFF604 0.18 -0.0
649,622 - GFF604 0.18 +0.1
649,622 - GFF604 0.18 -2.4
649,672 + GFF604 0.22 -0.4
649,673 - GFF604 0.22 +0.0
649,738 + GFF604 0.28 +0.1
649,739 - GFF604 0.28 -0.5
649,739 - GFF604 0.28 +0.2
649,739 - GFF604 0.28 +0.7
649,739 - GFF604 0.28 +1.0
649,739 - GFF604 0.28 +0.7
649,747 + GFF604 0.29 -0.2
649,747 + GFF604 0.29 +0.3
649,748 - GFF604 0.29 -0.5
649,895 - GFF604 0.41 -0.7
649,895 - GFF604 0.41 -0.5
650,089 + GFF604 0.57 -0.8
650,089 + GFF604 0.57 +0.1
650,089 + GFF604 0.57 +1.1
650,089 + GFF604 0.57 -1.4
650,090 - GFF604 0.57 -0.7
650,090 - GFF604 0.57 +1.0
650,177 - GFF604 0.64 +0.3
650,177 - GFF604 0.64 +0.5
650,177 - GFF604 0.64 +0.0
650,221 + GFF604 0.67 +0.6
650,221 + GFF604 0.67 -0.1
650,222 - GFF604 0.67 -0.5
650,222 - GFF604 0.67 -0.9
650,222 - GFF604 0.67 +0.1
650,222 - GFF604 0.67 -0.8
650,222 - GFF604 0.67 -1.9
650,228 - GFF604 0.68 -1.4
650,228 - GFF604 0.68 -0.3
650,252 - GFF604 0.70 -0.1
650,664 + +0.5
650,664 + -0.7
650,800 - GFF605 0.19 -1.5
650,846 + GFF605 0.25 -1.6
650,846 + GFF605 0.25 -0.8
650,846 + GFF605 0.25 -0.0
650,846 + GFF605 0.25 -1.0
650,847 - GFF605 0.25 +2.2
650,847 - GFF605 0.25 +0.5
650,847 - GFF605 0.25 -0.8
650,847 - GFF605 0.25 -0.6
650,931 - GFF605 0.36 +0.6
650,951 + GFF605 0.38 -0.9
651,099 - GFF605 0.57 +0.5
651,107 + GFF605 0.58 +0.5
651,107 + GFF605 0.58 +0.4
651,344 + GFF605 0.88 +0.9
651,344 + GFF605 0.88 +0.7
651,345 - GFF605 0.88 -0.3
651,345 - GFF605 0.88 +0.3
651,345 - GFF605 0.88 -1.1
651,345 - GFF605 0.88 -0.7
651,345 - GFF605 0.88 +0.2
651,405 + -0.0
651,406 - -0.3

Or see this region's nucleotide sequence