Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF482 and GFF483 are separated by 56 nucleotides GFF483 and GFF484 are separated by 1 nucleotides GFF484 and GFF485 are separated by 18 nucleotides
GFF482 - Glycine betaine/L-proline transport substrate-binding protein ProX (TC 3.A.1.12.1), at 520,966 to 521,985
GFF482
GFF483 - Fumarate hydratase class I, aerobic (EC 4.2.1.2), at 522,042 to 523,595
GFF483
GFF484 - Glutamate racemase (EC 5.1.1.3), at 523,597 to 524,397
GFF484
GFF485 - hypothetical protein, at 524,416 to 525,201
GFF485
Position (kb) 522
523
524 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 521.155 kb on + strand, within GFF482 at 521.155 kb on + strand, within GFF482 at 521.156 kb on - strand, within GFF482 at 521.156 kb on - strand, within GFF482 at 521.156 kb on - strand, within GFF482 at 521.156 kb on - strand, within GFF482 at 521.156 kb on - strand, within GFF482 at 521.266 kb on + strand, within GFF482 at 521.266 kb on + strand, within GFF482 at 521.267 kb on - strand, within GFF482 at 521.267 kb on - strand, within GFF482 at 521.267 kb on - strand, within GFF482 at 521.311 kb on + strand, within GFF482 at 521.311 kb on + strand, within GFF482 at 521.311 kb on + strand, within GFF482 at 521.311 kb on + strand, within GFF482 at 521.312 kb on - strand, within GFF482 at 521.312 kb on - strand, within GFF482 at 521.312 kb on - strand, within GFF482 at 521.312 kb on - strand, within GFF482 at 521.335 kb on + strand, within GFF482 at 521.335 kb on + strand, within GFF482 at 521.336 kb on - strand, within GFF482 at 521.336 kb on - strand, within GFF482 at 521.366 kb on - strand, within GFF482 at 521.366 kb on - strand, within GFF482 at 521.506 kb on + strand, within GFF482 at 521.507 kb on - strand, within GFF482 at 521.545 kb on + strand, within GFF482 at 521.545 kb on + strand, within GFF482 at 521.545 kb on + strand, within GFF482 at 521.545 kb on + strand, within GFF482 at 521.546 kb on - strand, within GFF482 at 521.546 kb on - strand, within GFF482 at 521.546 kb on - strand, within GFF482 at 521.602 kb on + strand, within GFF482 at 521.602 kb on + strand, within GFF482 at 521.602 kb on + strand, within GFF482 at 521.602 kb on + strand, within GFF482 at 521.602 kb on + strand, within GFF482 at 521.602 kb on + strand, within GFF482 at 521.602 kb on + strand, within GFF482 at 521.602 kb on + strand, within GFF482 at 521.602 kb on + strand, within GFF482 at 521.603 kb on - strand, within GFF482 at 521.603 kb on - strand, within GFF482 at 521.603 kb on - strand, within GFF482 at 521.603 kb on - strand, within GFF482 at 521.603 kb on - strand, within GFF482 at 521.603 kb on - strand, within GFF482 at 521.603 kb on - strand, within GFF482 at 521.603 kb on - strand, within GFF482 at 521.603 kb on - strand, within GFF482 at 521.605 kb on + strand, within GFF482 at 521.605 kb on + strand, within GFF482 at 521.605 kb on + strand, within GFF482 at 521.605 kb on + strand, within GFF482 at 521.605 kb on + strand, within GFF482 at 521.605 kb on + strand, within GFF482 at 521.605 kb on + strand, within GFF482 at 521.605 kb on + strand, within GFF482 at 521.606 kb on - strand, within GFF482 at 521.606 kb on - strand, within GFF482 at 521.606 kb on - strand, within GFF482 at 521.606 kb on - strand, within GFF482 at 521.606 kb on - strand, within GFF482 at 521.606 kb on - strand, within GFF482 at 521.620 kb on + strand, within GFF482 at 521.621 kb on - strand, within GFF482 at 521.621 kb on - strand, within GFF482 at 521.737 kb on + strand, within GFF482 at 521.737 kb on + strand, within GFF482 at 521.737 kb on + strand, within GFF482 at 521.738 kb on - strand, within GFF482 at 521.738 kb on - strand, within GFF482 at 521.738 kb on - strand, within GFF482 at 522.000 kb on + strand at 522.000 kb on + strand at 522.099 kb on + strand at 522.099 kb on + strand at 522.099 kb on + strand at 522.099 kb on + strand at 522.099 kb on + strand at 522.099 kb on + strand at 522.099 kb on + strand at 522.099 kb on + strand at 522.099 kb on + strand at 522.099 kb on + strand at 522.099 kb on + strand at 522.099 kb on + strand at 522.099 kb on + strand at 522.099 kb on + strand at 522.100 kb on - strand at 522.100 kb on - strand at 522.100 kb on - strand at 522.100 kb on - strand at 522.100 kb on - strand at 522.100 kb on - strand at 522.100 kb on - strand at 522.100 kb on - strand at 522.100 kb on - strand at 522.100 kb on - strand at 522.100 kb on - strand at 522.108 kb on + strand at 522.108 kb on + strand at 522.108 kb on + strand at 522.108 kb on + strand at 522.108 kb on + strand at 522.108 kb on + strand at 522.108 kb on + strand at 522.108 kb on + strand at 522.108 kb on + strand at 522.108 kb on + strand at 522.108 kb on + strand at 522.109 kb on - strand at 522.109 kb on - strand at 522.109 kb on - strand at 522.109 kb on - strand at 522.109 kb on - strand at 522.109 kb on - strand at 522.109 kb on - strand at 522.109 kb on - strand at 522.109 kb on - strand at 522.109 kb on - strand at 522.111 kb on + strand at 522.111 kb on + strand at 522.111 kb on + strand at 522.111 kb on + strand at 522.111 kb on + strand at 522.111 kb on + strand at 522.111 kb on + strand at 522.112 kb on - strand at 522.112 kb on - strand at 522.112 kb on - strand at 522.112 kb on - strand at 522.126 kb on + strand at 522.126 kb on + strand at 522.126 kb on + strand at 522.126 kb on + strand at 522.126 kb on + strand at 522.126 kb on + strand at 522.126 kb on + strand at 522.127 kb on - strand at 522.127 kb on - strand at 522.127 kb on - strand at 522.127 kb on - strand at 522.127 kb on - strand at 522.127 kb on - strand at 522.127 kb on - strand at 522.150 kb on + strand at 522.150 kb on + strand at 522.351 kb on + strand, within GFF483 at 522.351 kb on + strand, within GFF483 at 522.351 kb on + strand, within GFF483 at 522.351 kb on + strand, within GFF483 at 522.352 kb on - strand, within GFF483 at 522.352 kb on - strand, within GFF483 at 522.759 kb on + strand, within GFF483 at 522.759 kb on + strand, within GFF483 at 522.759 kb on + strand, within GFF483 at 522.759 kb on + strand, within GFF483 at 522.760 kb on - strand, within GFF483 at 522.760 kb on - strand, within GFF483 at 522.760 kb on - strand, within GFF483 at 522.760 kb on - strand, within GFF483 at 522.760 kb on - strand, within GFF483 at 522.840 kb on + strand, within GFF483 at 522.840 kb on + strand, within GFF483 at 522.840 kb on + strand, within GFF483 at 522.840 kb on + strand, within GFF483 at 522.840 kb on + strand, within GFF483 at 522.840 kb on + strand, within GFF483 at 522.840 kb on + strand, within GFF483 at 522.840 kb on + strand, within GFF483 at 522.840 kb on + strand, within GFF483 at 522.841 kb on - strand, within GFF483 at 522.841 kb on - strand, within GFF483 at 522.841 kb on - strand, within GFF483 at 522.841 kb on - strand, within GFF483 at 522.841 kb on - strand, within GFF483 at 522.841 kb on - strand, within GFF483 at 522.841 kb on - strand, within GFF483 at 522.841 kb on - strand, within GFF483 at 522.841 kb on - strand, within GFF483 at 522.841 kb on - strand, within GFF483 at 522.936 kb on + strand, within GFF483 at 522.936 kb on + strand, within GFF483 at 522.937 kb on - strand, within GFF483 at 522.937 kb on - strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.176 kb on + strand, within GFF483 at 523.177 kb on - strand, within GFF483 at 523.177 kb on - strand, within GFF483 at 523.177 kb on - strand, within GFF483 at 523.177 kb on - strand, within GFF483 at 523.177 kb on - strand, within GFF483 at 523.177 kb on - strand, within GFF483 at 523.177 kb on - strand, within GFF483 at 523.177 kb on - strand, within GFF483 at 523.177 kb on - strand, within GFF483 at 523.177 kb on - strand, within GFF483 at 523.177 kb on - strand, within GFF483 at 523.177 kb on - strand, within GFF483 at 523.179 kb on + strand, within GFF483 at 523.179 kb on + strand, within GFF483 at 523.179 kb on + strand, within GFF483 at 523.179 kb on + strand, within GFF483 at 523.179 kb on + strand, within GFF483 at 523.179 kb on + strand, within GFF483 at 523.179 kb on + strand, within GFF483 at 523.179 kb on + strand, within GFF483 at 523.179 kb on + strand, within GFF483 at 523.180 kb on - strand, within GFF483 at 523.180 kb on - strand, within GFF483 at 523.180 kb on - strand, within GFF483 at 523.180 kb on - strand, within GFF483 at 523.180 kb on - strand, within GFF483 at 523.180 kb on - strand, within GFF483 at 523.180 kb on - strand, within GFF483 at 523.359 kb on + strand, within GFF483 at 523.359 kb on + strand, within GFF483 at 523.359 kb on + strand, within GFF483 at 523.359 kb on + strand, within GFF483 at 523.359 kb on + strand, within GFF483 at 523.360 kb on - strand, within GFF483 at 523.360 kb on - strand, within GFF483 at 523.360 kb on - strand, within GFF483 at 523.360 kb on - strand, within GFF483 at 523.360 kb on - strand, within GFF483 at 523.360 kb on - strand, within GFF483 at 523.386 kb on + strand, within GFF483 at 523.387 kb on - strand, within GFF483 at 523.387 kb on - strand, within GFF483 at 523.387 kb on - strand, within GFF483 at 523.387 kb on - strand, within GFF483 at 523.455 kb on + strand at 523.455 kb on + strand at 523.455 kb on + strand at 523.456 kb on - strand at 523.456 kb on - strand at 523.456 kb on - strand at 523.456 kb on - strand at 523.607 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 521,155 + GFF482 0.19 +0.0 521,155 + GFF482 0.19 -0.6 521,156 - GFF482 0.19 -0.5 521,156 - GFF482 0.19 -1.5 521,156 - GFF482 0.19 -0.4 521,156 - GFF482 0.19 -0.5 521,156 - GFF482 0.19 +0.0 521,266 + GFF482 0.29 -0.7 521,266 + GFF482 0.29 +2.5 521,267 - GFF482 0.30 -0.5 521,267 - GFF482 0.30 -0.2 521,267 - GFF482 0.30 +2.0 521,311 + GFF482 0.34 -0.1 521,311 + GFF482 0.34 -0.7 521,311 + GFF482 0.34 +0.3 521,311 + GFF482 0.34 +0.9 521,312 - GFF482 0.34 -0.3 521,312 - GFF482 0.34 -0.6 521,312 - GFF482 0.34 -0.1 521,312 - GFF482 0.34 +0.1 521,335 + GFF482 0.36 +0.0 521,335 + GFF482 0.36 -0.4 521,336 - GFF482 0.36 -0.5 521,336 - GFF482 0.36 -0.1 521,366 - GFF482 0.39 -0.4 521,366 - GFF482 0.39 -0.8 521,506 + GFF482 0.53 +1.5 521,507 - GFF482 0.53 +0.2 521,545 + GFF482 0.57 -0.9 521,545 + GFF482 0.57 +0.3 521,545 + GFF482 0.57 -1.5 521,545 + GFF482 0.57 +1.1 521,546 - GFF482 0.57 -0.4 521,546 - GFF482 0.57 -0.1 521,546 - GFF482 0.57 +0.1 521,602 + GFF482 0.62 -0.4 521,602 + GFF482 0.62 -0.4 521,602 + GFF482 0.62 -1.0 521,602 + GFF482 0.62 +0.6 521,602 + GFF482 0.62 -0.2 521,602 + GFF482 0.62 +0.8 521,602 + GFF482 0.62 -0.1 521,602 + GFF482 0.62 +2.0 521,602 + GFF482 0.62 +0.1 521,603 - GFF482 0.62 +1.1 521,603 - GFF482 0.62 -0.2 521,603 - GFF482 0.62 +0.5 521,603 - GFF482 0.62 -0.7 521,603 - GFF482 0.62 -0.7 521,603 - GFF482 0.62 -0.6 521,603 - GFF482 0.62 -1.1 521,603 - GFF482 0.62 +0.7 521,603 - GFF482 0.62 -0.1 521,605 + GFF482 0.63 +0.1 521,605 + GFF482 0.63 +0.2 521,605 + GFF482 0.63 -1.2 521,605 + GFF482 0.63 -0.6 521,605 + GFF482 0.63 -0.5 521,605 + GFF482 0.63 -1.5 521,605 + GFF482 0.63 -0.7 521,605 + GFF482 0.63 -0.7 521,606 - GFF482 0.63 +0.0 521,606 - GFF482 0.63 -0.8 521,606 - GFF482 0.63 +0.0 521,606 - GFF482 0.63 +1.2 521,606 - GFF482 0.63 -1.1 521,606 - GFF482 0.63 -1.5 521,620 + GFF482 0.64 +0.2 521,621 - GFF482 0.64 -0.1 521,621 - GFF482 0.64 -2.6 521,737 + GFF482 0.76 -0.5 521,737 + GFF482 0.76 -1.9 521,737 + GFF482 0.76 +0.5 521,738 - GFF482 0.76 +0.5 521,738 - GFF482 0.76 +0.9 521,738 - GFF482 0.76 -0.1 522,000 + +0.3 522,000 + +0.9 522,099 + -2.9 522,099 + -0.4 522,099 + -0.2 522,099 + +0.5 522,099 + +0.1 522,099 + -0.1 522,099 + +0.5 522,099 + +0.5 522,099 + -0.1 522,099 + +0.4 522,099 + +0.1 522,099 + -1.5 522,099 + -0.1 522,099 + -0.5 522,100 - +1.8 522,100 - -0.5 522,100 - -0.6 522,100 - +0.5 522,100 - -2.0 522,100 - +0.2 522,100 - -1.1 522,100 - -0.5 522,100 - +0.2 522,100 - +0.9 522,100 - +0.5 522,108 + +1.0 522,108 + +0.7 522,108 + +0.5 522,108 + -0.2 522,108 + -0.5 522,108 + +0.3 522,108 + -0.2 522,108 + -0.7 522,108 + -0.5 522,108 + +0.3 522,108 + +0.3 522,109 - +0.2 522,109 - +0.1 522,109 - +0.5 522,109 - +0.2 522,109 - -2.1 522,109 - -0.1 522,109 - +0.4 522,109 - +1.0 522,109 - -0.4 522,109 - +0.3 522,111 + -0.5 522,111 + +0.1 522,111 + -1.9 522,111 + -0.8 522,111 + -0.5 522,111 + +0.1 522,111 + -0.1 522,112 - -0.3 522,112 - +0.0 522,112 - +0.2 522,112 - -0.1 522,126 + -0.2 522,126 + +1.0 522,126 + -0.5 522,126 + -0.9 522,126 + +1.7 522,126 + -0.8 522,126 + -0.5 522,127 - +0.3 522,127 - +1.0 522,127 - -1.0 522,127 - -0.5 522,127 - -1.0 522,127 - +0.3 522,127 - -2.0 522,150 + -0.4 522,150 + +1.5 522,351 + GFF483 0.20 +0.2 522,351 + GFF483 0.20 +0.7 522,351 + GFF483 0.20 +0.7 522,351 + GFF483 0.20 +0.9 522,352 - GFF483 0.20 -0.5 522,352 - GFF483 0.20 -1.4 522,759 + GFF483 0.46 +0.5 522,759 + GFF483 0.46 +0.9 522,759 + GFF483 0.46 -1.1 522,759 + GFF483 0.46 +0.1 522,760 - GFF483 0.46 -0.8 522,760 - GFF483 0.46 +0.0 522,760 - GFF483 0.46 +1.6 522,760 - GFF483 0.46 +0.7 522,760 - GFF483 0.46 +0.5 522,840 + GFF483 0.51 -0.1 522,840 + GFF483 0.51 -0.4 522,840 + GFF483 0.51 +0.0 522,840 + GFF483 0.51 -0.3 522,840 + GFF483 0.51 -0.1 522,840 + GFF483 0.51 +0.2 522,840 + GFF483 0.51 +0.0 522,840 + GFF483 0.51 -0.1 522,840 + GFF483 0.51 +0.5 522,841 - GFF483 0.51 -0.6 522,841 - GFF483 0.51 -0.8 522,841 - GFF483 0.51 +0.0 522,841 - GFF483 0.51 -2.2 522,841 - GFF483 0.51 -2.5 522,841 - GFF483 0.51 +0.5 522,841 - GFF483 0.51 +1.5 522,841 - GFF483 0.51 +0.6 522,841 - GFF483 0.51 -2.2 522,841 - GFF483 0.51 -0.9 522,936 + GFF483 0.58 +0.1 522,936 + GFF483 0.58 -0.3 522,937 - GFF483 0.58 +0.1 522,937 - GFF483 0.58 +1.2 523,176 + GFF483 0.73 +0.4 523,176 + GFF483 0.73 -0.7 523,176 + GFF483 0.73 +1.0 523,176 + GFF483 0.73 -0.0 523,176 + GFF483 0.73 -1.0 523,176 + GFF483 0.73 -0.0 523,176 + GFF483 0.73 +1.8 523,176 + GFF483 0.73 +2.1 523,176 + GFF483 0.73 -0.2 523,176 + GFF483 0.73 +0.0 523,176 + GFF483 0.73 +1.5 523,176 + GFF483 0.73 -0.5 523,176 + GFF483 0.73 +0.2 523,176 + GFF483 0.73 -0.1 523,176 + GFF483 0.73 +0.9 523,176 + GFF483 0.73 -0.6 523,176 + GFF483 0.73 -0.7 523,176 + GFF483 0.73 -0.6 523,177 - GFF483 0.73 +0.5 523,177 - GFF483 0.73 -0.4 523,177 - GFF483 0.73 +2.0 523,177 - GFF483 0.73 +0.6 523,177 - GFF483 0.73 -2.2 523,177 - GFF483 0.73 -0.4 523,177 - GFF483 0.73 +0.5 523,177 - GFF483 0.73 +0.2 523,177 - GFF483 0.73 +0.6 523,177 - GFF483 0.73 -0.0 523,177 - GFF483 0.73 -0.7 523,177 - GFF483 0.73 +0.9 523,179 + GFF483 0.73 -1.5 523,179 + GFF483 0.73 -1.5 523,179 + GFF483 0.73 +0.1 523,179 + GFF483 0.73 +1.0 523,179 + GFF483 0.73 +0.8 523,179 + GFF483 0.73 -0.4 523,179 + GFF483 0.73 +1.5 523,179 + GFF483 0.73 +0.4 523,179 + GFF483 0.73 +0.3 523,180 - GFF483 0.73 +0.9 523,180 - GFF483 0.73 -1.4 523,180 - GFF483 0.73 -0.3 523,180 - GFF483 0.73 -0.5 523,180 - GFF483 0.73 +0.3 523,180 - GFF483 0.73 -0.5 523,180 - GFF483 0.73 -0.9 523,359 + GFF483 0.85 -0.5 523,359 + GFF483 0.85 -0.3 523,359 + GFF483 0.85 +0.2 523,359 + GFF483 0.85 -0.2 523,359 + GFF483 0.85 +1.0 523,360 - GFF483 0.85 +0.1 523,360 - GFF483 0.85 +0.6 523,360 - GFF483 0.85 -0.2 523,360 - GFF483 0.85 +1.1 523,360 - GFF483 0.85 -1.8 523,360 - GFF483 0.85 -0.4 523,386 + GFF483 0.86 -0.2 523,387 - GFF483 0.87 -1.2 523,387 - GFF483 0.87 -0.9 523,387 - GFF483 0.87 -0.1 523,387 - GFF483 0.87 -0.1 523,455 + -1.3 523,455 + +0.5 523,455 + -0.1 523,456 - -0.7 523,456 - +2.5 523,456 - -0.5 523,456 - -1.4 523,607 + -0.6
Or see this region's nucleotide sequence