Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4320 and GFF4321 are separated by 85 nucleotides GFF4321 and GFF4322 are separated by 159 nucleotides
GFF4320 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 241,403 to 242,620
GFF4320
GFF4321 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 242,706 to 244,262
GFF4321
GFF4322 - Acyl-CoA dehydrogenase, at 244,422 to 245,582
GFF4322
Position (kb) 242
243
244
245 Strain fitness (log2 ratio) -2
-1
0
1 at 242.895 kb on + strand, within GFF4321 at 243.084 kb on + strand, within GFF4321 at 243.084 kb on + strand, within GFF4321 at 243.084 kb on + strand, within GFF4321 at 243.084 kb on + strand, within GFF4321 at 243.084 kb on + strand, within GFF4321 at 243.084 kb on + strand, within GFF4321 at 243.123 kb on + strand, within GFF4321 at 243.186 kb on + strand, within GFF4321 at 243.187 kb on - strand, within GFF4321 at 243.198 kb on + strand, within GFF4321 at 243.306 kb on - strand, within GFF4321 at 243.510 kb on + strand, within GFF4321 at 243.510 kb on + strand, within GFF4321 at 243.511 kb on - strand, within GFF4321 at 243.618 kb on + strand, within GFF4321 at 243.915 kb on + strand, within GFF4321 at 243.916 kb on - strand, within GFF4321 at 243.966 kb on + strand, within GFF4321 at 243.967 kb on - strand, within GFF4321 at 244.144 kb on - strand at 244.400 kb on - strand at 244.854 kb on + strand, within GFF4322 at 245.202 kb on + strand, within GFF4322
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 242,895 + GFF4321 0.12 +0.4 243,084 + GFF4321 0.24 +0.8 243,084 + GFF4321 0.24 -0.3 243,084 + GFF4321 0.24 +1.4 243,084 + GFF4321 0.24 -0.6 243,084 + GFF4321 0.24 +0.4 243,084 + GFF4321 0.24 -0.1 243,123 + GFF4321 0.27 -0.2 243,186 + GFF4321 0.31 -0.2 243,187 - GFF4321 0.31 -0.8 243,198 + GFF4321 0.32 +0.1 243,306 - GFF4321 0.39 -0.5 243,510 + GFF4321 0.52 +1.0 243,510 + GFF4321 0.52 -0.9 243,511 - GFF4321 0.52 +0.6 243,618 + GFF4321 0.59 -1.3 243,915 + GFF4321 0.78 +0.4 243,916 - GFF4321 0.78 +0.1 243,966 + GFF4321 0.81 -1.8 243,967 - GFF4321 0.81 -0.5 244,144 - +0.8 244,400 - -1.3 244,854 + GFF4322 0.37 -0.7 245,202 + GFF4322 0.67 -0.8
Or see this region's nucleotide sequence