Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1272 and GFF1273 are separated by 719 nucleotides GFF1273 and GFF1274 are separated by 141 nucleotides GFF1274 and GFF1275 are separated by 4 nucleotides
GFF1272 - no description, at 602,259 to 603,113
GFF1272
GFF1273 - no description, at 603,833 to 604,066
GFF1273
GFF1274 - Glutamate racemase (EC 5.1.1.3), at 604,208 to 604,903
GFF1274
GFF1275 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 604,908 to 605,840
GFF1275
Position (kb) 603
604
605 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 602.882 kb on - strand, within GFF1272 at 602.923 kb on - strand, within GFF1272 at 602.934 kb on + strand, within GFF1272 at 602.935 kb on - strand, within GFF1272 at 602.936 kb on + strand, within GFF1272 at 602.936 kb on + strand, within GFF1272 at 602.960 kb on + strand, within GFF1272 at 602.961 kb on - strand, within GFF1272 at 603.009 kb on - strand, within GFF1272 at 603.037 kb on + strand at 603.038 kb on - strand at 603.040 kb on + strand at 603.043 kb on + strand at 603.184 kb on - strand at 603.186 kb on - strand at 603.320 kb on + strand at 603.321 kb on - strand at 603.321 kb on - strand at 603.366 kb on + strand at 603.376 kb on - strand at 603.377 kb on - strand at 603.410 kb on + strand at 603.452 kb on + strand at 603.478 kb on - strand at 603.478 kb on - strand at 603.482 kb on + strand at 603.482 kb on + strand at 603.482 kb on + strand at 603.482 kb on + strand at 603.482 kb on + strand at 603.483 kb on - strand at 603.483 kb on - strand at 603.483 kb on - strand at 603.483 kb on - strand at 603.508 kb on + strand at 603.645 kb on + strand at 603.645 kb on + strand at 603.645 kb on + strand at 603.645 kb on + strand at 603.645 kb on + strand at 603.645 kb on + strand at 603.645 kb on + strand at 603.645 kb on + strand at 603.645 kb on + strand at 603.645 kb on + strand at 603.645 kb on + strand at 603.645 kb on + strand at 603.645 kb on + strand at 603.645 kb on + strand at 603.646 kb on - strand at 603.646 kb on - strand at 603.646 kb on - strand at 603.646 kb on - strand at 603.646 kb on - strand at 603.646 kb on - strand at 603.646 kb on - strand at 603.646 kb on - strand at 603.646 kb on - strand at 603.646 kb on - strand at 603.663 kb on + strand at 603.663 kb on + strand at 603.663 kb on + strand at 603.663 kb on + strand at 603.663 kb on + strand at 603.663 kb on + strand at 603.664 kb on - strand at 603.664 kb on - strand at 603.664 kb on - strand at 603.699 kb on + strand at 603.699 kb on + strand at 603.699 kb on + strand at 603.700 kb on - strand at 603.700 kb on - strand at 603.700 kb on - strand at 603.750 kb on - strand at 603.750 kb on - strand at 603.750 kb on - strand at 603.750 kb on - strand at 603.750 kb on - strand at 603.815 kb on + strand at 603.815 kb on + strand at 603.815 kb on + strand at 603.815 kb on + strand at 603.816 kb on - strand at 603.816 kb on - strand at 603.816 kb on - strand at 603.816 kb on - strand at 603.816 kb on - strand at 603.875 kb on + strand, within GFF1273 at 603.875 kb on + strand, within GFF1273 at 603.876 kb on - strand, within GFF1273 at 603.876 kb on - strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.955 kb on - strand, within GFF1273 at 603.955 kb on - strand, within GFF1273 at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.070 kb on - strand at 604.070 kb on - strand at 604.070 kb on - strand at 604.070 kb on - strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.154 kb on + strand at 604.154 kb on + strand at 604.154 kb on + strand at 604.154 kb on + strand at 604.154 kb on + strand at 604.154 kb on + strand at 604.154 kb on + strand at 604.155 kb on - strand at 604.155 kb on - strand at 604.155 kb on - strand at 604.155 kb on - strand at 604.155 kb on - strand at 604.155 kb on - strand at 604.209 kb on + strand at 604.209 kb on + strand at 604.210 kb on - strand at 604.301 kb on + strand, within GFF1274 at 604.301 kb on + strand, within GFF1274 at 604.301 kb on + strand, within GFF1274 at 604.301 kb on + strand, within GFF1274 at 604.301 kb on + strand, within GFF1274 at 604.301 kb on + strand, within GFF1274 at 604.302 kb on - strand, within GFF1274 at 604.419 kb on + strand, within GFF1274 at 604.728 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.939 kb on + strand at 604.939 kb on + strand at 604.939 kb on + strand at 604.939 kb on + strand at 604.939 kb on + strand at 604.939 kb on + strand at 604.939 kb on + strand at 604.939 kb on + strand at 604.940 kb on - strand at 604.940 kb on - strand at 604.957 kb on + strand at 604.957 kb on + strand at 604.957 kb on + strand at 604.957 kb on + strand at 604.957 kb on + strand at 604.957 kb on + strand at 604.957 kb on + strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 602,882 - GFF1272 0.73 +0.1 602,923 - GFF1272 0.78 +0.5 602,934 + GFF1272 0.79 -0.1 602,935 - GFF1272 0.79 -0.4 602,936 + GFF1272 0.79 +2.1 602,936 + GFF1272 0.79 -2.1 602,960 + GFF1272 0.82 -0.3 602,961 - GFF1272 0.82 -0.5 603,009 - GFF1272 0.88 -1.5 603,037 + -0.1 603,038 - -1.2 603,040 + +0.1 603,043 + -1.3 603,184 - -0.1 603,186 - -0.3 603,320 + +0.7 603,321 - +0.3 603,321 - +0.7 603,366 + +1.7 603,376 - -1.8 603,377 - -0.9 603,410 + -0.4 603,452 + -1.9 603,478 - -0.7 603,478 - +0.2 603,482 + -1.6 603,482 + +0.9 603,482 + +1.1 603,482 + +0.1 603,482 + +4.0 603,483 - -2.1 603,483 - -0.5 603,483 - +0.8 603,483 - -0.4 603,508 + -0.4 603,645 + +0.3 603,645 + -1.2 603,645 + +0.2 603,645 + +0.3 603,645 + -0.6 603,645 + +0.6 603,645 + +0.4 603,645 + +0.1 603,645 + +0.9 603,645 + -0.4 603,645 + +0.3 603,645 + +0.3 603,645 + +0.3 603,645 + +0.1 603,646 - -0.5 603,646 - -0.5 603,646 - -0.9 603,646 - -0.1 603,646 - -1.4 603,646 - +0.4 603,646 - +0.5 603,646 - -0.8 603,646 - +0.0 603,646 - +0.4 603,663 + -0.1 603,663 + +1.3 603,663 + -1.1 603,663 + -1.1 603,663 + -1.9 603,663 + -1.1 603,664 - -0.3 603,664 - -0.8 603,664 - -0.5 603,699 + -0.8 603,699 + +0.3 603,699 + +1.0 603,700 - -0.1 603,700 - +0.4 603,700 - +0.0 603,750 - +0.5 603,750 - +0.3 603,750 - -1.1 603,750 - -1.1 603,750 - -0.6 603,815 + -0.5 603,815 + -1.2 603,815 + -1.9 603,815 + -0.3 603,816 - -0.1 603,816 - -0.5 603,816 - -0.0 603,816 - -0.9 603,816 - -1.8 603,875 + GFF1273 0.18 -0.5 603,875 + GFF1273 0.18 -0.3 603,876 - GFF1273 0.18 -0.1 603,876 - GFF1273 0.18 -0.3 603,954 + GFF1273 0.52 -0.2 603,954 + GFF1273 0.52 -0.3 603,954 + GFF1273 0.52 +1.0 603,954 + GFF1273 0.52 +0.3 603,954 + GFF1273 0.52 -0.8 603,954 + GFF1273 0.52 +0.1 603,954 + GFF1273 0.52 +0.3 603,954 + GFF1273 0.52 +1.5 603,955 - GFF1273 0.52 +0.9 603,955 - GFF1273 0.52 +0.2 604,069 + +0.2 604,069 + -0.5 604,069 + -1.1 604,069 + +0.5 604,069 + +0.5 604,069 + -0.1 604,069 + +0.4 604,069 + +1.3 604,069 + -0.8 604,069 + -0.2 604,070 - +0.9 604,070 - -0.5 604,070 - -0.3 604,070 - +1.2 604,147 + +0.8 604,147 + -1.8 604,147 + +0.4 604,147 + -0.9 604,147 + -1.6 604,147 + +0.0 604,147 + -1.5 604,147 + +0.1 604,147 + -0.5 604,147 + +1.2 604,147 + +1.5 604,147 + -0.5 604,148 - -0.1 604,148 - +0.3 604,148 - +0.3 604,148 - -0.1 604,148 - -0.5 604,148 - +0.8 604,148 - -0.7 604,148 - -1.3 604,148 - -1.3 604,154 + -1.2 604,154 + +0.5 604,154 + -0.2 604,154 + -0.6 604,154 + -1.6 604,154 + -0.2 604,154 + -0.2 604,155 - +0.8 604,155 - -0.1 604,155 - +0.2 604,155 - +1.9 604,155 - +0.2 604,155 - +0.2 604,209 + +0.7 604,209 + -0.8 604,210 - -0.4 604,301 + GFF1274 0.13 -0.3 604,301 + GFF1274 0.13 -1.0 604,301 + GFF1274 0.13 +1.3 604,301 + GFF1274 0.13 -0.7 604,301 + GFF1274 0.13 -0.6 604,301 + GFF1274 0.13 -0.8 604,302 - GFF1274 0.14 +2.1 604,419 + GFF1274 0.30 -1.0 604,728 + GFF1274 0.75 +1.5 604,729 + GFF1274 0.75 -0.7 604,729 + GFF1274 0.75 -0.9 604,729 + GFF1274 0.75 -0.8 604,729 + GFF1274 0.75 -1.2 604,729 + GFF1274 0.75 +0.9 604,729 + GFF1274 0.75 +1.2 604,729 + GFF1274 0.75 +0.0 604,729 + GFF1274 0.75 -0.5 604,729 + GFF1274 0.75 +0.2 604,730 - GFF1274 0.75 -0.7 604,730 - GFF1274 0.75 +0.3 604,730 - GFF1274 0.75 -1.1 604,730 - GFF1274 0.75 -0.4 604,730 - GFF1274 0.75 +0.2 604,730 - GFF1274 0.75 -0.3 604,730 - GFF1274 0.75 -1.7 604,939 + -0.6 604,939 + +1.8 604,939 + +0.7 604,939 + -1.1 604,939 + +0.1 604,939 + -0.0 604,939 + +0.0 604,939 + +0.9 604,940 - -0.5 604,940 - +1.5 604,957 + +0.6 604,957 + -0.2 604,957 + -1.1 604,957 + -0.2 604,957 + -1.3 604,957 + -1.0 604,957 + +1.3 604,958 - +0.5 604,958 - -0.3 604,958 - -0.2 604,958 - -0.4 604,958 - -0.4 604,958 - +0.9 604,958 - -1.2 604,958 - -0.4 604,958 - +0.1
Or see this region's nucleotide sequence