Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3320 and GFF3321 overlap by 4 nucleotides GFF3321 and GFF3322 are separated by 34 nucleotides GFF3322 and GFF3323 are separated by 181 nucleotides GFF3323 and GFF3324 are separated by 7 nucleotides
GFF3320 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179), at 147,272 to 148,555
GFF3320
GFF3321 - Acyl carrier protein, at 148,552 to 148,857
GFF3321
GFF3322 - hypothetical protein, at 148,892 to 149,752
GFF3322
GFF3323 - Tricarboxylate transport transcriptional regulator TctD, at 149,934 to 150,578
GFF3323
GFF3324 - hypothetical protein, at 150,586 to 151,980
GFF3324
Position (kb) 148
149
150 Strain fitness (log2 ratio) -2
-1
0
1
2 at 148.143 kb on + strand, within GFF3320 at 148.144 kb on - strand, within GFF3320 at 148.560 kb on + strand at 148.561 kb on - strand at 148.899 kb on + strand at 148.899 kb on + strand at 148.900 kb on - strand at 148.900 kb on - strand at 149.001 kb on + strand, within GFF3322 at 149.001 kb on + strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.277 kb on + strand, within GFF3322 at 149.295 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.583 kb on + strand, within GFF3322 at 149.584 kb on - strand, within GFF3322 at 149.613 kb on + strand, within GFF3322 at 149.789 kb on + strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.800 kb on + strand at 149.800 kb on + strand at 149.800 kb on + strand at 149.801 kb on - strand at 149.801 kb on - strand at 149.801 kb on - strand at 149.868 kb on + strand at 150.555 kb on + strand at 150.555 kb on + strand at 150.725 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 148,143 + GFF3320 0.68 -1.1 148,144 - GFF3320 0.68 -0.4 148,560 + +0.1 148,561 - +1.5 148,899 + -1.4 148,899 + +0.1 148,900 - -0.9 148,900 - -0.6 149,001 + GFF3322 0.13 +0.4 149,001 + GFF3322 0.13 -1.6 149,002 - GFF3322 0.13 +0.9 149,002 - GFF3322 0.13 +0.9 149,002 - GFF3322 0.13 -0.7 149,002 - GFF3322 0.13 +0.6 149,002 - GFF3322 0.13 +0.6 149,277 + GFF3322 0.45 -0.1 149,295 + GFF3322 0.47 -0.4 149,322 + GFF3322 0.50 +0.4 149,322 + GFF3322 0.50 +0.7 149,322 + GFF3322 0.50 -0.8 149,322 + GFF3322 0.50 -0.3 149,322 + GFF3322 0.50 -0.8 149,322 + GFF3322 0.50 -0.1 149,323 - GFF3322 0.50 -0.1 149,323 - GFF3322 0.50 -0.3 149,323 - GFF3322 0.50 -0.2 149,323 - GFF3322 0.50 +0.4 149,323 - GFF3322 0.50 -0.5 149,583 + GFF3322 0.80 +1.0 149,584 - GFF3322 0.80 +0.5 149,613 + GFF3322 0.84 +0.1 149,789 + -0.9 149,790 - +0.3 149,790 - -1.3 149,790 - +0.3 149,790 - -1.6 149,790 - +0.3 149,790 - +0.9 149,790 - -1.1 149,800 + +0.6 149,800 + -0.5 149,800 + +1.9 149,801 - +1.1 149,801 - -0.8 149,801 - -0.4 149,868 + -0.2 150,555 + +0.3 150,555 + -0.3 150,725 - -2.3
Or see this region's nucleotide sequence