Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5646 and GFF5647 overlap by 4 nucleotides GFF5647 and GFF5648 are separated by 10 nucleotides GFF5648 and GFF5649 are separated by 74 nucleotides
GFF5646 - ABC transporter, permease protein (cluster 12, methionine/phosphonates) / ABC transporter, permease protein (cluster 12, methionine/phosphonates), at 21,198 to 22,037
GFF5646
GFF5647 - ABC transporter, ATP-binding protein (cluster 12, methionine/phosphonates), at 22,034 to 22,846
GFF5647
GFF5648 - ABC transporter, substrate-binding protein (cluster 12, methionine/phosphonates), at 22,857 to 23,717
GFF5648
GFF5649 - Selenide,water dikinase (EC 2.7.9.3), at 23,792 to 24,859
GFF5649
Position (kb) 22
23
24 Strain fitness (log2 ratio) -2
-1
0
1
2 at 22.119 kb on + strand, within GFF5647 at 22.119 kb on + strand, within GFF5647 at 22.119 kb on + strand, within GFF5647 at 22.120 kb on - strand, within GFF5647 at 22.362 kb on + strand, within GFF5647 at 22.363 kb on - strand, within GFF5647 at 22.363 kb on - strand, within GFF5647 at 22.363 kb on - strand, within GFF5647 at 22.363 kb on - strand, within GFF5647 at 22.903 kb on + strand at 22.903 kb on + strand at 22.903 kb on + strand at 22.903 kb on + strand at 22.903 kb on + strand at 22.903 kb on + strand at 22.904 kb on - strand at 22.904 kb on - strand at 22.904 kb on - strand at 23.053 kb on + strand, within GFF5648 at 23.053 kb on + strand, within GFF5648 at 23.053 kb on + strand, within GFF5648 at 23.054 kb on - strand, within GFF5648 at 23.054 kb on - strand, within GFF5648 at 23.059 kb on + strand, within GFF5648 at 23.059 kb on + strand, within GFF5648 at 23.059 kb on + strand, within GFF5648 at 23.059 kb on + strand, within GFF5648 at 23.060 kb on - strand, within GFF5648 at 23.060 kb on - strand, within GFF5648 at 23.060 kb on - strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.063 kb on - strand, within GFF5648 at 23.063 kb on - strand, within GFF5648 at 23.063 kb on - strand, within GFF5648 at 23.063 kb on - strand, within GFF5648 at 23.063 kb on - strand, within GFF5648 at 23.264 kb on - strand, within GFF5648 at 23.264 kb on - strand, within GFF5648 at 23.566 kb on + strand, within GFF5648 at 23.566 kb on + strand, within GFF5648 at 23.743 kb on + strand at 23.761 kb on + strand at 23.761 kb on + strand at 23.761 kb on + strand at 23.762 kb on - strand at 23.762 kb on - strand at 23.762 kb on - strand at 23.972 kb on + strand, within GFF5649 at 23.972 kb on + strand, within GFF5649 at 23.973 kb on - strand, within GFF5649 at 24.041 kb on + strand, within GFF5649 at 24.042 kb on - strand, within GFF5649 at 24.086 kb on + strand, within GFF5649 at 24.086 kb on + strand, within GFF5649 at 24.086 kb on + strand, within GFF5649 at 24.086 kb on + strand, within GFF5649 at 24.087 kb on - strand, within GFF5649 at 24.087 kb on - strand, within GFF5649 at 24.087 kb on - strand, within GFF5649 at 24.087 kb on - strand, within GFF5649 at 24.254 kb on + strand, within GFF5649 at 24.255 kb on - strand, within GFF5649 at 24.255 kb on - strand, within GFF5649 at 24.255 kb on - strand, within GFF5649
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 22,119 + GFF5647 0.10 -0.2 22,119 + GFF5647 0.10 -1.0 22,119 + GFF5647 0.10 -0.8 22,120 - GFF5647 0.11 -0.6 22,362 + GFF5647 0.40 +0.3 22,363 - GFF5647 0.40 -0.2 22,363 - GFF5647 0.40 -2.3 22,363 - GFF5647 0.40 -0.8 22,363 - GFF5647 0.40 -0.2 22,903 + -0.7 22,903 + +0.4 22,903 + +0.4 22,903 + +0.2 22,903 + -0.2 22,903 + -1.5 22,904 - +0.3 22,904 - -0.0 22,904 - +2.0 23,053 + GFF5648 0.23 +1.8 23,053 + GFF5648 0.23 -0.8 23,053 + GFF5648 0.23 -0.2 23,054 - GFF5648 0.23 -1.2 23,054 - GFF5648 0.23 +0.5 23,059 + GFF5648 0.23 +1.4 23,059 + GFF5648 0.23 -0.2 23,059 + GFF5648 0.23 +0.9 23,059 + GFF5648 0.23 +0.5 23,060 - GFF5648 0.24 +0.6 23,060 - GFF5648 0.24 +0.0 23,060 - GFF5648 0.24 +0.2 23,062 + GFF5648 0.24 -0.5 23,062 + GFF5648 0.24 +1.8 23,062 + GFF5648 0.24 -0.6 23,062 + GFF5648 0.24 +0.2 23,062 + GFF5648 0.24 -0.3 23,062 + GFF5648 0.24 +0.6 23,062 + GFF5648 0.24 +0.2 23,062 + GFF5648 0.24 +0.5 23,063 - GFF5648 0.24 -0.6 23,063 - GFF5648 0.24 +0.4 23,063 - GFF5648 0.24 +0.2 23,063 - GFF5648 0.24 +1.8 23,063 - GFF5648 0.24 +0.2 23,264 - GFF5648 0.47 +0.2 23,264 - GFF5648 0.47 -1.4 23,566 + GFF5648 0.82 +0.2 23,566 + GFF5648 0.82 -0.2 23,743 + +0.8 23,761 + -0.5 23,761 + -0.6 23,761 + -0.4 23,762 - -0.7 23,762 - -0.4 23,762 - -0.2 23,972 + GFF5649 0.17 -1.0 23,972 + GFF5649 0.17 +0.2 23,973 - GFF5649 0.17 -0.4 24,041 + GFF5649 0.23 +0.2 24,042 - GFF5649 0.23 +0.2 24,086 + GFF5649 0.28 +0.4 24,086 + GFF5649 0.28 -0.8 24,086 + GFF5649 0.28 -0.4 24,086 + GFF5649 0.28 +0.4 24,087 - GFF5649 0.28 -0.2 24,087 - GFF5649 0.28 -0.2 24,087 - GFF5649 0.28 +1.1 24,087 - GFF5649 0.28 -0.1 24,254 + GFF5649 0.43 -0.2 24,255 - GFF5649 0.43 -1.2 24,255 - GFF5649 0.43 +0.4 24,255 - GFF5649 0.43 -0.4
Or see this region's nucleotide sequence