Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2340 and GFF2341 are separated by 120 nucleotides GFF2341 and GFF2342 overlap by 4 nucleotides GFF2342 and GFF2343 are separated by 5 nucleotides GFF2343 and GFF2344 overlap by 4 nucleotides
GFF2340 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 391,246 to 392,739
GFF2340
GFF2341 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 392,860 to 393,690
GFF2341
GFF2342 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 393,687 to 394,676
GFF2342
GFF2343 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 394,682 to 395,557
GFF2343
GFF2344 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 395,554 to 396,504
GFF2344
Position (kb) 393
394
395 Strain fitness (log2 ratio) -2
-1
0
1
2 at 392.810 kb on - strand at 392.825 kb on + strand at 392.924 kb on + strand at 393.369 kb on - strand, within GFF2341 at 393.632 kb on + strand at 393.632 kb on + strand at 393.802 kb on + strand, within GFF2342 at 393.802 kb on + strand, within GFF2342 at 393.803 kb on - strand, within GFF2342 at 393.803 kb on - strand, within GFF2342 at 393.803 kb on - strand, within GFF2342 at 393.803 kb on - strand, within GFF2342 at 393.803 kb on - strand, within GFF2342 at 393.934 kb on + strand, within GFF2342 at 393.935 kb on - strand, within GFF2342 at 393.997 kb on + strand, within GFF2342 at 393.998 kb on - strand, within GFF2342 at 394.609 kb on - strand at 394.683 kb on + strand at 394.683 kb on + strand at 394.684 kb on - strand at 394.684 kb on - strand at 395.250 kb on - strand, within GFF2343 at 395.334 kb on + strand, within GFF2343 at 395.334 kb on + strand, within GFF2343 at 395.335 kb on - strand, within GFF2343 at 395.335 kb on - strand, within GFF2343 at 395.558 kb on - strand at 395.558 kb on - strand at 395.558 kb on - strand at 395.558 kb on - strand at 395.558 kb on - strand at 395.558 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 392,810 - -2.4 392,825 + +0.9 392,924 + -1.0 393,369 - GFF2341 0.61 -0.2 393,632 + -0.2 393,632 + -0.9 393,802 + GFF2342 0.12 +1.7 393,802 + GFF2342 0.12 -1.3 393,803 - GFF2342 0.12 +0.4 393,803 - GFF2342 0.12 -1.5 393,803 - GFF2342 0.12 +0.0 393,803 - GFF2342 0.12 +0.7 393,803 - GFF2342 0.12 +0.2 393,934 + GFF2342 0.25 +1.0 393,935 - GFF2342 0.25 -0.3 393,997 + GFF2342 0.31 +2.3 393,998 - GFF2342 0.31 -1.5 394,609 - +0.6 394,683 + +1.8 394,683 + -0.4 394,684 - -0.4 394,684 - +0.3 395,250 - GFF2343 0.65 -2.0 395,334 + GFF2343 0.74 -0.9 395,334 + GFF2343 0.74 -0.1 395,335 - GFF2343 0.75 +0.2 395,335 - GFF2343 0.75 -0.6 395,558 - -0.1 395,558 - -0.5 395,558 - +0.8 395,558 - -0.9 395,558 - +1.2 395,558 - -0.3
Or see this region's nucleotide sequence