Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF508 and GFF509 are separated by 16 nucleotides GFF509 and GFF510 overlap by 4 nucleotides GFF510 and GFF511 overlap by 4 nucleotides GFF511 and GFF512 overlap by 4 nucleotides GFF512 and GFF513 are separated by 44 nucleotides
GFF508 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 549,582 to 550,649
GFF508
GFF509 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 550,666 to 551,646
GFF509
GFF510 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 551,643 to 552,371
GFF510
GFF511 - no description, at 552,368 to 552,514
GFF511
GFF512 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 552,511 to 553,278
GFF512
GFF513 - Chitinase (EC 3.2.1.14), at 553,323 to 554,780
GFF513
Position (kb) 551
552
553 Strain fitness (log2 ratio) -2
-1
0
1
2 at 550.685 kb on + strand at 550.686 kb on - strand at 550.772 kb on + strand, within GFF509 at 550.772 kb on + strand, within GFF509 at 550.772 kb on + strand, within GFF509 at 550.772 kb on + strand, within GFF509 at 550.772 kb on + strand, within GFF509 at 550.772 kb on + strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 551.647 kb on - strand at 551.647 kb on - strand at 551.647 kb on - strand at 552.118 kb on + strand, within GFF510 at 552.118 kb on + strand, within GFF510 at 552.118 kb on + strand, within GFF510 at 552.118 kb on + strand, within GFF510 at 552.119 kb on - strand, within GFF510 at 552.119 kb on - strand, within GFF510 at 552.119 kb on - strand, within GFF510 at 552.119 kb on - strand, within GFF510 at 552.316 kb on + strand at 552.317 kb on - strand at 552.375 kb on + strand at 552.375 kb on + strand at 552.376 kb on - strand at 552.542 kb on + strand at 552.542 kb on + strand at 552.542 kb on + strand at 552.543 kb on - strand at 552.543 kb on - strand at 553.151 kb on + strand, within GFF512 at 553.151 kb on + strand, within GFF512 at 553.151 kb on + strand, within GFF512 at 553.151 kb on + strand, within GFF512 at 553.152 kb on - strand, within GFF512 at 553.152 kb on - strand, within GFF512 at 553.152 kb on - strand, within GFF512 at 553.152 kb on - strand, within GFF512 at 553.152 kb on - strand, within GFF512 at 553.152 kb on - strand, within GFF512 at 553.342 kb on + strand at 553.342 kb on + strand at 553.342 kb on + strand at 553.342 kb on + strand at 553.343 kb on - strand at 553.343 kb on - strand at 553.343 kb on - strand at 553.345 kb on + strand at 553.345 kb on + strand at 553.345 kb on + strand at 553.345 kb on + strand at 553.346 kb on - strand at 553.346 kb on - strand at 553.346 kb on - strand at 553.346 kb on - strand at 553.346 kb on - strand at 553.346 kb on - strand at 553.346 kb on - strand at 553.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 550,685 + +1.9 550,686 - +1.2 550,772 + GFF509 0.11 +0.7 550,772 + GFF509 0.11 +0.2 550,772 + GFF509 0.11 +2.3 550,772 + GFF509 0.11 -1.4 550,772 + GFF509 0.11 -0.1 550,772 + GFF509 0.11 -0.4 550,773 - GFF509 0.11 +0.3 550,773 - GFF509 0.11 +1.5 550,773 - GFF509 0.11 -1.5 550,773 - GFF509 0.11 +0.2 550,773 - GFF509 0.11 -0.2 550,773 - GFF509 0.11 +0.1 550,773 - GFF509 0.11 +0.3 550,773 - GFF509 0.11 -0.4 551,647 - +0.1 551,647 - -1.2 551,647 - -0.5 552,118 + GFF510 0.65 -0.7 552,118 + GFF510 0.65 -1.9 552,118 + GFF510 0.65 -0.3 552,118 + GFF510 0.65 +0.5 552,119 - GFF510 0.65 +0.0 552,119 - GFF510 0.65 -0.3 552,119 - GFF510 0.65 -1.1 552,119 - GFF510 0.65 -1.5 552,316 + -0.2 552,317 - +0.4 552,375 + -1.1 552,375 + -0.9 552,376 - -0.1 552,542 + +0.3 552,542 + +0.5 552,542 + -0.3 552,543 - -1.6 552,543 - -0.2 553,151 + GFF512 0.83 -0.1 553,151 + GFF512 0.83 -0.5 553,151 + GFF512 0.83 -0.4 553,151 + GFF512 0.83 +0.5 553,152 - GFF512 0.83 +0.3 553,152 - GFF512 0.83 -0.3 553,152 - GFF512 0.83 -0.5 553,152 - GFF512 0.83 -1.1 553,152 - GFF512 0.83 +0.8 553,152 - GFF512 0.83 -0.2 553,342 + -0.4 553,342 + -0.4 553,342 + -1.0 553,342 + -0.9 553,343 - -0.9 553,343 - -0.4 553,343 - -0.8 553,345 + -0.4 553,345 + -0.7 553,345 + -0.1 553,345 + -0.4 553,346 - +0.6 553,346 - -2.3 553,346 - -0.4 553,346 - -0.2 553,346 - -0.9 553,346 - -0.5 553,346 - -0.7 553,346 - -0.2
Or see this region's nucleotide sequence