Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2766 and GFF2767 are separated by 9 nucleotides GFF2767 and GFF2768 are separated by 38 nucleotides GFF2768 and GFF2769 are separated by 92 nucleotides GFF2769 and GFF2770 are separated by 75 nucleotides
GFF2766 - Pentapeptide repeat family protein, at 196,869 to 197,555
GFF2766
GFF2767 - MaoC domain protein dehydratase, at 197,565 to 198,044
GFF2767
GFF2768 - 3-ketoacyl-CoA thiolase 2 [fadN-fadA-fadE operon] (EC 2.3.1.16), at 198,083 to 199,216
GFF2768
GFF2769 - Transcriptional regulator, AcrR family, at 199,309 to 199,896
GFF2769
GFF2770 - BUG/TctC family periplasmic protein, at 199,972 to 200,964
GFF2770
Position (kb) 198
199
200 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 198.004 kb on + strand at 198.004 kb on + strand at 198.005 kb on - strand at 198.005 kb on - strand at 198.005 kb on - strand at 198.005 kb on - strand at 198.005 kb on - strand at 198.147 kb on + strand at 198.792 kb on + strand, within GFF2768 at 198.792 kb on + strand, within GFF2768 at 198.792 kb on + strand, within GFF2768 at 198.793 kb on - strand, within GFF2768 at 198.793 kb on - strand, within GFF2768 at 198.793 kb on - strand, within GFF2768 at 198.793 kb on - strand, within GFF2768 at 198.793 kb on - strand, within GFF2768 at 198.793 kb on - strand, within GFF2768 at 198.793 kb on - strand, within GFF2768 at 198.793 kb on - strand, within GFF2768 at 198.793 kb on - strand, within GFF2768 at 198.793 kb on - strand, within GFF2768 at 198.793 kb on - strand, within GFF2768 at 198.793 kb on - strand, within GFF2768 at 198.793 kb on - strand, within GFF2768 at 199.047 kb on + strand, within GFF2768 at 199.234 kb on - strand at 199.415 kb on + strand, within GFF2769 at 199.415 kb on + strand, within GFF2769 at 199.415 kb on + strand, within GFF2769 at 199.415 kb on + strand, within GFF2769 at 199.415 kb on + strand, within GFF2769 at 199.415 kb on + strand, within GFF2769 at 199.415 kb on + strand, within GFF2769 at 199.415 kb on + strand, within GFF2769 at 199.415 kb on + strand, within GFF2769 at 199.415 kb on + strand, within GFF2769 at 199.416 kb on - strand, within GFF2769 at 199.416 kb on - strand, within GFF2769 at 199.416 kb on - strand, within GFF2769 at 199.416 kb on - strand, within GFF2769 at 199.416 kb on - strand, within GFF2769 at 199.416 kb on - strand, within GFF2769 at 199.416 kb on - strand, within GFF2769 at 199.416 kb on - strand, within GFF2769 at 199.416 kb on - strand, within GFF2769 at 199.416 kb on - strand, within GFF2769 at 199.416 kb on - strand, within GFF2769 at 199.610 kb on + strand, within GFF2769 at 199.610 kb on + strand, within GFF2769 at 199.610 kb on + strand, within GFF2769 at 199.610 kb on + strand, within GFF2769 at 199.610 kb on + strand, within GFF2769 at 199.611 kb on - strand, within GFF2769 at 199.611 kb on - strand, within GFF2769 at 199.611 kb on - strand, within GFF2769 at 199.611 kb on - strand, within GFF2769 at 199.739 kb on + strand, within GFF2769 at 199.739 kb on + strand, within GFF2769 at 199.739 kb on + strand, within GFF2769 at 199.740 kb on - strand, within GFF2769 at 199.740 kb on - strand, within GFF2769 at 199.740 kb on - strand, within GFF2769 at 199.740 kb on - strand, within GFF2769 at 199.799 kb on + strand, within GFF2769 at 199.800 kb on - strand, within GFF2769 at 199.898 kb on + strand at 199.898 kb on + strand at 199.898 kb on + strand at 199.899 kb on - strand at 199.899 kb on - strand at 199.899 kb on - strand at 199.902 kb on + strand at 199.906 kb on - strand at 199.906 kb on - strand at 199.907 kb on + strand at 199.907 kb on + strand at 199.908 kb on - strand at 199.908 kb on - strand at 199.908 kb on - strand at 200.036 kb on + strand at 200.141 kb on + strand, within GFF2770 at 200.141 kb on + strand, within GFF2770 at 200.141 kb on + strand, within GFF2770 at 200.142 kb on - strand, within GFF2770 at 200.142 kb on - strand, within GFF2770 at 200.142 kb on - strand, within GFF2770 at 200.142 kb on - strand, within GFF2770 at 200.216 kb on + strand, within GFF2770 at 200.216 kb on + strand, within GFF2770 at 200.216 kb on + strand, within GFF2770
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 198,004 + -0.7 198,004 + -0.2 198,005 - -1.2 198,005 - +0.0 198,005 - -2.8 198,005 - -1.0 198,005 - -1.1 198,147 + +0.6 198,792 + GFF2768 0.63 -1.0 198,792 + GFF2768 0.63 -0.4 198,792 + GFF2768 0.63 +0.9 198,793 - GFF2768 0.63 -1.2 198,793 - GFF2768 0.63 +0.4 198,793 - GFF2768 0.63 +0.2 198,793 - GFF2768 0.63 +1.1 198,793 - GFF2768 0.63 +0.4 198,793 - GFF2768 0.63 -0.4 198,793 - GFF2768 0.63 -0.4 198,793 - GFF2768 0.63 +0.2 198,793 - GFF2768 0.63 +0.3 198,793 - GFF2768 0.63 -2.7 198,793 - GFF2768 0.63 +0.6 198,793 - GFF2768 0.63 +0.0 198,793 - GFF2768 0.63 -0.5 199,047 + GFF2768 0.85 +0.1 199,234 - +1.1 199,415 + GFF2769 0.18 -1.0 199,415 + GFF2769 0.18 -0.2 199,415 + GFF2769 0.18 -0.4 199,415 + GFF2769 0.18 -1.4 199,415 + GFF2769 0.18 -1.0 199,415 + GFF2769 0.18 +0.1 199,415 + GFF2769 0.18 -0.5 199,415 + GFF2769 0.18 -0.1 199,415 + GFF2769 0.18 -1.4 199,415 + GFF2769 0.18 +0.1 199,416 - GFF2769 0.18 +0.2 199,416 - GFF2769 0.18 -0.7 199,416 - GFF2769 0.18 +1.1 199,416 - GFF2769 0.18 +0.9 199,416 - GFF2769 0.18 -0.9 199,416 - GFF2769 0.18 -0.9 199,416 - GFF2769 0.18 +0.1 199,416 - GFF2769 0.18 -0.7 199,416 - GFF2769 0.18 +0.3 199,416 - GFF2769 0.18 -0.2 199,416 - GFF2769 0.18 +1.6 199,610 + GFF2769 0.51 -0.1 199,610 + GFF2769 0.51 -0.3 199,610 + GFF2769 0.51 -0.7 199,610 + GFF2769 0.51 +1.1 199,610 + GFF2769 0.51 -0.8 199,611 - GFF2769 0.51 -0.5 199,611 - GFF2769 0.51 -0.3 199,611 - GFF2769 0.51 -1.1 199,611 - GFF2769 0.51 -0.7 199,739 + GFF2769 0.73 -0.9 199,739 + GFF2769 0.73 -1.7 199,739 + GFF2769 0.73 -0.2 199,740 - GFF2769 0.73 -0.4 199,740 - GFF2769 0.73 -0.6 199,740 - GFF2769 0.73 -0.2 199,740 - GFF2769 0.73 +0.6 199,799 + GFF2769 0.83 -1.1 199,800 - GFF2769 0.84 -0.4 199,898 + -0.2 199,898 + -2.4 199,898 + -1.7 199,899 - -0.8 199,899 - -0.1 199,899 - -0.2 199,902 + -1.4 199,906 - +0.4 199,906 - -0.2 199,907 + -1.4 199,907 + -0.3 199,908 - -1.1 199,908 - -0.2 199,908 - -0.1 200,036 + -1.1 200,141 + GFF2770 0.17 -0.2 200,141 + GFF2770 0.17 -0.2 200,141 + GFF2770 0.17 +1.2 200,142 - GFF2770 0.17 +1.3 200,142 - GFF2770 0.17 +0.3 200,142 - GFF2770 0.17 +0.4 200,142 - GFF2770 0.17 -1.9 200,216 + GFF2770 0.25 +0.8 200,216 + GFF2770 0.25 +0.8 200,216 + GFF2770 0.25 -0.4
Or see this region's nucleotide sequence