Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3700 and GFF3701 are separated by 126 nucleotides GFF3701 and GFF3702 are separated by 5 nucleotides GFF3702 and GFF3703 are separated by 126 nucleotides GFF3703 and GFF3704 are separated by 45 nucleotides
GFF3700 - FIG006285: hypothetical protein, at 201,318 to 202,016
GFF3700
GFF3701 - FIG004694: Hypothetical protein, at 202,143 to 202,670
GFF3701
GFF3702 - Beta-propeller domains of methanol dehydrogenase type, at 202,676 to 203,533
GFF3702
GFF3703 - LemA family protein, at 203,660 to 204,259
GFF3703
GFF3704 - FIG00536957: hypothetical protein, at 204,305 to 205,063
GFF3704
Position (kb) 202
203
204 Strain fitness (log2 ratio) -2
-1
0
1
2 at 202.152 kb on + strand at 202.152 kb on + strand at 202.152 kb on + strand at 202.153 kb on - strand at 202.153 kb on - strand at 202.153 kb on - strand at 202.384 kb on + strand, within GFF3701 at 202.384 kb on + strand, within GFF3701 at 202.384 kb on + strand, within GFF3701 at 202.384 kb on + strand, within GFF3701 at 202.384 kb on + strand, within GFF3701 at 202.385 kb on - strand, within GFF3701 at 202.385 kb on - strand, within GFF3701 at 202.385 kb on - strand, within GFF3701 at 202.385 kb on - strand, within GFF3701 at 202.480 kb on + strand, within GFF3701 at 202.480 kb on + strand, within GFF3701 at 202.481 kb on - strand, within GFF3701 at 202.481 kb on - strand, within GFF3701 at 202.481 kb on - strand, within GFF3701 at 203.328 kb on + strand, within GFF3702 at 203.661 kb on + strand at 203.745 kb on + strand, within GFF3703 at 203.800 kb on - strand, within GFF3703 at 203.800 kb on - strand, within GFF3703 at 203.800 kb on - strand, within GFF3703 at 203.800 kb on - strand, within GFF3703 at 203.800 kb on - strand, within GFF3703 at 203.800 kb on - strand, within GFF3703 at 203.800 kb on - strand, within GFF3703 at 203.820 kb on + strand, within GFF3703 at 204.136 kb on - strand, within GFF3703 at 204.198 kb on + strand, within GFF3703 at 204.199 kb on - strand, within GFF3703 at 204.199 kb on - strand, within GFF3703 at 204.266 kb on + strand at 204.266 kb on + strand at 204.267 kb on - strand at 204.267 kb on - strand at 204.290 kb on + strand at 204.290 kb on + strand at 204.290 kb on + strand at 204.290 kb on + strand at 204.290 kb on + strand at 204.295 kb on + strand at 204.295 kb on + strand at 204.295 kb on + strand at 204.295 kb on + strand at 204.295 kb on + strand at 204.295 kb on + strand at 204.295 kb on + strand at 204.295 kb on + strand at 204.295 kb on + strand at 204.296 kb on - strand at 204.296 kb on - strand at 204.296 kb on - strand at 204.296 kb on - strand at 204.296 kb on - strand at 204.296 kb on - strand at 204.296 kb on - strand at 204.296 kb on - strand at 204.296 kb on - strand at 204.296 kb on - strand at 204.296 kb on - strand at 204.296 kb on - strand at 204.296 kb on - strand at 204.296 kb on - strand at 204.296 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 202,152 + -0.7 202,152 + +0.7 202,152 + -0.4 202,153 - -0.8 202,153 - +0.9 202,153 - -0.4 202,384 + GFF3701 0.46 +1.4 202,384 + GFF3701 0.46 -1.0 202,384 + GFF3701 0.46 +0.2 202,384 + GFF3701 0.46 +0.3 202,384 + GFF3701 0.46 -1.5 202,385 - GFF3701 0.46 +0.9 202,385 - GFF3701 0.46 -0.0 202,385 - GFF3701 0.46 -0.5 202,385 - GFF3701 0.46 -2.3 202,480 + GFF3701 0.64 +0.1 202,480 + GFF3701 0.64 -0.3 202,481 - GFF3701 0.64 +0.5 202,481 - GFF3701 0.64 -0.4 202,481 - GFF3701 0.64 +0.9 203,328 + GFF3702 0.76 +0.5 203,661 + -2.7 203,745 + GFF3703 0.14 -0.5 203,800 - GFF3703 0.23 -0.5 203,800 - GFF3703 0.23 +1.4 203,800 - GFF3703 0.23 -0.6 203,800 - GFF3703 0.23 -1.3 203,800 - GFF3703 0.23 -0.2 203,800 - GFF3703 0.23 +0.3 203,800 - GFF3703 0.23 -0.6 203,820 + GFF3703 0.27 +0.1 204,136 - GFF3703 0.79 -2.1 204,198 + GFF3703 0.90 +2.3 204,199 - GFF3703 0.90 +1.6 204,199 - GFF3703 0.90 -1.4 204,266 + -1.7 204,266 + +0.6 204,267 - +0.3 204,267 - +0.5 204,290 + +1.0 204,290 + -1.0 204,290 + +0.3 204,290 + -0.2 204,290 + -0.0 204,295 + -0.6 204,295 + -0.1 204,295 + -1.5 204,295 + +0.3 204,295 + -0.5 204,295 + +0.5 204,295 + -2.2 204,295 + +0.4 204,295 + -0.9 204,296 - +0.2 204,296 - -0.3 204,296 - -0.3 204,296 - -0.5 204,296 - +0.9 204,296 - +1.0 204,296 - -0.7 204,296 - -0.8 204,296 - +1.2 204,296 - +0.2 204,296 - -0.3 204,296 - +0.3 204,296 - -0.9 204,296 - -0.3 204,296 - +0.1
Or see this region's nucleotide sequence