Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF132 and GFF133 are separated by 35 nucleotides GFF133 and GFF134 overlap by 4 nucleotides GFF134 and GFF135 are separated by 142 nucleotides GFF135 and GFF136 are separated by 10 nucleotides
GFF132 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 160,488 to 161,459
GFF132
GFF133 - Ferredoxin, at 161,495 to 161,824
GFF133
GFF134 - putative oxidoreductase (AsfA-like), putative L-aspartate oxidase( EC:1.4.3.16 ), at 161,821 to 163,443
GFF134
GFF135 - no description, at 163,586 to 164,020
GFF135
GFF136 - Uncharacterized MFS-type transporter, at 164,031 to 165,458
GFF136
Position (kb) 161
162
163
164 Strain fitness (log2 ratio) -2
-1
0
1 at 160.996 kb on + strand, within GFF132 at 160.996 kb on + strand, within GFF132 at 160.997 kb on - strand, within GFF132 at 161.008 kb on + strand, within GFF132 at 161.008 kb on + strand, within GFF132 at 161.008 kb on + strand, within GFF132 at 161.009 kb on - strand, within GFF132 at 161.009 kb on - strand, within GFF132 at 161.170 kb on + strand, within GFF132 at 161.170 kb on + strand, within GFF132 at 161.580 kb on + strand, within GFF133 at 161.581 kb on - strand, within GFF133 at 161.655 kb on + strand, within GFF133 at 161.676 kb on + strand, within GFF133 at 161.676 kb on + strand, within GFF133 at 161.972 kb on + strand at 161.973 kb on - strand at 162.389 kb on + strand, within GFF134 at 162.680 kb on + strand, within GFF134 at 162.681 kb on - strand, within GFF134 at 163.031 kb on + strand, within GFF134 at 163.032 kb on - strand, within GFF134 at 163.032 kb on - strand, within GFF134 at 163.479 kb on + strand at 163.480 kb on - strand at 163.524 kb on + strand at 163.697 kb on + strand, within GFF135 at 163.697 kb on + strand, within GFF135 at 163.697 kb on + strand, within GFF135 at 163.697 kb on + strand, within GFF135 at 163.697 kb on + strand, within GFF135 at 163.697 kb on + strand, within GFF135 at 163.697 kb on + strand, within GFF135 at 163.698 kb on - strand, within GFF135 at 163.698 kb on - strand, within GFF135 at 163.754 kb on + strand, within GFF135 at 163.754 kb on + strand, within GFF135 at 163.755 kb on - strand, within GFF135 at 163.755 kb on - strand, within GFF135 at 163.919 kb on + strand, within GFF135 at 163.919 kb on + strand, within GFF135 at 163.919 kb on + strand, within GFF135 at 163.934 kb on + strand, within GFF135 at 163.943 kb on + strand, within GFF135 at 163.976 kb on + strand, within GFF135 at 163.976 kb on + strand, within GFF135 at 163.976 kb on + strand, within GFF135 at 163.977 kb on - strand at 164.032 kb on + strand at 164.032 kb on + strand at 164.033 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 160,996 + GFF132 0.52 -0.8 160,996 + GFF132 0.52 +1.2 160,997 - GFF132 0.52 +0.1 161,008 + GFF132 0.53 -1.0 161,008 + GFF132 0.53 -0.0 161,008 + GFF132 0.53 +0.6 161,009 - GFF132 0.54 -0.0 161,009 - GFF132 0.54 -0.2 161,170 + GFF132 0.70 +1.0 161,170 + GFF132 0.70 +0.0 161,580 + GFF133 0.26 +0.2 161,581 - GFF133 0.26 -0.2 161,655 + GFF133 0.48 -1.0 161,676 + GFF133 0.55 -1.0 161,676 + GFF133 0.55 +0.9 161,972 + -0.3 161,973 - +0.1 162,389 + GFF134 0.35 -0.1 162,680 + GFF134 0.53 -0.9 162,681 - GFF134 0.53 -0.5 163,031 + GFF134 0.75 -0.5 163,032 - GFF134 0.75 +0.4 163,032 - GFF134 0.75 -2.1 163,479 + +0.1 163,480 - -0.1 163,524 + -0.5 163,697 + GFF135 0.26 -0.5 163,697 + GFF135 0.26 +1.5 163,697 + GFF135 0.26 +1.1 163,697 + GFF135 0.26 -0.9 163,697 + GFF135 0.26 -0.1 163,697 + GFF135 0.26 -0.0 163,697 + GFF135 0.26 +1.2 163,698 - GFF135 0.26 +0.5 163,698 - GFF135 0.26 -0.4 163,754 + GFF135 0.39 +0.9 163,754 + GFF135 0.39 +1.5 163,755 - GFF135 0.39 +0.5 163,755 - GFF135 0.39 +0.5 163,919 + GFF135 0.77 -0.4 163,919 + GFF135 0.77 +0.6 163,919 + GFF135 0.77 -1.8 163,934 + GFF135 0.80 +0.8 163,943 + GFF135 0.82 -0.2 163,976 + GFF135 0.90 -2.3 163,976 + GFF135 0.90 +0.1 163,976 + GFF135 0.90 -1.4 163,977 - +0.2 164,032 + +0.4 164,032 + -1.1 164,033 - -0.9
Or see this region's nucleotide sequence