Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5888 and GFF5889 are separated by 534 nucleotides GFF5889 and GFF5890 overlap by 4 nucleotides GFF5890 and GFF5891 are separated by 31 nucleotides
GFF5888 - Translation elongation factor Tu, at 107,144 to 108,337
GFF5888
GFF5889 - Uracil-DNA glycosylase, family 1 (EC 3.2.2.27), at 108,872 to 109,579
GFF5889
GFF5890 - Indole-3-glycerol phosphate synthase (EC 4.1.1.48), at 109,576 to 110,376
GFF5890
GFF5891 - Anthranilate phosphoribosyltransferase (EC 2.4.2.18), at 110,408 to 111,430
GFF5891
Position (kb)
108
109
110 Strain fitness (log2 ratio)
-1
0
1
2 at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.496 kb on - strand at 108.496 kb on - strand at 108.496 kb on - strand at 108.496 kb on - strand at 108.496 kb on - strand at 108.496 kb on - strand at 108.496 kb on - strand at 108.643 kb on - strand at 108.643 kb on - strand at 108.659 kb on - strand at 108.659 kb on - strand at 108.659 kb on - strand at 108.659 kb on - strand at 108.659 kb on - strand at 108.797 kb on - strand at 108.821 kb on - strand at 108.994 kb on + strand, within GFF5889 at 108.994 kb on + strand, within GFF5889 at 108.994 kb on + strand, within GFF5889 at 108.995 kb on - strand, within GFF5889 at 108.995 kb on - strand, within GFF5889 at 108.995 kb on - strand, within GFF5889 at 109.025 kb on - strand, within GFF5889 at 109.025 kb on - strand, within GFF5889 at 109.240 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.257 kb on - strand, within GFF5889 at 109.257 kb on - strand, within GFF5889 at 109.257 kb on - strand, within GFF5889 at 109.257 kb on - strand, within GFF5889 at 109.257 kb on - strand, within GFF5889 at 109.270 kb on + strand, within GFF5889 at 109.270 kb on + strand, within GFF5889 at 109.270 kb on + strand, within GFF5889 at 109.270 kb on + strand, within GFF5889 at 109.270 kb on + strand, within GFF5889 at 109.270 kb on + strand, within GFF5889 at 109.271 kb on - strand, within GFF5889 at 109.271 kb on - strand, within GFF5889 at 109.347 kb on + strand, within GFF5889 at 109.348 kb on - strand, within GFF5889 at 109.393 kb on + strand, within GFF5889 at 109.394 kb on - strand, within GFF5889 at 109.394 kb on - strand, within GFF5889 at 109.394 kb on - strand, within GFF5889 at 110.377 kb on - strand at 110.377 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 108,369 - -1.5 108,369 - -0.7 108,369 - -1.0 108,369 - +0.1 108,369 - +1.3 108,369 - -1.0 108,369 - -1.5 108,369 - -0.0 108,369 - -0.6 108,496 - -1.6 108,496 - -0.2 108,496 - +0.8 108,496 - -0.3 108,496 - -0.6 108,496 - +0.8 108,496 - -0.3 108,643 - +0.5 108,643 - +0.0 108,659 - -0.2 108,659 - -0.5 108,659 - -1.7 108,659 - +0.9 108,659 - -0.4 108,797 - -0.0 108,821 - -0.7 108,994 + GFF5889 0.17 +0.3 108,994 + GFF5889 0.17 -1.5 108,994 + GFF5889 0.17 +1.1 108,995 - GFF5889 0.17 +0.0 108,995 - GFF5889 0.17 -0.6 108,995 - GFF5889 0.17 +0.1 109,025 - GFF5889 0.22 -0.9 109,025 - GFF5889 0.22 -0.3 109,240 + GFF5889 0.52 -1.1 109,256 + GFF5889 0.54 +0.3 109,256 + GFF5889 0.54 +0.3 109,256 + GFF5889 0.54 -0.4 109,256 + GFF5889 0.54 +0.2 109,256 + GFF5889 0.54 -0.4 109,256 + GFF5889 0.54 +1.2 109,256 + GFF5889 0.54 -0.1 109,256 + GFF5889 0.54 +0.1 109,256 + GFF5889 0.54 +0.3 109,256 + GFF5889 0.54 +0.5 109,257 - GFF5889 0.54 -0.4 109,257 - GFF5889 0.54 -0.3 109,257 - GFF5889 0.54 +0.3 109,257 - GFF5889 0.54 +0.1 109,257 - GFF5889 0.54 -0.7 109,270 + GFF5889 0.56 +0.1 109,270 + GFF5889 0.56 +0.4 109,270 + GFF5889 0.56 -0.2 109,270 + GFF5889 0.56 -0.3 109,270 + GFF5889 0.56 -0.6 109,270 + GFF5889 0.56 +0.0 109,271 - GFF5889 0.56 +1.0 109,271 - GFF5889 0.56 +0.4 109,347 + GFF5889 0.67 -0.2 109,348 - GFF5889 0.67 +0.3 109,393 + GFF5889 0.74 -0.6 109,394 - GFF5889 0.74 -0.2 109,394 - GFF5889 0.74 +0.6 109,394 - GFF5889 0.74 +1.1 110,377 - +0.4 110,377 - +2.3
Or see this region's nucleotide sequence