Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3436 and GFF3437 are separated by 127 nucleotides GFF3437 and GFF3438 are separated by 149 nucleotides
GFF3436 - Transcriptional regulator, LysR family, at 258,464 to 259,432
GFF3436
GFF3437 - putative membrane protein, at 259,560 to 260,903
GFF3437
GFF3438 - Amidohydrolase family protein, at 261,053 to 262,222
GFF3438
Position (kb)
259
260
261 Strain fitness (log2 ratio)
-2
-1
0
1 at 258.708 kb on + strand, within GFF3436 at 258.708 kb on + strand, within GFF3436 at 259.119 kb on + strand, within GFF3436 at 259.119 kb on + strand, within GFF3436 at 259.120 kb on - strand, within GFF3436 at 259.221 kb on + strand, within GFF3436 at 259.221 kb on + strand, within GFF3436 at 259.222 kb on - strand, within GFF3436 at 259.399 kb on - strand at 259.399 kb on - strand at 259.483 kb on - strand at 259.509 kb on + strand at 259.509 kb on + strand at 260.055 kb on + strand, within GFF3437 at 260.055 kb on + strand, within GFF3437 at 260.055 kb on + strand, within GFF3437 at 260.055 kb on + strand, within GFF3437 at 260.056 kb on - strand, within GFF3437 at 260.056 kb on - strand, within GFF3437 at 260.056 kb on - strand, within GFF3437 at 260.097 kb on + strand, within GFF3437 at 260.097 kb on + strand, within GFF3437 at 260.098 kb on - strand, within GFF3437 at 260.098 kb on - strand, within GFF3437 at 260.098 kb on - strand, within GFF3437 at 260.098 kb on - strand, within GFF3437 at 260.098 kb on - strand, within GFF3437 at 260.328 kb on + strand, within GFF3437 at 260.329 kb on - strand, within GFF3437 at 260.329 kb on - strand, within GFF3437 at 260.370 kb on + strand, within GFF3437 at 260.547 kb on + strand, within GFF3437 at 260.547 kb on + strand, within GFF3437 at 260.547 kb on + strand, within GFF3437 at 260.547 kb on + strand, within GFF3437 at 260.547 kb on + strand, within GFF3437 at 260.548 kb on - strand, within GFF3437 at 260.548 kb on - strand, within GFF3437 at 260.548 kb on - strand, within GFF3437 at 260.548 kb on - strand, within GFF3437 at 260.548 kb on - strand, within GFF3437 at 260.869 kb on + strand at 260.870 kb on - strand at 261.057 kb on - strand at 261.057 kb on - strand at 261.057 kb on - strand at 261.466 kb on - strand, within GFF3438 at 261.778 kb on + strand, within GFF3438
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 258,708 + GFF3436 0.25 -0.3 258,708 + GFF3436 0.25 -0.2 259,119 + GFF3436 0.68 -0.3 259,119 + GFF3436 0.68 -0.1 259,120 - GFF3436 0.68 -0.3 259,221 + GFF3436 0.78 -0.4 259,221 + GFF3436 0.78 -0.3 259,222 - GFF3436 0.78 +0.6 259,399 - +0.4 259,399 - +0.7 259,483 - +0.9 259,509 + -0.8 259,509 + +0.1 260,055 + GFF3437 0.37 -0.6 260,055 + GFF3437 0.37 -0.6 260,055 + GFF3437 0.37 +1.6 260,055 + GFF3437 0.37 -0.2 260,056 - GFF3437 0.37 -1.6 260,056 - GFF3437 0.37 -0.1 260,056 - GFF3437 0.37 -1.1 260,097 + GFF3437 0.40 +0.3 260,097 + GFF3437 0.40 -0.9 260,098 - GFF3437 0.40 +0.0 260,098 - GFF3437 0.40 -1.2 260,098 - GFF3437 0.40 -0.3 260,098 - GFF3437 0.40 +0.3 260,098 - GFF3437 0.40 -0.0 260,328 + GFF3437 0.57 -0.7 260,329 - GFF3437 0.57 -0.4 260,329 - GFF3437 0.57 +0.1 260,370 + GFF3437 0.60 -2.2 260,547 + GFF3437 0.73 -0.2 260,547 + GFF3437 0.73 +0.9 260,547 + GFF3437 0.73 +0.6 260,547 + GFF3437 0.73 -0.0 260,547 + GFF3437 0.73 -1.2 260,548 - GFF3437 0.74 +0.1 260,548 - GFF3437 0.74 +0.1 260,548 - GFF3437 0.74 -0.1 260,548 - GFF3437 0.74 -0.7 260,548 - GFF3437 0.74 +0.1 260,869 + -1.0 260,870 - -0.1 261,057 - -2.2 261,057 - +0.2 261,057 - -1.2 261,466 - GFF3438 0.35 -0.9 261,778 + GFF3438 0.62 -1.4
Or see this region's nucleotide sequence