Strain Fitness in Variovorax sp. SCN45 around GFF1621

Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1620 and GFF1621 are separated by 204 nucleotidesGFF1621 and GFF1622 are separated by 23 nucleotides GFF1620 - Large exoproteins involved in heme utilization or adhesion, at 218,085 to 220,358 GFF1620 GFF1621 - Phosphonoacetaldehyde dehydrogenase (NAD+), at 220,563 to 222,011 GFF1621 GFF1622 - Phosphonoacetate hydrolase (EC 3.11.1.2), at 222,035 to 223,261 GFF1622 Position (kb) 220 221 222 223Strain fitness (log2 ratio) -1 0 1at 219.967 kb on + strand, within GFF1620at 219.968 kb on - strand, within GFF1620at 220.044 kb on - strand, within GFF1620at 220.744 kb on + strand, within GFF1621at 220.744 kb on + strand, within GFF1621at 220.784 kb on - strand, within GFF1621at 221.164 kb on + strand, within GFF1621at 221.164 kb on + strand, within GFF1621at 221.263 kb on + strand, within GFF1621at 221.585 kb on - strand, within GFF1621at 221.914 kb on - strandat 223.003 kb on - strand, within GFF1622

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
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219,967 + GFF1620 0.83 +0.0
219,968 - GFF1620 0.83 +0.6
220,044 - GFF1620 0.86 +0.9
220,744 + GFF1621 0.12 -0.2
220,744 + GFF1621 0.12 +0.1
220,784 - GFF1621 0.15 +0.5
221,164 + GFF1621 0.41 -0.1
221,164 + GFF1621 0.41 -1.6
221,263 + GFF1621 0.48 -0.1
221,585 - GFF1621 0.71 -0.3
221,914 - +0.3
223,003 - GFF1622 0.79 +1.1

Or see this region's nucleotide sequence