Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1952 and GFF1953 overlap by 4 nucleotides GFF1953 and GFF1954 overlap by 4 nucleotides GFF1954 and GFF1955 overlap by 4 nucleotides
GFF1952 - Metallophosphoesterase, at 645,814 to 646,527
GFF1952
GFF1953 - hypothetical protein, at 646,524 to 647,171
GFF1953
GFF1954 - no description, at 647,168 to 648,433
GFF1954
GFF1955 - hypothetical protein, at 648,430 to 649,458
GFF1955
Position (kb) 647
648
649 Strain fitness (log2 ratio) -2
-1
0
1
2 at 646.249 kb on - strand, within GFF1952 at 646.249 kb on - strand, within GFF1952 at 646.414 kb on + strand, within GFF1952 at 646.414 kb on + strand, within GFF1952 at 646.414 kb on + strand, within GFF1952 at 646.415 kb on - strand, within GFF1952 at 646.415 kb on - strand, within GFF1952 at 646.415 kb on - strand, within GFF1952 at 646.415 kb on - strand, within GFF1952 at 646.415 kb on - strand, within GFF1952 at 646.520 kb on + strand at 646.520 kb on + strand at 646.536 kb on + strand at 646.536 kb on + strand at 646.536 kb on + strand at 646.536 kb on + strand at 646.536 kb on + strand at 646.536 kb on + strand at 646.537 kb on - strand at 646.568 kb on + strand at 646.622 kb on + strand, within GFF1953 at 646.622 kb on + strand, within GFF1953 at 646.622 kb on + strand, within GFF1953 at 646.628 kb on + strand, within GFF1953 at 646.628 kb on + strand, within GFF1953 at 646.629 kb on - strand, within GFF1953 at 646.705 kb on - strand, within GFF1953 at 646.705 kb on - strand, within GFF1953 at 646.711 kb on + strand, within GFF1953 at 646.770 kb on + strand, within GFF1953 at 646.771 kb on - strand, within GFF1953 at 646.771 kb on - strand, within GFF1953 at 646.854 kb on - strand, within GFF1953 at 647.038 kb on + strand, within GFF1953 at 647.099 kb on + strand, within GFF1953 at 647.099 kb on + strand, within GFF1953 at 647.099 kb on + strand, within GFF1953 at 647.099 kb on + strand, within GFF1953 at 647.100 kb on - strand, within GFF1953 at 647.100 kb on - strand, within GFF1953 at 647.100 kb on - strand, within GFF1953 at 647.285 kb on + strand at 647.286 kb on - strand at 647.392 kb on - strand, within GFF1954 at 647.488 kb on - strand, within GFF1954 at 647.865 kb on + strand, within GFF1954 at 647.890 kb on - strand, within GFF1954 at 647.890 kb on - strand, within GFF1954 at 647.955 kb on - strand, within GFF1954 at 648.511 kb on + strand at 648.511 kb on + strand at 648.511 kb on + strand at 648.511 kb on + strand at 648.512 kb on - strand at 648.512 kb on - strand at 648.512 kb on - strand at 648.512 kb on - strand at 648.533 kb on + strand, within GFF1955 at 648.562 kb on + strand, within GFF1955 at 648.562 kb on + strand, within GFF1955 at 648.562 kb on + strand, within GFF1955 at 648.562 kb on + strand, within GFF1955 at 648.562 kb on + strand, within GFF1955 at 648.562 kb on + strand, within GFF1955 at 648.602 kb on - strand, within GFF1955 at 648.693 kb on + strand, within GFF1955 at 648.694 kb on - strand, within GFF1955 at 648.724 kb on + strand, within GFF1955 at 648.724 kb on + strand, within GFF1955 at 648.724 kb on + strand, within GFF1955 at 648.724 kb on + strand, within GFF1955 at 648.724 kb on + strand, within GFF1955 at 648.725 kb on - strand, within GFF1955 at 648.725 kb on - strand, within GFF1955 at 648.725 kb on - strand, within GFF1955 at 648.725 kb on - strand, within GFF1955 at 648.726 kb on + strand, within GFF1955 at 648.726 kb on + strand, within GFF1955 at 648.726 kb on + strand, within GFF1955 at 648.727 kb on - strand, within GFF1955 at 648.727 kb on - strand, within GFF1955 at 648.727 kb on - strand, within GFF1955 at 648.727 kb on - strand, within GFF1955 at 648.895 kb on + strand, within GFF1955 at 648.895 kb on + strand, within GFF1955 at 648.896 kb on - strand, within GFF1955 at 648.896 kb on - strand, within GFF1955 at 648.896 kb on - strand, within GFF1955 at 648.896 kb on - strand, within GFF1955 at 648.899 kb on + strand, within GFF1955 at 648.899 kb on + strand, within GFF1955 at 648.900 kb on - strand, within GFF1955 at 648.900 kb on - strand, within GFF1955 at 648.900 kb on - strand, within GFF1955 at 649.009 kb on - strand, within GFF1955 at 649.107 kb on - strand, within GFF1955 at 649.274 kb on + strand, within GFF1955 at 649.309 kb on + strand, within GFF1955 at 649.365 kb on + strand at 649.365 kb on + strand at 649.365 kb on + strand at 649.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 646,249 - GFF1952 0.61 -0.1 646,249 - GFF1952 0.61 +1.1 646,414 + GFF1952 0.84 -1.0 646,414 + GFF1952 0.84 +0.5 646,414 + GFF1952 0.84 +1.2 646,415 - GFF1952 0.84 -0.4 646,415 - GFF1952 0.84 -0.3 646,415 - GFF1952 0.84 +0.4 646,415 - GFF1952 0.84 +1.1 646,415 - GFF1952 0.84 +1.5 646,520 + -1.5 646,520 + +0.5 646,536 + -0.5 646,536 + -0.0 646,536 + -1.2 646,536 + +0.8 646,536 + +1.6 646,536 + -0.2 646,537 - +0.6 646,568 + +0.7 646,622 + GFF1953 0.15 +0.1 646,622 + GFF1953 0.15 -1.5 646,622 + GFF1953 0.15 +1.4 646,628 + GFF1953 0.16 +0.5 646,628 + GFF1953 0.16 +0.8 646,629 - GFF1953 0.16 -1.7 646,705 - GFF1953 0.28 -0.3 646,705 - GFF1953 0.28 -0.6 646,711 + GFF1953 0.29 -0.2 646,770 + GFF1953 0.38 +0.4 646,771 - GFF1953 0.38 -0.1 646,771 - GFF1953 0.38 -0.9 646,854 - GFF1953 0.51 +1.8 647,038 + GFF1953 0.79 -0.7 647,099 + GFF1953 0.89 +0.3 647,099 + GFF1953 0.89 +0.3 647,099 + GFF1953 0.89 +0.3 647,099 + GFF1953 0.89 -0.5 647,100 - GFF1953 0.89 +0.3 647,100 - GFF1953 0.89 +0.1 647,100 - GFF1953 0.89 +0.3 647,285 + -1.5 647,286 - -0.0 647,392 - GFF1954 0.18 +0.8 647,488 - GFF1954 0.25 -0.9 647,865 + GFF1954 0.55 -0.5 647,890 - GFF1954 0.57 -0.7 647,890 - GFF1954 0.57 -0.0 647,955 - GFF1954 0.62 +0.8 648,511 + -1.7 648,511 + +0.7 648,511 + -0.7 648,511 + +0.3 648,512 - -0.7 648,512 - -0.3 648,512 - +0.1 648,512 - +0.3 648,533 + GFF1955 0.10 -0.3 648,562 + GFF1955 0.13 -0.5 648,562 + GFF1955 0.13 +0.2 648,562 + GFF1955 0.13 -0.2 648,562 + GFF1955 0.13 +1.3 648,562 + GFF1955 0.13 -0.4 648,562 + GFF1955 0.13 -0.9 648,602 - GFF1955 0.17 +1.1 648,693 + GFF1955 0.26 -0.9 648,694 - GFF1955 0.26 +1.3 648,724 + GFF1955 0.29 -1.1 648,724 + GFF1955 0.29 -0.1 648,724 + GFF1955 0.29 -0.5 648,724 + GFF1955 0.29 -0.5 648,724 + GFF1955 0.29 -0.2 648,725 - GFF1955 0.29 +0.0 648,725 - GFF1955 0.29 -0.2 648,725 - GFF1955 0.29 -0.2 648,725 - GFF1955 0.29 +0.5 648,726 + GFF1955 0.29 -0.7 648,726 + GFF1955 0.29 -0.2 648,726 + GFF1955 0.29 +0.2 648,727 - GFF1955 0.29 +0.1 648,727 - GFF1955 0.29 +0.2 648,727 - GFF1955 0.29 +1.8 648,727 - GFF1955 0.29 -0.8 648,895 + GFF1955 0.45 -2.0 648,895 + GFF1955 0.45 -1.3 648,896 - GFF1955 0.45 -0.5 648,896 - GFF1955 0.45 -0.4 648,896 - GFF1955 0.45 -0.5 648,896 - GFF1955 0.45 +0.5 648,899 + GFF1955 0.46 -0.6 648,899 + GFF1955 0.46 +0.8 648,900 - GFF1955 0.46 -0.8 648,900 - GFF1955 0.46 -0.2 648,900 - GFF1955 0.46 -0.4 649,009 - GFF1955 0.56 -1.5 649,107 - GFF1955 0.66 -0.5 649,274 + GFF1955 0.82 +0.0 649,309 + GFF1955 0.85 -0.3 649,365 + -1.2 649,365 + -1.1 649,365 + -0.7 649,375 - -1.8
Or see this region's nucleotide sequence