Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF307 and GFF308 are separated by 520 nucleotides GFF308 and GFF309 overlap by 4 nucleotides GFF309 and GFF310 overlap by 4 nucleotides
GFF307 - FIG143263: Glycosyl transferase, at 340,034 to 340,810
GFF307
GFF308 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 341,331 to 342,062
GFF308
GFF309 - 3-hydroxydecanoyl-[ACP] dehydratase (EC 4.2.1.59), at 342,059 to 342,532
GFF309
GFF310 - FIG025233: SAM-dependent methyltransferases, at 342,529 to 343,305
GFF310
Position (kb) 341
342
343 Strain fitness (log2 ratio) -2
-1
0
1
2 at 340.470 kb on + strand, within GFF307 at 340.470 kb on + strand, within GFF307 at 340.470 kb on + strand, within GFF307 at 340.470 kb on + strand, within GFF307 at 340.471 kb on - strand, within GFF307 at 340.471 kb on - strand, within GFF307 at 340.471 kb on - strand, within GFF307 at 340.505 kb on + strand, within GFF307 at 340.506 kb on - strand, within GFF307 at 340.652 kb on + strand, within GFF307 at 340.652 kb on + strand, within GFF307 at 340.652 kb on + strand, within GFF307 at 340.652 kb on + strand, within GFF307 at 340.653 kb on - strand, within GFF307 at 340.653 kb on - strand, within GFF307 at 340.840 kb on - strand at 340.846 kb on + strand at 341.374 kb on + strand at 341.374 kb on + strand at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 342.163 kb on - strand, within GFF309 at 342.163 kb on - strand, within GFF309 at 342.342 kb on + strand, within GFF309 at 342.342 kb on + strand, within GFF309 at 342.343 kb on - strand, within GFF309 at 342.851 kb on + strand, within GFF310 at 342.851 kb on + strand, within GFF310 at 342.852 kb on - strand, within GFF310 at 342.852 kb on - strand, within GFF310 at 342.852 kb on - strand, within GFF310 at 343.001 kb on + strand, within GFF310
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 340,470 + GFF307 0.56 -0.0 340,470 + GFF307 0.56 +0.5 340,470 + GFF307 0.56 +0.5 340,470 + GFF307 0.56 +0.1 340,471 - GFF307 0.56 -0.8 340,471 - GFF307 0.56 -1.5 340,471 - GFF307 0.56 -0.2 340,505 + GFF307 0.61 +0.5 340,506 - GFF307 0.61 +0.1 340,652 + GFF307 0.80 -0.5 340,652 + GFF307 0.80 +0.0 340,652 + GFF307 0.80 -0.4 340,652 + GFF307 0.80 +0.7 340,653 - GFF307 0.80 -0.4 340,653 - GFF307 0.80 -1.3 340,840 - -0.5 340,846 + -0.8 341,374 + -0.1 341,374 + +0.3 341,602 + GFF308 0.37 +0.0 341,602 + GFF308 0.37 -0.0 341,602 + GFF308 0.37 -0.2 341,602 + GFF308 0.37 -0.1 341,602 + GFF308 0.37 +0.7 341,602 + GFF308 0.37 -0.1 341,602 + GFF308 0.37 +0.2 341,602 + GFF308 0.37 +0.7 341,602 + GFF308 0.37 -0.6 341,603 - GFF308 0.37 -0.0 341,603 - GFF308 0.37 +0.3 341,603 - GFF308 0.37 +2.2 341,603 - GFF308 0.37 +0.5 341,603 - GFF308 0.37 +0.5 341,603 - GFF308 0.37 +1.2 341,603 - GFF308 0.37 -1.2 342,163 - GFF309 0.22 -2.3 342,163 - GFF309 0.22 -1.8 342,342 + GFF309 0.60 +0.0 342,342 + GFF309 0.60 -0.6 342,343 - GFF309 0.60 -0.5 342,851 + GFF310 0.41 +0.5 342,851 + GFF310 0.41 +0.4 342,852 - GFF310 0.42 +0.4 342,852 - GFF310 0.42 -0.5 342,852 - GFF310 0.42 +0.5 343,001 + GFF310 0.61 +1.2
Or see this region's nucleotide sequence