Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF223 and GFF224 are separated by 15 nucleotides GFF224 and GFF225 are separated by 189 nucleotides GFF225 and GFF226 are separated by 16 nucleotides
GFF223 - Transcription termination protein NusA, at 249,281 to 250,774
GFF223
GFF224 - Bacterial ribosome SSU maturation protein RimP, at 250,790 to 251,449
GFF224
GFF225 - Permeases of the major facilitator superfamily, at 251,639 to 252,835
GFF225
GFF226 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines) / ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 252,852 to 254,492
GFF226
Position (kb) 251
252
253 Strain fitness (log2 ratio) -2
-1
0
1
2 at 251.470 kb on - strand at 251.521 kb on - strand at 251.521 kb on - strand at 251.521 kb on - strand at 251.521 kb on - strand at 251.546 kb on - strand at 251.546 kb on - strand at 251.546 kb on - strand at 251.546 kb on - strand at 251.546 kb on - strand at 251.546 kb on - strand at 251.546 kb on - strand at 251.546 kb on - strand at 251.546 kb on - strand at 251.546 kb on - strand at 251.571 kb on - strand at 251.603 kb on - strand at 251.603 kb on - strand at 251.603 kb on - strand at 251.603 kb on - strand at 251.603 kb on - strand at 251.608 kb on - strand at 251.608 kb on - strand at 251.608 kb on - strand at 251.608 kb on - strand at 251.619 kb on - strand at 251.649 kb on - strand at 251.649 kb on - strand at 251.649 kb on - strand at 251.649 kb on - strand at 251.685 kb on - strand at 251.685 kb on - strand at 251.685 kb on - strand at 251.685 kb on - strand at 251.800 kb on - strand, within GFF225 at 251.815 kb on - strand, within GFF225 at 251.815 kb on - strand, within GFF225 at 251.815 kb on - strand, within GFF225 at 251.815 kb on - strand, within GFF225 at 251.980 kb on - strand, within GFF225 at 251.980 kb on - strand, within GFF225 at 252.079 kb on - strand, within GFF225 at 252.140 kb on - strand, within GFF225 at 252.140 kb on - strand, within GFF225 at 252.148 kb on - strand, within GFF225 at 252.148 kb on - strand, within GFF225 at 252.148 kb on - strand, within GFF225 at 252.148 kb on - strand, within GFF225 at 252.148 kb on - strand, within GFF225 at 252.169 kb on - strand, within GFF225 at 252.411 kb on + strand, within GFF225 at 252.411 kb on + strand, within GFF225 at 252.412 kb on - strand, within GFF225 at 252.412 kb on - strand, within GFF225 at 252.412 kb on - strand, within GFF225 at 252.412 kb on - strand, within GFF225 at 252.412 kb on - strand, within GFF225 at 252.412 kb on - strand, within GFF225 at 252.651 kb on + strand, within GFF225 at 252.651 kb on + strand, within GFF225 at 252.651 kb on + strand, within GFF225 at 252.652 kb on - strand, within GFF225 at 252.652 kb on - strand, within GFF225 at 252.660 kb on + strand, within GFF225 at 252.660 kb on + strand, within GFF225 at 252.660 kb on + strand, within GFF225 at 252.660 kb on + strand, within GFF225 at 252.660 kb on + strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.853 kb on + strand at 252.853 kb on + strand at 252.887 kb on + strand at 252.887 kb on + strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.890 kb on - strand at 252.890 kb on - strand at 252.890 kb on - strand at 252.890 kb on - strand at 253.015 kb on + strand at 253.015 kb on + strand at 253.015 kb on + strand at 253.015 kb on + strand at 253.015 kb on + strand at 253.015 kb on + strand at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.296 kb on + strand, within GFF226 at 253.296 kb on + strand, within GFF226 at 253.297 kb on - strand, within GFF226 at 253.481 kb on - strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.613 kb on - strand, within GFF226 at 253.613 kb on - strand, within GFF226 at 253.718 kb on - strand, within GFF226 at 253.718 kb on - strand, within GFF226 at 253.718 kb on - strand, within GFF226
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 251,470 - -0.8 251,521 - -1.4 251,521 - +1.9 251,521 - -0.4 251,521 - +0.3 251,546 - -0.9 251,546 - +0.2 251,546 - +0.2 251,546 - -0.7 251,546 - -0.7 251,546 - -0.8 251,546 - -0.6 251,546 - -0.3 251,546 - +0.2 251,546 - +0.1 251,571 - +0.7 251,603 - -0.4 251,603 - -0.5 251,603 - +0.6 251,603 - -2.1 251,603 - +0.4 251,608 - -0.4 251,608 - -1.9 251,608 - -0.0 251,608 - -0.2 251,619 - -0.1 251,649 - +0.6 251,649 - -0.1 251,649 - -0.1 251,649 - -1.9 251,685 - -0.4 251,685 - +0.3 251,685 - -0.4 251,685 - -0.7 251,800 - GFF225 0.13 +0.2 251,815 - GFF225 0.15 -1.2 251,815 - GFF225 0.15 -0.5 251,815 - GFF225 0.15 +0.3 251,815 - GFF225 0.15 -1.0 251,980 - GFF225 0.28 +0.9 251,980 - GFF225 0.28 -0.7 252,079 - GFF225 0.37 +0.3 252,140 - GFF225 0.42 -0.3 252,140 - GFF225 0.42 +1.1 252,148 - GFF225 0.43 -0.1 252,148 - GFF225 0.43 -1.3 252,148 - GFF225 0.43 -1.7 252,148 - GFF225 0.43 -0.7 252,148 - GFF225 0.43 +0.5 252,169 - GFF225 0.44 -0.6 252,411 + GFF225 0.64 -1.4 252,411 + GFF225 0.64 -1.4 252,412 - GFF225 0.65 -0.3 252,412 - GFF225 0.65 -1.1 252,412 - GFF225 0.65 -1.9 252,412 - GFF225 0.65 +0.5 252,412 - GFF225 0.65 -1.5 252,412 - GFF225 0.65 +0.4 252,651 + GFF225 0.85 +0.3 252,651 + GFF225 0.85 -1.0 252,651 + GFF225 0.85 -1.1 252,652 - GFF225 0.85 -0.4 252,652 - GFF225 0.85 +0.1 252,660 + GFF225 0.85 -1.4 252,660 + GFF225 0.85 +0.1 252,660 + GFF225 0.85 -0.8 252,660 + GFF225 0.85 -0.2 252,660 + GFF225 0.85 -0.1 252,661 - GFF225 0.85 -0.9 252,661 - GFF225 0.85 +0.3 252,661 - GFF225 0.85 -1.4 252,661 - GFF225 0.85 -0.0 252,661 - GFF225 0.85 -0.8 252,661 - GFF225 0.85 -0.5 252,661 - GFF225 0.85 -0.7 252,853 + -1.4 252,853 + +1.0 252,887 + -0.1 252,887 + +1.9 252,888 - -0.1 252,888 - +0.4 252,888 - -0.8 252,888 - -2.2 252,888 - +0.2 252,888 - -0.5 252,888 - +0.0 252,888 - +0.8 252,890 - +0.1 252,890 - -0.7 252,890 - -0.0 252,890 - -0.3 253,015 + -1.4 253,015 + -0.8 253,015 + -0.8 253,015 + -1.0 253,015 + -1.5 253,015 + +0.7 253,016 - GFF226 0.10 +1.1 253,016 - GFF226 0.10 -2.3 253,016 - GFF226 0.10 -1.3 253,016 - GFF226 0.10 -1.3 253,016 - GFF226 0.10 -1.0 253,016 - GFF226 0.10 -1.0 253,016 - GFF226 0.10 -1.5 253,016 - GFF226 0.10 -0.0 253,296 + GFF226 0.27 -1.4 253,296 + GFF226 0.27 -0.7 253,297 - GFF226 0.27 -1.1 253,481 - GFF226 0.38 -0.4 253,612 + GFF226 0.46 -0.5 253,612 + GFF226 0.46 -0.1 253,612 + GFF226 0.46 -0.2 253,612 + GFF226 0.46 -0.5 253,612 + GFF226 0.46 -1.1 253,612 + GFF226 0.46 -1.7 253,613 - GFF226 0.46 -0.3 253,613 - GFF226 0.46 -1.1 253,718 - GFF226 0.53 -1.7 253,718 - GFF226 0.53 +0.1 253,718 - GFF226 0.53 +0.8
Or see this region's nucleotide sequence