Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3973 and GFF3974 overlap by 4 nucleotides GFF3974 and GFF3975 are separated by 16 nucleotides GFF3975 and GFF3976 overlap by 4 nucleotides
GFF3973 - FIG01198345: hypothetical protein, at 176,145 to 177,320
GFF3973
GFF3974 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 177,317 to 178,570
GFF3974
GFF3975 - Metal-dependent hydrolase, at 178,587 to 179,399
GFF3975
GFF3976 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 179,396 to 180,130
GFF3976
Position (kb) 177
178
179 Strain fitness (log2 ratio) -2
-1
0
1 at 176.565 kb on + strand, within GFF3973 at 176.566 kb on - strand, within GFF3973 at 176.613 kb on + strand, within GFF3973 at 176.613 kb on + strand, within GFF3973 at 176.614 kb on - strand, within GFF3973 at 176.614 kb on - strand, within GFF3973 at 176.614 kb on - strand, within GFF3973 at 176.626 kb on - strand, within GFF3973 at 176.730 kb on + strand, within GFF3973 at 176.731 kb on - strand, within GFF3973 at 176.731 kb on - strand, within GFF3973 at 176.947 kb on - strand, within GFF3973 at 176.994 kb on + strand, within GFF3973 at 176.994 kb on + strand, within GFF3973 at 176.994 kb on + strand, within GFF3973 at 176.995 kb on - strand, within GFF3973 at 177.270 kb on + strand at 177.270 kb on + strand at 177.271 kb on - strand at 177.333 kb on + strand at 177.333 kb on + strand at 177.334 kb on - strand at 178.145 kb on + strand, within GFF3974 at 178.145 kb on + strand, within GFF3974 at 178.146 kb on - strand, within GFF3974 at 178.146 kb on - strand, within GFF3974 at 178.485 kb on - strand at 178.968 kb on + strand, within GFF3975 at 178.968 kb on + strand, within GFF3975 at 179.212 kb on - strand, within GFF3975 at 179.212 kb on - strand, within GFF3975 at 179.230 kb on - strand, within GFF3975 at 179.241 kb on + strand, within GFF3975 at 179.241 kb on + strand, within GFF3975 at 179.242 kb on - strand, within GFF3975 at 179.242 kb on - strand, within GFF3975 at 179.242 kb on - strand, within GFF3975 at 179.244 kb on + strand, within GFF3975 at 179.244 kb on + strand, within GFF3975 at 179.245 kb on - strand, within GFF3975 at 179.245 kb on - strand, within GFF3975 at 179.245 kb on - strand, within GFF3975 at 179.245 kb on - strand, within GFF3975
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 176,565 + GFF3973 0.36 -1.5 176,566 - GFF3973 0.36 +0.3 176,613 + GFF3973 0.40 -0.3 176,613 + GFF3973 0.40 +1.1 176,614 - GFF3973 0.40 -0.9 176,614 - GFF3973 0.40 +0.1 176,614 - GFF3973 0.40 -1.3 176,626 - GFF3973 0.41 -0.8 176,730 + GFF3973 0.50 +0.5 176,731 - GFF3973 0.50 +0.2 176,731 - GFF3973 0.50 +0.4 176,947 - GFF3973 0.68 +0.6 176,994 + GFF3973 0.72 -0.5 176,994 + GFF3973 0.72 -1.2 176,994 + GFF3973 0.72 -1.1 176,995 - GFF3973 0.72 -0.2 177,270 + -0.9 177,270 + +0.6 177,271 - -1.1 177,333 + +0.0 177,333 + +0.4 177,334 - -1.0 178,145 + GFF3974 0.66 -0.8 178,145 + GFF3974 0.66 -1.0 178,146 - GFF3974 0.66 -1.0 178,146 - GFF3974 0.66 +0.4 178,485 - +0.3 178,968 + GFF3975 0.47 -0.9 178,968 + GFF3975 0.47 -0.9 179,212 - GFF3975 0.77 -1.0 179,212 - GFF3975 0.77 +0.4 179,230 - GFF3975 0.79 -0.2 179,241 + GFF3975 0.80 -0.3 179,241 + GFF3975 0.80 -2.1 179,242 - GFF3975 0.81 -0.2 179,242 - GFF3975 0.81 -0.1 179,242 - GFF3975 0.81 +0.0 179,244 + GFF3975 0.81 -0.3 179,244 + GFF3975 0.81 +0.9 179,245 - GFF3975 0.81 -0.0 179,245 - GFF3975 0.81 +1.2 179,245 - GFF3975 0.81 -1.6 179,245 - GFF3975 0.81 +0.9
Or see this region's nucleotide sequence