Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1304 and GFF1305 overlap by 4 nucleotides GFF1305 and GFF1306 are separated by 38 nucleotides GFF1306 and GFF1307 are separated by 106 nucleotides
GFF1304 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 634,782 to 635,789
GFF1304
GFF1305 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 635,786 to 636,841
GFF1305
GFF1306 - no description, at 636,880 to 637,005
GFF1306
GFF1307 - Homoserine dehydrogenase (EC 1.1.1.3), at 637,112 to 638,476
GFF1307
Position (kb) 635
636
637 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 635.145 kb on + strand, within GFF1304 at 635.145 kb on + strand, within GFF1304 at 635.145 kb on + strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.321 kb on + strand, within GFF1304 at 635.321 kb on + strand, within GFF1304 at 635.322 kb on - strand, within GFF1304 at 635.322 kb on - strand, within GFF1304 at 635.349 kb on - strand, within GFF1304 at 635.349 kb on - strand, within GFF1304 at 635.388 kb on + strand, within GFF1304 at 635.388 kb on + strand, within GFF1304 at 635.388 kb on + strand, within GFF1304 at 635.388 kb on + strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.416 kb on + strand, within GFF1304 at 635.419 kb on - strand, within GFF1304 at 635.419 kb on - strand, within GFF1304 at 635.712 kb on + strand at 635.712 kb on + strand at 635.712 kb on + strand at 635.712 kb on + strand at 635.712 kb on + strand at 635.713 kb on - strand at 635.713 kb on - strand at 635.713 kb on - strand at 635.754 kb on + strand at 635.754 kb on + strand at 635.754 kb on + strand at 636.335 kb on + strand, within GFF1305 at 636.335 kb on + strand, within GFF1305 at 636.336 kb on - strand, within GFF1305 at 636.336 kb on - strand, within GFF1305 at 636.336 kb on - strand, within GFF1305 at 636.476 kb on + strand, within GFF1305 at 636.476 kb on + strand, within GFF1305 at 636.476 kb on + strand, within GFF1305 at 636.477 kb on - strand, within GFF1305 at 636.737 kb on + strand at 636.737 kb on + strand at 636.737 kb on + strand at 636.738 kb on - strand at 636.738 kb on - strand at 636.749 kb on + strand at 636.750 kb on - strand at 636.770 kb on + strand at 636.770 kb on + strand at 636.800 kb on + strand at 636.801 kb on - strand at 636.864 kb on + strand at 636.987 kb on + strand, within GFF1306 at 636.987 kb on + strand, within GFF1306 at 636.987 kb on + strand, within GFF1306 at 636.988 kb on - strand, within GFF1306 at 636.988 kb on - strand, within GFF1306 at 637.041 kb on + strand at 637.041 kb on + strand at 637.041 kb on + strand at 637.041 kb on + strand at 637.041 kb on + strand at 637.041 kb on + strand at 637.041 kb on + strand at 637.042 kb on - strand at 637.042 kb on - strand at 637.042 kb on - strand at 637.042 kb on - strand at 637.042 kb on - strand at 637.042 kb on - strand at 637.115 kb on + strand at 637.115 kb on + strand at 637.115 kb on + strand at 637.115 kb on + strand at 637.115 kb on + strand at 637.115 kb on + strand at 637.115 kb on + strand at 637.116 kb on - strand at 637.116 kb on - strand at 637.116 kb on - strand at 637.116 kb on - strand at 637.116 kb on - strand at 637.174 kb on + strand at 637.174 kb on + strand at 637.175 kb on - strand at 637.325 kb on - strand, within GFF1307 at 637.325 kb on - strand, within GFF1307 at 637.551 kb on - strand, within GFF1307 at 637.551 kb on - strand, within GFF1307 at 637.551 kb on - strand, within GFF1307
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 635,145 + GFF1304 0.36 +0.1 635,145 + GFF1304 0.36 -0.6 635,145 + GFF1304 0.36 -3.1 635,146 - GFF1304 0.36 -0.7 635,146 - GFF1304 0.36 -1.0 635,146 - GFF1304 0.36 -0.8 635,146 - GFF1304 0.36 -0.5 635,146 - GFF1304 0.36 +0.1 635,146 - GFF1304 0.36 +0.5 635,321 + GFF1304 0.53 -0.0 635,321 + GFF1304 0.53 -0.6 635,322 - GFF1304 0.54 +0.3 635,322 - GFF1304 0.54 +0.7 635,349 - GFF1304 0.56 +1.1 635,349 - GFF1304 0.56 -0.8 635,388 + GFF1304 0.60 +0.9 635,388 + GFF1304 0.60 -0.3 635,388 + GFF1304 0.60 +0.1 635,388 + GFF1304 0.60 +0.2 635,389 - GFF1304 0.60 -0.5 635,389 - GFF1304 0.60 +1.1 635,389 - GFF1304 0.60 +0.6 635,389 - GFF1304 0.60 +0.4 635,389 - GFF1304 0.60 +0.2 635,416 + GFF1304 0.63 -0.7 635,419 - GFF1304 0.63 -0.2 635,419 - GFF1304 0.63 +0.3 635,712 + -0.6 635,712 + +0.3 635,712 + -2.6 635,712 + +0.6 635,712 + -0.6 635,713 - -0.5 635,713 - +0.1 635,713 - -1.3 635,754 + -0.0 635,754 + -0.8 635,754 + +0.5 636,335 + GFF1305 0.52 -1.6 636,335 + GFF1305 0.52 +0.8 636,336 - GFF1305 0.52 -2.3 636,336 - GFF1305 0.52 -2.2 636,336 - GFF1305 0.52 +0.1 636,476 + GFF1305 0.65 -1.1 636,476 + GFF1305 0.65 +0.3 636,476 + GFF1305 0.65 -1.9 636,477 - GFF1305 0.65 +0.9 636,737 + -0.1 636,737 + -1.1 636,737 + -0.5 636,738 - -0.9 636,738 - -0.8 636,749 + -1.3 636,750 - -0.9 636,770 + -0.6 636,770 + +0.3 636,800 + +0.3 636,801 - -0.7 636,864 + +0.7 636,987 + GFF1306 0.85 +0.3 636,987 + GFF1306 0.85 -1.5 636,987 + GFF1306 0.85 -0.6 636,988 - GFF1306 0.86 -1.3 636,988 - GFF1306 0.86 -0.2 637,041 + -0.8 637,041 + +0.3 637,041 + -0.9 637,041 + +0.1 637,041 + +1.0 637,041 + -0.1 637,041 + +0.0 637,042 - +0.3 637,042 - +0.1 637,042 - +0.1 637,042 - +1.1 637,042 - -1.2 637,042 - -1.9 637,115 + -0.9 637,115 + +0.7 637,115 + -1.4 637,115 + +0.2 637,115 + -1.4 637,115 + +1.5 637,115 + -1.1 637,116 - -0.3 637,116 - +0.1 637,116 - -1.1 637,116 - -0.2 637,116 - -0.2 637,174 + +0.4 637,174 + -1.6 637,175 - +0.7 637,325 - GFF1307 0.16 -0.9 637,325 - GFF1307 0.16 -0.3 637,551 - GFF1307 0.32 +0.6 637,551 - GFF1307 0.32 -1.9 637,551 - GFF1307 0.32 -3.0
Or see this region's nucleotide sequence