Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5649 and GFF5650 are separated by 128 nucleotides GFF5650 and GFF5651 are separated by 13 nucleotides
GFF5649 - Selenide,water dikinase (EC 2.7.9.3), at 23,792 to 24,859
GFF5649
GFF5650 - Selenocysteine-specific translation elongation factor, at 24,988 to 26,895
GFF5650
GFF5651 - hypothetical protein, at 26,909 to 28,111
GFF5651
Position (kb) 24
25
26
27 Strain fitness (log2 ratio) -2
-1
0
1 at 24.041 kb on + strand, within GFF5649 at 24.042 kb on - strand, within GFF5649 at 24.086 kb on + strand, within GFF5649 at 24.086 kb on + strand, within GFF5649 at 24.086 kb on + strand, within GFF5649 at 24.086 kb on + strand, within GFF5649 at 24.087 kb on - strand, within GFF5649 at 24.087 kb on - strand, within GFF5649 at 24.087 kb on - strand, within GFF5649 at 24.087 kb on - strand, within GFF5649 at 24.254 kb on + strand, within GFF5649 at 24.255 kb on - strand, within GFF5649 at 24.255 kb on - strand, within GFF5649 at 24.255 kb on - strand, within GFF5649 at 24.858 kb on - strand at 25.014 kb on + strand at 25.014 kb on + strand at 25.015 kb on - strand at 25.206 kb on - strand, within GFF5650 at 25.206 kb on - strand, within GFF5650 at 25.206 kb on - strand, within GFF5650 at 25.206 kb on - strand, within GFF5650 at 25.208 kb on + strand, within GFF5650 at 25.208 kb on + strand, within GFF5650 at 25.208 kb on + strand, within GFF5650 at 25.208 kb on + strand, within GFF5650 at 25.209 kb on - strand, within GFF5650 at 25.209 kb on - strand, within GFF5650 at 25.209 kb on - strand, within GFF5650 at 25.209 kb on - strand, within GFF5650 at 25.209 kb on - strand, within GFF5650 at 25.209 kb on - strand, within GFF5650 at 26.602 kb on + strand, within GFF5650 at 26.603 kb on - strand, within GFF5650 at 26.765 kb on + strand at 26.765 kb on + strand at 26.765 kb on + strand at 26.765 kb on + strand at 26.765 kb on + strand at 26.766 kb on - strand at 26.766 kb on - strand at 26.766 kb on - strand at 26.766 kb on - strand at 26.766 kb on - strand at 26.771 kb on + strand at 26.772 kb on - strand at 26.870 kb on - strand at 27.172 kb on - strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.586 kb on - strand, within GFF5651 at 27.586 kb on - strand, within GFF5651 at 27.586 kb on - strand, within GFF5651 at 27.586 kb on - strand, within GFF5651 at 27.586 kb on - strand, within GFF5651
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 24,041 + GFF5649 0.23 +0.2 24,042 - GFF5649 0.23 +0.2 24,086 + GFF5649 0.28 +0.4 24,086 + GFF5649 0.28 -0.8 24,086 + GFF5649 0.28 -0.4 24,086 + GFF5649 0.28 +0.4 24,087 - GFF5649 0.28 -0.2 24,087 - GFF5649 0.28 -0.2 24,087 - GFF5649 0.28 +1.1 24,087 - GFF5649 0.28 -0.1 24,254 + GFF5649 0.43 -0.2 24,255 - GFF5649 0.43 -1.2 24,255 - GFF5649 0.43 +0.4 24,255 - GFF5649 0.43 -0.4 24,858 - -0.3 25,014 + +0.0 25,014 + +0.0 25,015 - -0.9 25,206 - GFF5650 0.11 -0.5 25,206 - GFF5650 0.11 -0.6 25,206 - GFF5650 0.11 -0.4 25,206 - GFF5650 0.11 +0.1 25,208 + GFF5650 0.12 -0.9 25,208 + GFF5650 0.12 -2.4 25,208 + GFF5650 0.12 +0.3 25,208 + GFF5650 0.12 -1.0 25,209 - GFF5650 0.12 -0.3 25,209 - GFF5650 0.12 -1.0 25,209 - GFF5650 0.12 -1.4 25,209 - GFF5650 0.12 -0.2 25,209 - GFF5650 0.12 -0.8 25,209 - GFF5650 0.12 -0.8 26,602 + GFF5650 0.85 -0.1 26,603 - GFF5650 0.85 -0.1 26,765 + +0.1 26,765 + +0.0 26,765 + +0.5 26,765 + -0.5 26,765 + +0.3 26,766 - -0.5 26,766 - -0.5 26,766 - -0.8 26,766 - +0.1 26,766 - -0.0 26,771 + +0.6 26,772 - -0.8 26,870 - -0.4 27,172 - GFF5651 0.22 -0.5 27,585 + GFF5651 0.56 +0.1 27,585 + GFF5651 0.56 -2.6 27,585 + GFF5651 0.56 +0.2 27,585 + GFF5651 0.56 -0.2 27,585 + GFF5651 0.56 -0.5 27,585 + GFF5651 0.56 +0.0 27,585 + GFF5651 0.56 -0.6 27,585 + GFF5651 0.56 -0.4 27,585 + GFF5651 0.56 +0.7 27,586 - GFF5651 0.56 -1.2 27,586 - GFF5651 0.56 +0.5 27,586 - GFF5651 0.56 -0.2 27,586 - GFF5651 0.56 +0.6 27,586 - GFF5651 0.56 -0.2
Or see this region's nucleotide sequence