Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF191 and GFF192 are separated by 117 nucleotides GFF192 and GFF193 overlap by 4 nucleotides GFF193 and GFF194 are separated by 35 nucleotides
GFF191 - (Y14336) putative extracellular protein containing predicted 35aa signal peptide, at 220,808 to 221,308
GFF191
GFF192 - Nitrogen regulation protein NR(I), GlnG (=NtrC), at 221,426 to 222,067
GFF192
GFF193 - Two-component sensor PilS, at 222,064 to 223,569
GFF193
GFF194 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 223,605 to 224,699
GFF194
Position (kb) 222
223
224 Strain fitness (log2 ratio) -2
-1
0
1
2 at 221.145 kb on + strand, within GFF191 at 221.145 kb on + strand, within GFF191 at 221.146 kb on - strand, within GFF191 at 221.146 kb on - strand, within GFF191 at 221.146 kb on - strand, within GFF191 at 221.146 kb on - strand, within GFF191 at 221.370 kb on + strand at 221.371 kb on - strand at 221.399 kb on + strand at 221.400 kb on - strand at 221.421 kb on + strand at 221.422 kb on - strand at 221.427 kb on + strand at 221.428 kb on - strand at 223.127 kb on + strand, within GFF193 at 223.127 kb on + strand, within GFF193 at 223.127 kb on + strand, within GFF193 at 223.127 kb on + strand, within GFF193 at 223.128 kb on - strand, within GFF193 at 223.128 kb on - strand, within GFF193 at 223.128 kb on - strand, within GFF193 at 223.128 kb on - strand, within GFF193 at 223.138 kb on + strand, within GFF193 at 223.139 kb on - strand, within GFF193 at 223.160 kb on + strand, within GFF193 at 223.403 kb on + strand, within GFF193 at 223.404 kb on - strand, within GFF193 at 223.404 kb on - strand, within GFF193 at 223.404 kb on - strand, within GFF193 at 223.642 kb on + strand at 223.642 kb on + strand at 223.642 kb on + strand at 223.643 kb on - strand at 223.643 kb on - strand at 223.774 kb on + strand, within GFF194 at 223.775 kb on - strand, within GFF194 at 223.775 kb on - strand, within GFF194 at 223.830 kb on + strand, within GFF194 at 223.831 kb on - strand, within GFF194 at 223.831 kb on - strand, within GFF194 at 223.831 kb on - strand, within GFF194 at 223.867 kb on - strand, within GFF194 at 223.954 kb on - strand, within GFF194 at 223.977 kb on + strand, within GFF194 at 223.977 kb on + strand, within GFF194 at 224.494 kb on - strand, within GFF194 at 224.514 kb on + strand, within GFF194 at 224.515 kb on - strand, within GFF194 at 224.515 kb on - strand, within GFF194
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 221,145 + GFF191 0.67 +0.7 221,145 + GFF191 0.67 -0.2 221,146 - GFF191 0.67 +0.3 221,146 - GFF191 0.67 +0.4 221,146 - GFF191 0.67 +0.4 221,146 - GFF191 0.67 -0.3 221,370 + -1.6 221,371 - -1.8 221,399 + +2.0 221,400 - +1.1 221,421 + -0.2 221,422 - -0.1 221,427 + +0.4 221,428 - +0.6 223,127 + GFF193 0.71 -1.6 223,127 + GFF193 0.71 +0.4 223,127 + GFF193 0.71 -0.5 223,127 + GFF193 0.71 +0.3 223,128 - GFF193 0.71 -0.9 223,128 - GFF193 0.71 -0.8 223,128 - GFF193 0.71 -1.0 223,128 - GFF193 0.71 +0.4 223,138 + GFF193 0.71 -0.2 223,139 - GFF193 0.71 -1.1 223,160 + GFF193 0.73 -1.8 223,403 + GFF193 0.89 +0.8 223,404 - GFF193 0.89 -0.1 223,404 - GFF193 0.89 -0.3 223,404 - GFF193 0.89 -0.4 223,642 + -0.5 223,642 + -0.3 223,642 + -0.0 223,643 - -1.4 223,643 - -1.1 223,774 + GFF194 0.15 -0.7 223,775 - GFF194 0.16 +0.3 223,775 - GFF194 0.16 -0.2 223,830 + GFF194 0.21 -1.5 223,831 - GFF194 0.21 +1.4 223,831 - GFF194 0.21 +1.7 223,831 - GFF194 0.21 -1.1 223,867 - GFF194 0.24 +0.1 223,954 - GFF194 0.32 +0.3 223,977 + GFF194 0.34 +0.9 223,977 + GFF194 0.34 -0.7 224,494 - GFF194 0.81 -0.6 224,514 + GFF194 0.83 -0.1 224,515 - GFF194 0.83 -0.4 224,515 - GFF194 0.83 -0.1
Or see this region's nucleotide sequence