Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1798 and GFF1799 are separated by 23 nucleotides GFF1799 and GFF1800 are separated by 13 nucleotides GFF1800 and GFF1801 are separated by 113 nucleotides
GFF1798 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 460,805 to 461,713
GFF1798
GFF1799 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 461,737 to 462,522
GFF1799
GFF1800 - Glyoxylate carboligase (EC 4.1.1.47), at 462,536 to 464,329
GFF1800
GFF1801 - Transcriptional regulator, LysR family, at 464,443 to 465,345
GFF1801
Position (kb)
462
463
464
465 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 461.801 kb on + strand at 461.802 kb on - strand at 461.862 kb on - strand, within GFF1799 at 461.984 kb on + strand, within GFF1799 at 461.984 kb on + strand, within GFF1799 at 461.985 kb on - strand, within GFF1799 at 461.985 kb on - strand, within GFF1799 at 461.985 kb on - strand, within GFF1799 at 461.985 kb on - strand, within GFF1799 at 461.985 kb on - strand, within GFF1799 at 461.985 kb on - strand, within GFF1799 at 461.993 kb on + strand, within GFF1799 at 461.993 kb on + strand, within GFF1799 at 462.411 kb on - strand, within GFF1799 at 462.411 kb on - strand, within GFF1799 at 462.422 kb on + strand, within GFF1799 at 462.422 kb on + strand, within GFF1799 at 462.423 kb on - strand, within GFF1799 at 462.538 kb on - strand at 462.798 kb on + strand, within GFF1800 at 462.799 kb on - strand, within GFF1800 at 462.862 kb on - strand, within GFF1800 at 462.906 kb on + strand, within GFF1800 at 462.906 kb on + strand, within GFF1800 at 462.907 kb on - strand, within GFF1800 at 462.990 kb on + strand, within GFF1800 at 462.990 kb on + strand, within GFF1800 at 463.110 kb on + strand, within GFF1800 at 463.110 kb on + strand, within GFF1800 at 463.110 kb on + strand, within GFF1800 at 463.111 kb on - strand, within GFF1800 at 463.111 kb on - strand, within GFF1800 at 463.164 kb on + strand, within GFF1800 at 463.164 kb on + strand, within GFF1800 at 463.164 kb on + strand, within GFF1800 at 463.165 kb on - strand, within GFF1800 at 463.165 kb on - strand, within GFF1800 at 463.165 kb on - strand, within GFF1800 at 463.165 kb on - strand, within GFF1800 at 463.203 kb on + strand, within GFF1800 at 463.455 kb on + strand, within GFF1800 at 463.456 kb on - strand, within GFF1800 at 463.456 kb on - strand, within GFF1800 at 463.779 kb on + strand, within GFF1800 at 463.780 kb on - strand, within GFF1800 at 464.001 kb on + strand, within GFF1800 at 464.001 kb on + strand, within GFF1800 at 464.148 kb on + strand, within GFF1800 at 464.148 kb on + strand, within GFF1800 at 464.149 kb on - strand, within GFF1800 at 464.229 kb on + strand at 464.229 kb on + strand at 464.360 kb on + strand at 464.360 kb on + strand at 464.360 kb on + strand at 464.422 kb on + strand at 464.635 kb on + strand, within GFF1801 at 464.635 kb on + strand, within GFF1801 at 464.638 kb on + strand, within GFF1801 at 464.638 kb on + strand, within GFF1801 at 464.639 kb on - strand, within GFF1801 at 464.639 kb on - strand, within GFF1801 at 464.639 kb on - strand, within GFF1801 at 464.639 kb on - strand, within GFF1801 at 464.782 kb on + strand, within GFF1801 at 464.782 kb on + strand, within GFF1801 at 464.782 kb on + strand, within GFF1801 at 464.782 kb on + strand, within GFF1801 at 464.782 kb on + strand, within GFF1801 at 464.783 kb on - strand, within GFF1801 at 464.783 kb on - strand, within GFF1801 at 464.783 kb on - strand, within GFF1801 at 464.783 kb on - strand, within GFF1801 at 464.783 kb on - strand, within GFF1801 at 464.954 kb on - strand, within GFF1801 at 465.169 kb on + strand, within GFF1801 at 465.170 kb on - strand, within GFF1801 at 465.170 kb on - strand, within GFF1801 at 465.170 kb on - strand, within GFF1801 at 465.170 kb on - strand, within GFF1801
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 461,801 + +0.0 461,802 - -0.6 461,862 - GFF1799 0.16 -0.6 461,984 + GFF1799 0.31 -0.1 461,984 + GFF1799 0.31 -0.3 461,985 - GFF1799 0.32 +0.5 461,985 - GFF1799 0.32 -2.3 461,985 - GFF1799 0.32 -0.2 461,985 - GFF1799 0.32 -1.2 461,985 - GFF1799 0.32 -0.4 461,985 - GFF1799 0.32 -0.4 461,993 + GFF1799 0.33 -1.0 461,993 + GFF1799 0.33 -0.4 462,411 - GFF1799 0.86 -0.5 462,411 - GFF1799 0.86 -1.5 462,422 + GFF1799 0.87 -0.0 462,422 + GFF1799 0.87 +0.1 462,423 - GFF1799 0.87 -0.1 462,538 - +0.5 462,798 + GFF1800 0.15 -0.3 462,799 - GFF1800 0.15 -0.9 462,862 - GFF1800 0.18 -0.1 462,906 + GFF1800 0.21 -1.3 462,906 + GFF1800 0.21 -0.2 462,907 - GFF1800 0.21 -0.8 462,990 + GFF1800 0.25 +0.3 462,990 + GFF1800 0.25 -0.4 463,110 + GFF1800 0.32 -1.6 463,110 + GFF1800 0.32 -0.6 463,110 + GFF1800 0.32 +1.8 463,111 - GFF1800 0.32 -0.6 463,111 - GFF1800 0.32 +0.1 463,164 + GFF1800 0.35 -0.7 463,164 + GFF1800 0.35 -0.1 463,164 + GFF1800 0.35 +0.1 463,165 - GFF1800 0.35 -0.7 463,165 - GFF1800 0.35 -0.7 463,165 - GFF1800 0.35 -1.1 463,165 - GFF1800 0.35 -0.7 463,203 + GFF1800 0.37 +0.2 463,455 + GFF1800 0.51 -0.8 463,456 - GFF1800 0.51 +2.4 463,456 - GFF1800 0.51 -0.3 463,779 + GFF1800 0.69 -0.8 463,780 - GFF1800 0.69 -0.6 464,001 + GFF1800 0.82 -0.2 464,001 + GFF1800 0.82 +0.4 464,148 + GFF1800 0.90 -2.0 464,148 + GFF1800 0.90 -0.4 464,149 - GFF1800 0.90 +0.4 464,229 + -0.6 464,229 + -0.9 464,360 + +0.3 464,360 + -1.4 464,360 + +1.8 464,422 + +0.3 464,635 + GFF1801 0.21 -0.4 464,635 + GFF1801 0.21 +0.5 464,638 + GFF1801 0.22 -1.0 464,638 + GFF1801 0.22 +0.2 464,639 - GFF1801 0.22 +0.8 464,639 - GFF1801 0.22 -0.2 464,639 - GFF1801 0.22 -0.0 464,639 - GFF1801 0.22 -0.4 464,782 + GFF1801 0.38 -0.8 464,782 + GFF1801 0.38 -1.9 464,782 + GFF1801 0.38 +0.3 464,782 + GFF1801 0.38 -0.4 464,782 + GFF1801 0.38 -1.0 464,783 - GFF1801 0.38 -2.0 464,783 - GFF1801 0.38 -1.3 464,783 - GFF1801 0.38 -0.8 464,783 - GFF1801 0.38 +0.2 464,783 - GFF1801 0.38 -0.3 464,954 - GFF1801 0.57 -0.5 465,169 + GFF1801 0.80 +0.4 465,170 - GFF1801 0.81 -0.3 465,170 - GFF1801 0.81 +0.7 465,170 - GFF1801 0.81 +0.4 465,170 - GFF1801 0.81 +1.4
Or see this region's nucleotide sequence