Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1737 and GFF1738 are separated by 4 nucleotides GFF1738 and GFF1739 are separated by 128 nucleotides GFF1739 and GFF1740 are separated by 9 nucleotides GFF1740 and GFF1741 are separated by 76 nucleotides
GFF1737 - Carbon starvation protein A, at 396,223 to 398,289
GFF1737
GFF1738 - Uncharacterized proteins YbdD and YjiX, at 398,294 to 398,500
GFF1738
GFF1739 - Glutamate carboxypeptidase (EC 3.4.17.11), at 398,629 to 399,906
GFF1739
GFF1740 - Transcriptional regulator, LysR family, at 399,916 to 400,827
GFF1740
GFF1741 - UPF0324 inner membrane protein YeiH, at 400,904 to 401,956
GFF1741
Position (kb) 398
399
400 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 397.668 kb on - strand, within GFF1737 at 397.807 kb on + strand, within GFF1737 at 397.808 kb on - strand, within GFF1737 at 397.940 kb on - strand, within GFF1737 at 398.057 kb on - strand, within GFF1737 at 398.194 kb on + strand at 398.194 kb on + strand at 398.195 kb on - strand at 398.324 kb on + strand, within GFF1738 at 398.379 kb on - strand, within GFF1738 at 398.388 kb on - strand, within GFF1738 at 398.435 kb on + strand, within GFF1738 at 398.498 kb on + strand at 398.499 kb on - strand at 398.499 kb on - strand at 398.559 kb on + strand at 398.559 kb on + strand at 398.559 kb on + strand at 398.560 kb on - strand at 398.560 kb on - strand at 398.560 kb on - strand at 398.785 kb on + strand, within GFF1739 at 398.786 kb on - strand, within GFF1739 at 398.786 kb on - strand, within GFF1739 at 398.786 kb on - strand, within GFF1739 at 399.049 kb on + strand, within GFF1739 at 399.049 kb on + strand, within GFF1739 at 399.050 kb on - strand, within GFF1739 at 399.050 kb on - strand, within GFF1739 at 399.050 kb on - strand, within GFF1739 at 399.050 kb on - strand, within GFF1739 at 399.119 kb on - strand, within GFF1739 at 399.119 kb on - strand, within GFF1739 at 399.157 kb on + strand, within GFF1739 at 399.694 kb on + strand, within GFF1739 at 399.695 kb on - strand, within GFF1739 at 399.695 kb on - strand, within GFF1739 at 399.862 kb on + strand at 399.862 kb on + strand at 399.863 kb on - strand at 400.476 kb on - strand, within GFF1740 at 400.500 kb on - strand, within GFF1740 at 400.514 kb on + strand, within GFF1740 at 400.515 kb on - strand, within GFF1740 at 400.891 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 397,668 - GFF1737 0.70 -0.2 397,807 + GFF1737 0.77 -1.7 397,808 - GFF1737 0.77 +0.3 397,940 - GFF1737 0.83 -0.3 398,057 - GFF1737 0.89 -0.3 398,194 + +0.3 398,194 + -0.5 398,195 - +1.0 398,324 + GFF1738 0.14 -0.1 398,379 - GFF1738 0.41 -0.7 398,388 - GFF1738 0.45 +0.6 398,435 + GFF1738 0.68 -0.6 398,498 + -2.9 398,499 - -1.2 398,499 - -0.5 398,559 + +0.3 398,559 + -0.0 398,559 + -1.2 398,560 - +0.8 398,560 - -1.7 398,560 - -0.3 398,785 + GFF1739 0.12 -0.1 398,786 - GFF1739 0.12 +0.5 398,786 - GFF1739 0.12 -0.9 398,786 - GFF1739 0.12 -0.9 399,049 + GFF1739 0.33 -1.2 399,049 + GFF1739 0.33 -0.0 399,050 - GFF1739 0.33 +3.4 399,050 - GFF1739 0.33 +0.5 399,050 - GFF1739 0.33 +1.8 399,050 - GFF1739 0.33 +0.2 399,119 - GFF1739 0.38 -1.6 399,119 - GFF1739 0.38 -0.0 399,157 + GFF1739 0.41 +0.2 399,694 + GFF1739 0.83 +0.5 399,695 - GFF1739 0.83 +1.0 399,695 - GFF1739 0.83 +0.0 399,862 + +1.0 399,862 + -0.3 399,863 - -0.6 400,476 - GFF1740 0.61 +1.4 400,500 - GFF1740 0.64 +0.0 400,514 + GFF1740 0.66 +1.4 400,515 - GFF1740 0.66 -0.5 400,891 + +0.2
Or see this region's nucleotide sequence